Please use this identifier to cite or link to this item:
標題: Bifidobacterium longum B7與金針菇水溶性多醣對C57BL/6JNarl小鼠調節血糖、血脂以及改善腸胃功能之影響
Effects of Bifidobacterium longum B7 and water-soluble polysaccharide from Flammulina velutipes on blood glucose, plasma lipids and gastrointestinal function in C57BL/6JNarl mice
作者: 吳泰駿
Wu, Tai-Jun
關鍵字: 乳酸菌
Lactic acid bacteria
Flammulina velutipes
glucose intolerance test
出版社: 食品暨應用生物科技學系所
引用: 丁予安、行政院、衛生署、國民健康局、中華民國血脂及動脈硬化學會(2003)。高血脂防治手冊: 國人血脂異常診療及預防指引。行政院衛生署國民健康局。 水野卓、川合正允(1997)。菇類的化學。國立編譯館編譯。 王文珍、周文能(1997)。有趣的真菌。國立自然科學博物館。 周寶嘉(2010)。文旦柚果皮多醣理化特性之研究。中興大學食品暨應用生物科技學系所。 林彥斌(1998)。香菇柄部、柳松菇基部與金針菇子實體的熱水和鹼液萃取物之品質評估及純化分析。中興大學食品暨應用生物科技學系所。 Abbott, D. A., Van Den Brink, J., Minneboo, I. M., Pronk, J. T., & Van Maris, A. J. (2009). Anaerobic homolactate fermentation with Saccharomyces cerevisiae results in depletion of ATP and impaired metabolic activity. FEMS yeast research, 9, 349-357. Abd El-Gawad, I. A., El-Sayed, E., Hafez, S., El-Zeini, H., & Saleh, F. (2005). The hypocholesterolaemic effect of milk yoghurt and soy-yoghurt containing bifidobacteria in rats fed on a cholesterol-enriched diet. International dairy journal, 15, 37-44. Adams, C. A. (2010). The probiotic paradox: live and dead cells are biological response modifiers. Nutrition research reviews, 23, 37-46. Aiba, Y., Suzuki, N., Kabir, A., Takagi, A., & Koga, Y. (1998). Lactic acid-mediated suppression of Helicobacter pylori by the oral administration of Lactobacillus salivarius as a probiotic in a gnotobiotic murine model. The American journal of gastroenterology, 93, 2097-2101. Aida, F., Shuhaimi, M., Yazid, M., & Maaruf, A. (2009). Mushroom as a potential source of prebiotics: a review. Trends in food science and technology, 20, 567-575. Aihara, K., Kajimoto, O., Hirata, H., Takahashi, R., & Nakamura, Y. (2005). Effect of powdered fermented milk with Lactobacillus helveticus on subjects with high-normal blood pressure or mild hypertension. Journal of the American college of nutrition, 24, 257-265. Alwan, A. (2011). Global status report on noncommunicable diseases 2010: World Health Organization, (Chapter Chapter). An, H. M., Park, S. Y., Lee, D. K., Kim, J. R., Cha, M. K., Lee, S. W., Lim, H. T., Kim, K. J., & Ha, N. J. (2011). Antiobesity and lipid-lowering effects of Bifidobacterium spp. in high fat diet-induced obese rats. Lipids health dis, 10, 116. Anderson, J. W., & Gilliland, S. E. (1999). Effect of fermented milk (yogurt) containing Lactobacillus acidophilus L1 on serum cholesterol in hypercholesterolemic humans. Journal of the American college of nutrition, 18, 43-50. Andersson, U., Branning, C., Ahrne, S., Molin, G., Alenfall, J., Onning, G., Nyman, M., & Holm, C. (2010). Probiotics lower plasma glucose in the high-fat fed C57BL/6J mouse. Beneficial microbes, 1, 189-196. Andrikopoulos, S., Blair, A. R., Deluca, N., Fam, B. C., & Proietto, J. (2008). Evaluating the glucose tolerance test in mice. American journal of physiology-endocrinology and metabolism, 295, 1323-1332. Angulo, P. (2007). GI epidemiology: nonalcoholic fatty liver disease. Alimentary pharmacology and therapeutics, 25, 883-889. Anstee, Q. M., & Goldin, R. D. (2006). Mouse models in non‐alcoholic fatty liver disease and steatohepatitis research. International journal of experimental pathology, 87, 1-16. Arimochi, H., Kinouchi, T., Kataoka, K., Kuwahara, T., & Ohnishi, Y. (1997). Effect of intestinal bacteria on formation of azoxymethane-induced aberrant crypt foci in the rat colon. Biochemical and biophysical research communications, 238, 753-757. Aroda, V. R., & Ratner, R. (2008). Approach to the patient with prediabetes. Journal of clinical endocrinology and metabolism, 93, 3259-3265. Axelsson, L. (2009). Lactic acid bacteria: Classification and physiology. Lactic acid bacteria: microbiological and functional aspects, 139, 1. Baird, J. D. (1973). Diabetes mellitus and obesity. Proceedings of the nutrition society, 32, 199-204. Barros, L., Cruz, T., Baptista, P., Estevinho, L. M., & Ferreira, I. C. (2008). Wild and commercial mushrooms as source of nutrients and nutraceuticals. Food and chemical toxicology, 46, 2742-2747. Begley, M., Hill, C., & Gahan, C. G. (2006). Bile salt hydrolase activity in probiotics. Applied and environmental microbiology, 72, 1729-1738. Berrada, N., Lemeland, J.-F., Laroche, G., Thouvenot, P., & Piaia, M. (1991). Bifidobacterium from fermented milks: survival during gastric transit. Journal of dairy science, 74, 409-413. Bouvy, M. (1997). Applied therapeutics: The clinical use of drugs. Pharmacy world and science, 19, 116-116. Brown, M. S., & Goldstein, J. L. (1983). Lipoprotein metabolism in the macrophage: implications for cholesterol deposition in atherosclerosis. Annual review of biochemistry, 52, 223-261. Cani, P. D., Bibiloni, R., Knauf, C., Waget, A., Neyrinck, A. M., Delzenne, N. M., & Burcelin, R. (2008). Changes in gut microbiota control metabolic endotoxemia-induced inflammation in high-fat diet–induced obesity and diabetes in mice. Diabetes, 57, 1470-1481. Cani, P. D., Possemiers, S., Van de Wiele, T., Guiot, Y., Everard, A., Rottier, O., Geurts, L., Naslain, D., Neyrinck, A., & Lambert, D. M. (2009). Changes in gut microbiota control inflammation in obese mice through a mechanism involving GLP-2-driven improvement of gut permeability. Gut, 58, 1091-1103. Carr, F. J., Chill, D., & Maida, N. (2002). The lactic acid bacteria: a literature survey. Critical reviews in microbiology, 28, 281-370. Chait, A., Brazg, R. L., Tribble, D. L., & Krauss, R. M. (1993). Susceptibility of small, dense, low-density lipoproteins to oxidative modification in subjects with the atherogenic lipoprotein phenotype, pattern B. The American journal of medicine, 94, 350-356. Challa, A., Rao, D. R., Chawan, C. B., & Shackelford, L. (1997). Bifidobacterium longum and lactulose suppress azoxymethane-induced colonic aberrant crypt foci in rats. Carcinogenesis, 18, 517-521. Chen, J., & Huang, X.-F. (2009). The effects of diets enriched in beta-glucans on blood lipoprotein concentrations. Journal of clinical lipidology, 3, 154-158. Clarke, J. M., Topping, D. L., & Suzuki, T. (2011). Bifidobacteria can protect from enteropathogenic infection through production of acetate. Nature, 469,543-547. Coppa, G., Zampini, L., Galeazzi, T., & Gabrielli, O. (2006). Prebiotics in human milk: a review. Digestive and liver disease, 38, 291-294. Cummings, J. H., Macfarlane, G. T., & Englyst, H. N. (2001). Prebiotic digestion and fermentation. The American journal of clinical nutrition, 73, 415-420. Danaei, G., Finucane, M. M., Lu, Y., Singh, G. M., Cowan, M. J., Paciorek, C. J., Lin, J. K., Farzadfar, F., Khang, Y.-H., & Stevens, G. A. (2011). National, regional, and global trends in fasting plasma glucose and diabetes prevalence since 1980: systematic analysis of health examination surveys and epidemiological studies with 370 country-years and 2.7 million participants. The Lancet, 378, 31-40. de Vrese, M., & Schrezenmeir, J. (2008). Probiotics, prebiotics, and synbiotics. Food biotechnology, Springer, 1-66. Despres, J.-P., & Lemieux, I. (2006). Abdominal obesity and metabolic syndrome. Nature, 444, 881-887. Drisko, J. A., Giles, C. K., & Bischoff, B. J. (2003). Probiotics in health maintenance and disease prevention. Alternative medicine review, 8, 143-155. Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. t., & Smith, F. (1956). Colorimetric method for determination of sugars and related substances. Analytical chemistry, 28, 350-356. Epstein, F. H., & Ross, R. (1999). Atherosclerosis—an inflammatory disease. New England journal of medicine, 340, 115-126. Fabbrini, E., Sullivan, S., & Klein, S. (2010). Obesity and nonalcoholic fatty liver disease: biochemical, metabolic, and clinical implications. Hepatology, 51,679-689. Folch, J., Lees, M., & Sloane-Stanley, G. (1957). A simple method for the isolation and purification of total lipids from animal tissues. The Journal of biological chemistry, 226, 497-509. Fukushima, M., Ohashi, T., Fujiwara, Y., Sonoyama, K., & Nakano, M. (2001).Cholesterol-lowering effects of maitake (Grifola frondosa) fiber, shiitake (Lentinus edodes) fiber, and enokitake (Flammulina velutipes) fiber in rats. Experimental biology and medicine, 226, 758-765. Fuller, R. (1973). Ecological studies on the lactobacillus flora associated with the crop epithelium of the fowl. Journal of applied microbiology, 36, 131-139. Fulop, T., Tessier, D., & Carpentier, A. (2006). The metabolic syndrome. Pathologie biologie, 54, 375-386. Gibson, G. R., Beatty, E. R., Wang, X., & Cummings, J. H. (1995). Selective stimulation of bifidobacteria in the human colon by oligofructose and inulin. Gastroenterology, 108, 975-982. Gibson, G. R., & Fuller, R. (2000). Aspects of in vitro and in vivo research approaches directed toward identifying probiotics and prebiotics for human use. The Journal of nutrition, 130, 391S-395S. Gibson, G. R., Probert, H. M., Van Loo, J., Rastall, R. A., & Roberfroid, M. B. (2004). Dietary modulation of the human colonic microbiota: updating the concept of prebiotics. Nutrition research reviews, 17, 259-275. Greany, K. A., Nettleton, J. A., Wangen, K. E., Thomas, W., & Kurzer, M. S. (2004). Probiotic consumption does not enhance the cholesterol-lowering effect of soy in postmenopausal women. The Journal of nutrition, 134, 3277-3283. Guarino, A., Vecchio, A. L., & Canani, R. B. (2009). Probiotics as prevention and treatment for diarrhea. Current opinion in gastroenterology, 25, 18-23. Guo, F., Williams, B., Kwakkel, R., Li, H., Li, X., Luo, J., Li, W., & Verstegen, M. (2004). Effects of mushroom and herb polysaccharides, as alternatives for an antibiotic, on the cecal microbial ecosystem in broiler chickens. Poultry science, 83,175-182. Hansson, G. K. (2001). Immune mechanisms in atherosclerosis. Arteriosclerosis, thrombosis, and vascular biology, 21, 1876-1890. Harmon, S. M., Kautter, D. A., & Peeler, J. T. (1971). Improved medium for enumeration of Clostridium perfringens. Applied microbiology, 22, 688-692. Holum, J. R., & Olmsted, S. L. (1994). Fundamentals of general, organic, and biological chemistry: John Wiley and Sons New York. Holzapfel, W. H., Haberer, P., Snel, J., & Schillinger, U. (1998). Overview of gut flora and probiotics. International journal of food microbiology, 41, 85-101. Hosoda, M., Hashimoto, H., He, F., Morita, H., & Hosono, A. (1996). Effect of administration of milk fermented with Lactobacillus acidophilus LA-2on fecal mutagenicity and microflora in the human intestine. Journal of dairy science, 79, 745-749. Hosono, A. (1999). Bile tolerance, taurocholate deconjugation, and binding of cholesterol by Lactobacillus gasseri strains. Journal of dairy science, 82, 243-248. Ishida, B., Blanche, P., Nichols, A., Yashar, M., & Paigen, B. (1991). Effects of atherogenic diet consumption on lipoproteins in mouse strains C57BL/6 and C3H. Journal of lipid research, 32, 559-568. Jernas, M., Palming, J., Sjoholm, K., Jennische, E., Svensson, P.-A., Gabrielsson, B. G., Levin, M., Sjogren, A., Rudemo, M., & Lystig, T. C. (2006). Separation of human adipocytes by size: hypertrophic fat cells display distinct gene expression. The FASEB journal, 20, 1540-1542. Jeun, J., Kim, S., Cho, S.-Y., Jun, H.-j., Park, H.-J., Seo, J.-G., Chung, M.-J., & Lee, S.-J. (2010). Hypocholesterolemic effects of Lactobacillus plantarum KCTC3928 by increased bile acid excretion in C57BL/6 mice. Nutrition, 26, 321-330. Jones, M. L., Chen, H., Ouyang, W., Metz, T., & Prakash, S. (2004). Microencapsulated genetically engineered Lactobacillus plantarum 80 (pCBH1) for bile acid deconjugation and its implication in lowering cholesterol. BioMed research international, 2004, 61-69. Kahn, B. B., & Flier, J. S. (2000). Obesity and insulin resistance. Journal of clinical investigation, 106, 473-481. Kalliomaki, M., Salminen, S., Arvilommi, H., Kero, P., Koskinen, P., & Isolauri, E. (2001). Probiotics in primary prevention of atopic disease: a randomized placebo-controlled trial. The Lancet, 357, 1076-1079. Kanmani, P., Satish Kumar, R., Yuvaraj, N., Paari, K., Pattukumar, V., & Arul, V. (2013). Probiotics and Its Functionally Valuable Products—A Review. Critical reviews in food science and nutrition, 53, 641-658. Kimoto, H., Ohmomo, S., & Okamoto, T. (2002). Cholesterol removal from media by lactococci. Journal of dairy science, 85, 3182-3188. Kirk, E., Moe, G., Caldwell, M., Lernmark, J., Wilson, D., & LeBoeuf, R. (1995). Hyper-and hypo-responsiveness to dietary fat and cholesterol among inbred mice: searching for level and variability genes. Journal of lipid research, 36, 1522-1532. Koebnick, C., Wagner, I., Leitzmann, P., Stern, U., & Zunft, H. F. (2003). Probiotic beverage containing Lactobacillus casei Shirota improves gastrointestinal symptoms in patients with chronic constipation. Canadian journal of gastroenterology, 17, 655-660. Konig, H., Unden, G., & Frohlich, J. (2009). Biology of microorganisms on grapes, in must and in wine: Springer. Konno, K. (1995). Biologically active components of poisonous mushrooms. Food reviews international, 11, 83-107. Kotsis, V., Stabouli, S., Bouldin, M., Low, A., Toumanidis, S., & Zakopoulos, N. (2005). Impact of obesity on 24-hour ambulatory blood pressure and hypertension. Hypertension, 45, 602-607. Lambert, J. M., Bongers, R. S., de Vos, W. M., & Kleerebezem, M. (2008). Functional analysis of four bile salt hydrolase and penicillin acylase family members in Lactobacillus plantarum WCFS1. Applied and environmental microbiology, 74, 4719-4726. Lazar, M. A. (2005). How obesity causes diabetes: not a tall tale. Science, 307, 373-375. Leung, M., Fung, K., & Choy, Y. (1997). The isolation and characterization of an immunomodulatory and anti-tumor polysaccharide preparation from Flammulina velutipes. Immunopharmacology, 35, 255-263. Lin, P., & Chang, C. (1994). Endothelium dysfunction in cardiovascular diseases. Chang Gung medical journal, 17, 198. Lionetti, E., Miniello, V., Castellaneta, S., Magista, A., De Canio, A., Maurogiovanni, G., Ierardi, E., Cavallo, L., & Francavilla, R. (2006). Lactobacillus reuteri therapy to reduce side‐effects during anti‐Helicobacter pylori treatment in children: a randomized placebo controlled trial. Alimentary pharmacology and therapeutics, 24, 1461-1468. Liong, M., & Shah, N. (2005). Acid and bile tolerance and cholesterol removal ability of lactobacilli strains. Journal of dairy science, 88, 55-66. Liong, M., & Shah, N. (2006). Effects of a Lactobacillus casei synbiotic on serum lipoprotein, intestinal microflora, and organic acids in rats. Journal of dairy science, 89, 1390-1399. Lubbadeh, W., Haddadin, M., Al-Tamimi, M., & Robinson, R. (1999). Effect on the cholesterol content of fresh lamb of supplementing the feed of Awassi ewes and lambs with Lactobacillus acidophilus. Meat science, 52, 381-385. Marco, M. L., Pavan, S., & Kleerebezem, M. (2006). Towards understanding molecular modes of probiotic action. Current opinion in biotechnology, 17,204-210. Marteau, P., Cuillerier, E., Meance, S., Gerhardt, M., Myara, A., Bouvier, M., Bouley, C., Tondu, F., Bommelaer, G., & Grimaud, J. (2002). Bifidobacterium animalis strain DN‐173 010 shortens the colonic transit time in healthy women: a double‐blind, randomized, controlled study. Alimentary pharmacology and therapeutics, 16, 587-593. Matsuzaki, T., Yamazaki, R., Hashimoto, S., & Yokokura, T. (1997). Antidiabetic effects of an oral administration of Lactobacillus casei in anon-insulin-dependent diabetes mellitus (NIDDM) model using KK-Ay mice. Endocrine journal, 44, 357. Mattila, P., Suonpaa, K., & Piironen, V. (2000). Functional properties of edible mushrooms. Nutrition, 16, 694-696. Mellitus, D. (2006). Diagnosis and classification of diabetes mellitus. Diabetes care, 29: S43. Metzger, B. E., & Coustan, D. R. (1998). Summary and recommendations of the fourth international workshop-conference on gestational diabetes mellitus. Diabetes care, 21, B161. Miller, G. L. (1959). Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical chemistry, 31, 426-428. Morishita, Y., Oowada, T., Ozaki, A., & Mizutani, T. (2002). Galactooligosaccharide in combination with Bifidobacterium and Bacteroides affects the population of Clostridium perfringens in the intestine of gnotobiotic mice. Nutrition research, 22, 1333-1341. Mottet, C., & Michetti, P. (2005). Probiotics: wanted dead or alive. Digestive and liver disease, 37, 3-6. Mussatto, S. I., & Mancilha, I. M. (2007). Non-digestible oligosaccharides: a review.Carbohydrate polymers, 68, 587-597. Musso, G., Gambino, R., Bo, S., Uberti, B., Biroli, G., Pagano, G., & Cassader, M. (2008). Should nonalcoholic fatty liver disease be included in the definition of metabolic syndrome? A cross-sectional comparison with Adult Treatment Panel III criteria in nonobese nondiabetic subjects. Diabetes care, 31, 562-568. Naito, E., Yoshida, Y., Makino, K., Kounoshi, Y., Kunihiro, S., Takahashi, R., Matsuzaki, T., Miyazaki, K., & Ishikawa, F. (2011). Beneficial effect of oral administration of Lactobacillus casei strain Shirota on insulin resistance in diet‐induced obesity mice. Journal of applied microbiology, 110, 650-657. Nishina, P. M., Verstuyft, J., & Paigen, B. (1990). Synthetic low and high fat diets for the study of atherosclerosis in the mouse. Journal of lipid research, 31,859-869. Nobaek, S., Johansson, M.-L., Molin, G., Ahrne, S., & Jeppsson, B. (2000). Alteration of intestinal microflora is associated with reduction in abdominal bloating and pain in patients with irritable bowel syndrome. The American journal of gastroenterology, 95, 1231-1238. Oatley, J. T., Rarick, M. D., Ji, G. E., & Linz, J. E. (2000). Binding of aflatoxin B1 to bifidobacteria in vitro. Journal of food protection, 63, 1133-1136. Ooi, L.-G., & Liong, M.-T. (2010). Cholesterol-lowering effects of probiotics and prebiotics: a review of in vivo and in vitro findings. International journal of molecular sciences, 11, 2499-2522. Parvez, S., Malik, K., Ah Kang, S., & Kim, H. Y. (2006). Probiotics and their fermented food products are beneficial for health. Journal of applied microbiology, 100, 1171-1185. Pharmaceutiques, U. d. L. (1995). Dietary modulation of the human colonie microbiota: introducing the concept of prebiotics. Journal of nutrition, 125, 1401-1412. Reid, G., Jass, J., Sebulsky, M. T., & McCormick, J. K. (2003). Potential uses of probiotics in clinical practice. Clinical microbiology reviews, 16, 658-672. Salminen, S., & Salminen, E. (1997). Lactulose, lactic acid bacteria, intestinal microecology and mucosal protection. Scandinavian journal of gastroenterology, 222, 45. Savage, D. (1982). Mechanisms by which indigenous microorganisms colonize gastrointestinal epithelial surfaces. Progress in food and nutrition science, 7, 65-74. Sibel Akalin, A., Gonc, S., & Duzel, S. (1997). Influence of yogurt and acidophilus yogurt on serum cholesterol levels in mice. Journal of dairy science, 80, 2721-2725. Sookoian, S., & Pirola, C. J. (2008). Non-alcoholic fatty liver disease is strongly associated with carotid atherosclerosis: a systematic review. Journal of hepatology, 49, 600-607. Synytsya, A., Mičkova, K., Synytsya, A., Jablonsky, I., Spěvaček, J., Erban, V., Kovařikova, E., & Čopikova, J. (2009). Glucans from fruit bodies of cultivated mushrooms Pleurotus ostreatus and Pleurotus eryngii: structure and potential prebiotic activity. Carbohydrate polymers, 76,548-556. Tabuchi, M., Ozaki, M., Tamura, A., Yamada, N., Ishida, T., Hosoda, M., & Hosono, A. (2003). Antidiabetic effect of Lactobacillus GG in streptozotocin-induced diabetic rats. Bioscience, biotechnology, and biochemistry, 67, 1421-1424. Tanti, J., Gual, P., Gremeaux, T., Gonzalez, T., Barres, R., & Le Marchand-Brustel, Y. (2004). Alteration in insulin action: role of IRS-1 serine phosphorylation in the retroregulation of insulin signalling. Annales d''endocrinologie , 65, 43-8 Targher, G., Bertolini, L., Poli, F., Rodella, S., Scala, L., Tessari, R., Zenari, L., & Falezza, G. (2005). Nonalcoholic fatty liver disease and risk of future cardiovascular events among type 2 diabetic patients. Diabetes, 54,3541-3546. Targher, G., Bertolini, L., Rodella, S., Tessari, R., Zenari, L., Lippi, G., & Arcaro, G. (2007). Nonalcoholic fatty liver disease is independently associated with an increased incidence of cardiovascular events in type 2 diabetic patients. Diabetes care, 30, 2119-2121. Vanderhoof, J. A., & Young, R. J. (1998). Use of probiotics in childhood gastrointestinal disorders. Journal of pediatric gastroenterology and nutrition, 27, 323-332. Vogel, R. A. (1997). Coronary risk factors, endothelial function, and atherosclerosis: a review. Clinical cardiology, 20, 426-432. Vonk, R. J., Reckman, G. A., Harmsen, H. J., & Priebe, M. G. (2012). Probiotics and Lactose Intolerance. Chapter 7, 149-160. Wellen, K. E., & Hotamisligil, G. S. (2005). Inflammation, stress, and diabetes. Journal of clinical investigation, 115, 1111-1119. Wu, D.-m., Duan, W.-q., Liu, Y., & Cen, Y. (2010). Anti-inflammatory effect of the polysaccharides of golden needle mushroom in burned rats. International journal of biological macromolecules, 46, 100-103. Xiao, J., Kondo, S., Takahashi, N., Miyaji, K., Oshida, K., Hiramatsu, A., Iwatsuki, K., Kokubo, S., & Hosono, A. (2003). Effects of milk products fermented by Bifidobacterium longum on blood lipids in rats and healthy adult male volunteers. Journal of dairy science, 86, 2452-2461. Zimmet, P., Alberti, K., & Shaw, J. (2001). Global and societal implications of the diabetes epidemic. Nature, 414, 782-787
摘要: 乳酸菌被認為具有多種的保健功效,其中包括抗發炎、降血脂、調節免疫、減緩過敏、減緩乳糖不耐症。另外有研究提到菇類多醣具有降血糖、降血脂、改善腸道菌相的功效。本研究為探討Bifidobacterium longum B7結合不同劑量的金針菇水溶性多醣。對於C57BL/6JNarl小鼠血脂、血糖、腸胃道功能的影響。實驗組餵食高脂飲食持續8週後,結果顯示高劑量多醣和B. longum B7能明顯減少體重增加、減少腹部脂肪累積、降低血液中總膽固醇、LDL-C (p <0.05)。B. longum B7結合多醣能提高糞便膽固醇排出量(p <0.05),並且B. longum B7和多醣能降低肝臟膽固醇、肝臟三酸甘油脂以及禁食血糖(p <0.05),但無法改善葡萄糖耐受性不佳的情形。糞便菌相顯示,B. longum B7和多醣能促進腸道乳酸菌和Bifidobacterium spp.生長(p <0.05)。因此本實驗證實乳酸以及金針菇水溶性多醣能調節血脂、改善腸胃功能。
Lactic acid bacteria (LAB) have been considered as probiotics to improve and maintain health, which can reduce inflammatory response, reducing serum cholesterol, regulating immunity, moderating allergy symptoms and reducing lactose intolerance. Other studies indicated mushroom polysaccharides contain many health benifits, including the decrease in serum cholesterol and blood glucose level and modification of intestinal microbiota.The study aims to evaluate the effect of Bifidobacterium longum B7 and water-soluble polysaccharide from Flammulina velutipes on plasma lipids, glucose and gastrointestinal function in C57BL/6JNarl mice. The experimental groups were fed the high fat diet (HFD) for 8 weeks, Results indicate that high dose of polysaccharide and B. longum B7 reduced body weight gain, accumulation of abdominal fat, serum total cholesterol and LDL-C(p <0.05). B. longum B7 combined with polysaccharides increased fecal cholesterol excretion(p <0.05). B. longum B7 and polysaccharides reduced hepatic cholesterol, triglycerides and fasting blood glucose(p <0.05). However, there were no significant differences in oral glucose intolerance test. Besides, administration of B. longum B7 and polysaccharides also promoted the growth of LAB and Bifidobacterium spp(p <0.05). In conclusion, these data indicate that B. longum B7 and water-soluble polysaccharide (Flammulina velutipes) have hypolipidemic effect and improvement of gastrointestinal function.
其他識別: U0005-2308201315440200
Appears in Collections:食品暨應用生物科技學系



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.