Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/52419
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dc.contributor.author茅繼良zh_TW
dc.contributor.author陳昭鈴zh_TW
dc.contributor.other行政院農業委員會zh_TW
dc.contributor.other中興大學獸醫學系zh_TW
dc.date2001zh_TW
dc.date.accessioned2014-06-06T08:56:47Z-
dc.date.available2014-06-06T08:56:47Z-
dc.identifier89科技-6.1-檢-68zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/52419-
dc.description.abstract傳統之藥物殘留所使用的檢測方法如Bioassay, HPLC, GC等方法,需要很多步驟,花費很長時間才能檢測出結果,β-lactam藥物包括ampicillin, penicillin, amoxicillin等多種抗生素,為檢測β-lactam抗生素之殘留,以擴大β-lactam類藥物殘留初部篩選工作,有必要開發β-lactam抗生素殘留快速檢測試劑.實驗步驟包含: 1. 對抗β-lactam藥物單株抗體之製造 2. β-lactam藥物及HRP呈色劑之衍生物製備 3. 合成衍生物與蛋白結合體之製 4. 進行實驗室檢驗試劑品質測 5. 進行田間樣品檢驗試劑品質測試 6. 完成檢驗試劑開發,建立檢驗方法.開發完成之β-lactam抗生素殘留快速檢驗試劑可作為擴大篩選檢驗上市前禽畜產品之檢測工作,並可做為畜牧場獸醫師進行快速篩選藥物殘留之禽畜產品上市之用,有效防範藥物殘留.zh_TW
dc.description.abstractThere are many methods for detection of Beta-lactam including TLC, HPLC, GC, LC, and CE. However, the sample to be tested need to extracted first and it takes a lot of time. In order to screen large amount of samples it is necessary to find a rapid, sensitive test, ELISA might be a good idea choice for this purpose. To develop ELISA kit, the following steps should be performed: 1. Production of monoclone antibody. 2. Conjugation of beta-lactam with HRP. 3. Conjugation of beta-lactam with macromolecule. 4. Test quality of ELISA kit. 5. Field trial of kit. 6. Establishment of standard test procedure Hopefully, the kit can be used in large scale to screen the product of meat and milk before marketing, to reduce the risk of drug contamination in these product.en_US
dc.language.isozh_TWzh_TW
dc.relation.urihttp://grbsearch.stpi.narl.org.tw/GRB/result.jsp?id=563151&plan_no=89%E7%A7%91%E6%8A%80-6.1-%E6%AA%A2-68&plan_year=89&projkey=PG8909-0608&target=plan&highStr=*&check=0&pnchDesc=%E7%95%9C%E7%94%A2%E5%93%81%CE%B2-lactam%E6%8A%97%E7%94%9F%E7%B4%A0%E6%AE%98%E7%95%99%E5%BF%AB%E9%80%9F%E6%AA%A2%E9%A9%97%E8%A9%A6%E5%8A%91%E4%B9%8B%E9%96%8B%E7%99%BCen_US
dc.subject應用研究zh_TW
dc.subjectBeta-lactamen_US
dc.subject食品科技, 畜牧獸醫類zh_TW
dc.subject酵素連結免疫吸附檢測法zh_TW
dc.subject藥物殘留zh_TW
dc.subjectELISAen_US
dc.subjectDrug Residueen_US
dc.title畜產品β-lactam抗生素殘留快速檢驗試劑之開發zh_TW
dc.titleDevelopment of ELISA Kit Detecting Βeta-Lactam Residue in Animal Productsen_US
dc.typeResearch Reportszh_TW
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