請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/5499
標題: 高溫好氧油脂分解菌動力參數研究
A Kinetic Parameter Study of a Thermophilic Aerobic System Treating High Strength Oily Wasterwaters
作者: 沈育資
Shen, Yu-Tzu
關鍵字: Thermophilics
reaction kinetic
Thermus sp.
Microbacterium sp.
Thermus sp.
Microbacterium sp.
出版社: 環境工程學系所
引用: 吳永興 (2004) 自發性高溫好氧處理程序之研究:系統參數測定演算法之開發,博士論文,國立中興大學環境工程學研究所,臺中 李季眉 (1992) 環境微生物實驗,中興大學,臺中 胡苔莉 (1987) 微生物學實驗,國立編譯館,臺北 韓麗明,吳常豪 (1977) 工業廢水之理論、實務及處理,徐氏基金會,臺北 顏國欽 (1993) 食品油脂學(上),中興大學,臺中 American Public Health Association (APHA), American Water Works Association (AWWA), and Water Environment Federaation (WEF) (1998) Standars for Examination of Water and Wastewater – Part 5210:Biochemical Oxygen Demand (BOD) Arpigny, J. L. and K. E. Jaeger (1999) “Bacterial lipolytic enzymes: classification and properties” Biochem J., 343:177-83 Ateslier, Z. B. B. and K. Metin (2006) “Production and partial characterization of a novel thermostable esterase from a thermophilic Bacillus sp.” Enzyme and Microbial. 38:628-635 Baker, H., O. Frank, I. Pasher, B. Black, SH. Hutner, and H. Sobotka(1960)“Growth requirements of 94 strains of thermophilic bacilli” Can J Microbiol 6:557-63 Becker, P., D. Köster, M. N. Popov, S. Markossian, G. Antranikian, and H. 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摘要: 摘 要 本研究以桃園縣觀音工業區某食品公司的高溫油脂污泥為菌種來源,經實驗室以小規模反應槽長期馴養後,利用純種培養技術分離純化菌株,並委託某生物科技公司將其基因序列定序,將定序結果上NCBI網站比對可能菌株,並對照傳統微生物鑑定實驗結果以確認菌株。確認菌株後,以橄欖油做為基質,利用不同基質濃度的批次實驗,探討高溫油脂分解菌的反應動力,並以實驗所得結果比對文獻中的微生物生長動力模式,探討可用於評估高溫好氧油脂分解菌的反應動力模式。 純種培養所分離純化的兩株菌,將菌種基因序列定序結果上NCBI網站比對,並與傳統微生物實驗對照後,分別為Thermus sp.與Microbacterium sp.。 以Thermus sp.進行批次實驗,基質濃度介於30 ~ 30000 mg/L TOC時,隨著基質濃度的提高Thermus sp.的µinitial亦有增加的趨勢,但當基質濃度大於3000 mg/L TOC時,µinitial呈現下降的趨勢,推測是因基質濃度高於一限制值後,高基質濃度反而會抑制微生物生長。批次實驗基質濃度為30、300、3000、30000 mg/L TOC,而µinitial分別為0.97、3.89、3.66、1.59 mg-cell/mg-cell-day。 將批次實驗結果套用文獻中的模式,利用Andrews的抑制方程式以數值分析所得結果µmax為4.95 mg-cell/mg-cell-day ,Ks為97 mg/L TOC,Ki為12000 mg/L TOC;利用變異數分析,f值為0.56,大於顯著值0.53,顯示Andrews方程式可適用於評估本研究系統中的反應動力參數。
The research utilized the thermophilic wasted sludge colleeded from a food company in Guanyin Industrial Park as the micbacterial resource. After the acclionation with a lab-scale reactor for more than six months, obtaining the pure culture strain of bacteria was identified through the process of gene mapping by a bio-technological company. Then, the result of was used in comparison with some possible strains of bacteria on NCBI website. The results of traditional microbial identification experiments were also used to confirm the strain of bacteria. After the identification process, olive oil was used as the substrate for a series of batch experiments at different substrate concentrations. This research employed some microbial growth kinetic models to estimates the growth and decay of the bacteria obtained from the pure culture study. Two strains of bacteria was identified from the pure culture study were identified with the results on NCBI website and the results from traditional microbial identification experiments. Two species were identified, Thermus sp. and Microbacterium sp.. Pure Thermus sp. was used as the pure culture in batch experiment at the substrate concentrations in the range of 30 to 30000 mg/L TOC. The results indicated an increase in the substrate concentrations increased the µinitial when Thermus sp. was used as the microorgomisun. When the substrate concentration was higher than 3000 mg/L TOC, µinitial significant decreased because of the effect of substrate inhibition. When the concentrations of the substrate were 30, 300, 3000, and 30000 mg/L TOC, µinitial was 0.97, 3.89, 3.66, and 1.59 mg-cell/mg-cell-day, respectively. This study used Andrews' inhibition equation to simulate the result of the batch experiments. The values of µmax 4.95 mg-cell/mg-cell-day, Ks 97 mg/L TOC, and Ki were 12000 mg/L TOC, respectively. The result showed Andrew's equation was adaptable to estimate the kinetic parameters in this system.
URI: http://hdl.handle.net/11455/5499
其他識別: U0005-2508200813345600
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-2508200813345600


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