Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/52693
標題: 以Multiplex RT-PCR與PCR/RFLP技術快速檢測及區分H5、H6亞型禽流感病毒及高病原性新城病毒
Differentiation of H5, H6 Avian Influenza Virus and Very Virulent Newcastle Disease Virus by Multiplex Rt-Pcr and Pcr-Rflp
作者: 張伯俊
沈瑞鴻
謝快樂
關鍵字: 畜牧獸醫類
應用研究
摘要: 本計畫之目標,為建立之multiplex RT-PCR與RT-PCR/RFLP技術,以快速鑑定及區分H5N1、H5N2、H6N1禽流感病毒,以及高病原性新城病毒。當發生雞隻大量死亡現象時,可利用本技術於4-5小時內,快速診斷並確定有無H5N1、H6N1,或vv-NDV或其它流感病毒存在,本技術之建立,對於國內禽流感及高病原性新城病之防疫工作,將有重大助益。
The goal of this study is to establish multiplex RT-PCR and RT-PCR/RFLP to detection and differentiation between H5N1, N5N2 and H6N1 viruses and very virulent Newcastle disease viruses (vv-NDV). When diseases causing a high mortality of chickens occur, this methodology could be used to detect and differentiate between H5N1, N5N2, H6N1 viruses and vv-NDV in 4-5 hours. The establishment of this methodology will be helpful to control and prevent avian influenza and Newcastle disease in this country.
URI: http://hdl.handle.net/11455/52693
其他識別: 97農科-14.6.1-檢-B4(2)
文章連結: http://grbsearch.stpi.narl.org.tw/GRB/result.jsp?id=1609365&plan_no=97%E8%BE%B2%E7%A7%91-14.6.1-%E6%AA%A2-B4%282%29&plan_year=97&projkey=PW9706-0788&target=plan&highStr=*&check=0&pnchDesc=%E4%BB%A5Multiplex+RT-PCR%E8%88%87PCR%2FRFLP%E6%8A%80%E8%A1%93%E5%BF%AB%E9%80%9F%E6%AA%A2%E6%B8%AC%E5%8F%8A%E5%8D%80%E5%88%86H5%E3%80%81H6%E4%BA%9E%E5%9E%8B%E7%A6%BD%E6%B5%81%E6%84%9F%E7%97%85%E6%AF%92%E5%8F%8A%E9%AB%98%E7%97%85%E5%8E%9F%E6%80%A7%E6%96%B0%E5%9F%8E%E7%97%85%E6%AF%92
Appears in Collections:獸醫學系所

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