請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/5505
標題: 台灣中部地區空氣懸浮微粒之水溶性及脂溶性萃取物誘發人類乳癌細胞之細胞毒性和DNA氧化損害
Induction of cytotoxic response and oxidative DNA damage in human breast cancer cell lines by water- and solvent-extractable particulate matter in central Taiwan
作者: 劉懿賢
Liu, Yi-Shian
關鍵字: Particulate matter
Oxidative stress
Gene expression
出版社: 環境工程學系所
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摘要: 空氣懸浮微粒是具有健康危害效應的物質。文獻指出,空氣懸浮微粒上所吸附的脂溶性、水溶性及其他物質,是造成細胞胞內氧化壓力累積生成的重要因素之一。此外文獻亦提到,氧化壓力會造成人體老化、內分泌失調以及癌症等疾病。因此探討空氣懸浮微粒形成氧化壓力以及細胞毒性的機制,可以協助了解空氣懸浮微粒致細胞毒性及致癌作用機轉。本研究目的主要是去探討,曝露在空氣懸浮微粒之水溶性萃取樣本下,細胞內部是否為經由代謝活化效應誘發基因表現失衡,進而造成細胞胞內氧化壓力、DNA損害以及細胞死亡。本研究利用多種生物性指標,包括細胞存活率測試(SRB assay)、氧化壓力測定法(Fluorescence assay)、DNA單股斷裂分析法(COMET assay)以及西方墨點法(Western blot),針對懸浮微粒可能產生之細胞毒性、氧化壓力、基因損害以及基因表現進行研究與分析。根據實驗結果顯示,曝露在空氣懸浮微粒水溶性萃取物質下,不論是MDA-MB-231細胞還是MCF-7細胞均無明顯的細胞毒性效應生成。研究結果也顯示出,曝露於空氣懸浮微粒水溶性萃取物2小時下即有氧化壓力顯著上升。在懸浮微粒誘發DNA單股損害部分,三個採樣點的水溶性萃取液均能造成兩種乳癌細胞顯著之DNA單股斷裂。此外針對細胞之基因表現部分,MCF-7細胞曝露在東大以及草屯採樣站脂溶性樣本萃取物下,其p53蛋白有明顯上升;曝露於三個採樣站之脂溶性樣本則會造成CYP1A1代謝酵素明顯增加。綜合本研究之結果可知,空氣懸浮微粒的水溶性萃取物質,可以造成人類乳癌細胞胞內氧化壓力上升及基因單股斷裂。而脂溶性萃取物質則會造成MCF-7細胞p53以及CYP1A1基因大量表現,進而造成細胞凋亡以及胞內氧化壓力之生成。
URI: http://hdl.handle.net/11455/5505
其他識別: U0005-2508200814241100
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