Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/52186
標題: 利用難消化性麥芽糊精建立一種減低大鼠體脂肪之實驗模式
Development of body fat reduction evaluation models in rats using resistant maltodextrin
作者: 郭婉宜
Kuo, Wan-I
關鍵字: 肥胖
obesity
高脂飲食
膳食纖維
不易形成體脂肪
high fat diet
dietary fiber
body fat reduction
出版社: 食品暨應用生物科技學系所
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摘要: 為了探討動物實驗的高脂飲食模式中,實驗是否會因為飼料特性或是實驗樣品的適口性對動物攝食量的影響,而導致不易形成體脂肪評估結果的誤差,造成高估或低估實驗因子的作用,本研究使用分別為含油脂重量百分比23%的A-HF組、添加煉乳含油脂重量百分比23%的B-HF組、含油脂重量百分比40%的C-HF組和添加煉乳含油脂重量百分比40%的D-HF組,以及不同劑量之纖維樣品(fibersol),針對大鼠攝食量、體重增加量及體脂肪形成等進行探討,並對適當的高脂飲食模式加以討論。動物體重結果顯示,在大部分的高脂模式與控制組比較皆可以明顯(P<0.05)提高大鼠的每日體重增加量:A-HF組提高23.2%、B-HF組提高34.1%和D-HF組提高26.7%。而動物攝食量方面研究結果顯示,除了添加煉乳的B-HF組僅下降2.4%外,其餘高脂飲食組別的攝食量皆顯著(P<0.05)降低,包括A-HF組下降15.7%、C-HF組下降31%,D-HF組則是下降11.2%,攝食量的不同可能導致影響減低體脂肪效果評估之客觀性。 有關熱量攝取方面,攝食高脂飲食的A-HF組、B-HF組、C-HF組和D-HF組每日平均攝取熱量皆顯著(P<0.05)較控制組高,分別高14%、25.5%、21.2%和44.3%。而各組間有無添加fibersol對攝取熱量皆無顯著差異。煉乳的添加在高脂模式可以顯著(P<0.05)增加大鼠的每日攝食量及脂肪攝食量。亦即,同樣的油脂攝取百分比,飼料配方的組成不同會造成實驗結果的不同。體脂肪方面,結果顯示在相同油脂含量的模式下,額外餵食添加煉乳的飼料均有較高的體脂肪累積量。有關纖維樣品對添加煉乳的高脂飲食組(23%油脂)之影響,可以發現隨著纖維的劑量增加,體脂肪之生成量有顯著(P<0.05)下降的趨勢:一倍劑量下降9%、兩倍劑量則下降25.2%;而在同樣油脂含量(23%)下,纖維的添加可以造成體脂肪顯著(P<0.05)下降:一倍劑量下降14.2%、兩倍劑量下降23.1%,但兩者並沒有顯著差異。另一方面,40%油脂的高脂飲食,纖維樣品對於體脂肪之生成量,在兩倍劑量下顯著(P<0.05)降低18.5%;而在40%油脂有添加煉乳的高脂飲食之組別中,纖維樣品的添加對於動物體脂肪並沒有明顯降低的影響。整體而言,從攝食量及纖維樣品對體脂肪的影響的結果判斷,23%油脂有添加煉乳的高脂飲食組為較佳的實驗動物肥胖模式之飲食配方。
The aim of this study was to develop an experimental model for the evaluation of body-fat reduction. By feeding rats with dietary fiber and high-fat diets of different formula, their food intakes, weight gains and body fat percentages were investigated. The different high-fat diet formulas included 1) A: 23% fat diet, 2) B: 23% fat diet with condensed milk, 3) C: 40% fat diet, and 4) D: 40% fat diet with condensed milk. The results showed that the food intakes of both the A-HF and C-HF groups were significantly (P<0.05) decreased. More specifically, the food intakes of A-HF, C-HF, and D-HF groups were decreased by 13.5%, 25.3%, and 9.7%, respectively. However, the food intake of B-HF group was not found to be slightly different from the control group. In addition, it was found that the feeding of diets with condensed milk could significantly (P<0.05) increase the food intake of rats. In respect of the body fat, the results showed that the addition of condensed milk in diets of the same fat levels significantly (P<0.05) lead to an increase in the body fat. When a high dosage of dietary fiber was added, the body fat of B-2X group was significantly (P <0.05) decreased by 25.2% as compared with B-HF group. In contrast, no apparent decreases in body fat were observed in all the other groups upon the addition of dietary fiber. In this study, the feeding of high-fat diet supplemented with dietary fiber could result in a reduction of body fat in rats, and the 23% fat high-fat diet with condensed milk (formula B) was found to be a suitable model for the evaluation of body fat reduction.
URI: http://hdl.handle.net/11455/52186
其他識別: U0005-2609201220270000
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-2609201220270000
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