請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/54380
標題: 重要檢疫害蟲種類鑑定之DNA晶片研發
Research and Development of a DNA Chip for Species Identification of Important Quarantine Pest Mites
作者: 路光暉
張淑貞
關鍵字: 生物技術, 植物保護類
葉?
tetranychid mites
根?
桃蛀果蛾
粒線體COI片段
生物晶片
技術發展
bulb mite
Carposina sasakii
mitochondrial cytochrome oxidase subunit I (COI) gene
biochip
摘要: The tetranychid mites and bulb mites are important pests of international quarantine. They attack a wide variety of crops and cause economic damage. They are minute in size and not easily distinguishable by traditional classification methods. This study attempted to use DNAs for the identification of these pests. Polymerase chain reaction (PCR) will be used to amplify mitochondrial cytochrome oxidase subunit I (COI) gene of these pests. Species-specific oligonuleotide probe based on these sequences gave the biochip method could be used to identify the mites on imported apples and bulbs.
葉?及根?中的許多種類為國際間重要檢疫害蟲,危害許多農作物造成嚴重經濟損失;體型微小難以分辨。本計畫主要目標在於建立重要檢防疫葉?及根?之快速鑑定技術。增幅並定序葉?及根?的粒線體COI片段,以其DNA序列差異處,設計具種專一性之探針,建立害?生物晶片檢測技術,將可應用於蘋果及花卉種球的葉?、根?種類檢疫所需。
URI: http://hdl.handle.net/11455/54380
其他識別: 99農科-9.3.1-檢-B1(7)
文章連結: http://grbsearch.stpi.narl.org.tw/GRB/result.jsp?id=2068005&plan_no=99%E8%BE%B2%E7%A7%91-9.3.1-%E6%AA%A2-B1%287%29&plan_year=99&projkey=PW9905-0289&target=plan&highStr=*&check=0&pnchDesc=%E9%87%8D%E8%A6%81%E6%AA%A2%E7%96%AB%E5%AE%B3%E8%9F%B2%E7%A8%AE%E9%A1%9E%E9%91%91%E5%AE%9A%E4%B9%8BDNA%E6%99%B6%E7%89%87%E7%A0%94%E7%99%BC
顯示於類別:昆蟲學系

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