請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/5681
標題: 除氮系統中與無氧氨氧化菌共存之微生物組成分析
Microbial Community Analysis of the Microorganisms Coexist with the Anammox Bacteria in the Biological Nitrogen Removal System
作者: 張婷婷
Chang, Ting-Ting
關鍵字: Anaerobic ammonia oxidation reaction
Anaerobic ammonia oxidation bacteria
Molecular biotechnology
出版社: 環境工程學系所
引用: 一、中文文獻 (一)書籍: 楊盛行 (1994) 農業與環境保育,華香園出版社,台北。 (二)期刊: 阮國棟、林郁真、吳婉怡及郭家成 (2005) 亞硝酸自營菌脫氮技術發展趨勢,全球化及近未來(Near Future)科技對環境管理之影響-環保署科顧室年度自行研究計畫論文集。 洪仁陽、張王冠、邵信和張敏超 (2001) Bionet高級生物處理技術應用,環保月刊,第1期:第182-192頁。 馮宇柔、曾四恭、夏聰惠、何俊明、闕士涵和陳志祥 (2006) 無氧氨氧化微生物於廢水除氮之研究,第三十一屆廢水處理技術研討會論文集,第173頁,台中。 謝淵琳、蔡慧穎、張裕釧及林畢修平 (2004) 本土性含氨氮無氧氧化微生物之混合族群在廢水除氮上的應用,中華民國環境保護協會學刊,第2期:第218-231頁。 (三)碩士論文: 石秉鑫 (2001) 以固定之好氧脫硝菌應用於同槽硝化脫硝反應之可行性研究,國立臺灣大學,碩士論文,台北。 李永文 (2003) 好氧式堆肥化處理之硝化菌分離與類源分析,國立中興大學,碩士論文,台中。 陳彥男 (2002) 三段式流體化床生物程序處理壓可力纖維製成廢水之程序研 究,碩士論文,國立成功大學,台南。 二、英文文獻 Achenbach, H., and Gottschalk, G. (1976) Acetate Metabolism in Rhodopseudomonas Gelatinosa and Several Other Rhodospirillaceae. Archives of Microbiology 111: 45-49. Ahn, Y.-H. (2006) Sustainable Nitrogen Elimination Biotechnologies: A Review. Process Biochemistry 41: 1709-1721. Amann, R.I., Binder, B.J., Olson, R.J., Chisholm, S.W., Devereux, R., and Stahl, D.A. (1990) Combination of 16s Rrna-Targeted Oligonucleotide Probes with Flow Cytometry for Analyzing Mixed Microbial Populations. Applied and Environmental Microbiology 56: 1919-1925. Anne, W.N., Wong, R.C.K., Chu, A., Bentley, L.R., and Lunn, S.R.D. (2004) Degradation of Monoethanolamine in Soil. Journal of Environmental Engineering and Science 3: 137-145. Anthonisen, A., Loehr, R., Prakasam, T., and Srinath, E. (1976) Inhibition of Nitrification by Ammonia and Nitrous Acid. Journal of Water Pollution Control Federation 48: 835-852. Arora, M.L., Barth, E.F., and Umphres, M.B. (1985) Technology Evaluation of Sequencing Batch Reactors. J. water pollutant control 57: 867-876. Bock, E., Schmidt, I., Stüven, R., and Zart, D. (1995) Nitrogen Loss Caused by Denitrifying Nitrosomonas Cells Using Ammonium or Hydrogen as Electron Donors and Nitrite as Electron Acceptor. Archives of Microbiology 163: 16-20. Boiran, B., Couton, Y., and Germon, J.C. (1996) Nitrification and Denitrification of Liquid Lagoon Piggery Waste in a Biofilm Infiltration-Percolation Aerated System (Bipas) Reactor. Bioresource Technology 55: 63-77. Castignetti, D., and Hollocher, T.C. (1984) Heterotrophic Nitrification among Denitrifiers. Applied and Environmental Microbiology 47: 620-623. Chae, K.-J., Rameshwar, T., Jang, A., Kim, S.H., and Kim, I.S. (2008) Analysis of the Nitrifying Bacterial Community in Biocube Sponge Media Using Fluorescent in Situ Hybridization (Fish) and Microelectrodes. Journal of Environmental Management 88: 1426-1435. Chamchoi , N., and Nitisoravut, S. (2007) Anammox Enrichment from Different Conventional Sludges. Chemosphere 66: 2225-2232. Chamchoi, N., Nitisoravut, S., and Schmidt, J.E. (2008) Inactivation of Anammox Communities under Concurrent Operation of Anaerobic Ammonium Oxidation (Anammox) and Denitrification. Bioresource Technology 99: 3331-3336. Chuang, H.-P., Yamaguchi, T., Harada, H., and Ohashi, A. (2008) Anoxic Ammonium Oxidation by Application of a Down-Flow Hanging Sponge(DHS) Reactor. Journal of Environmental Engineering and Management 6:409-417. Ciudad, G., Rubilar, O., Munoz, P., Ruiz, G., Chamy, R., Vergara, C., and Jeison, D. (2005) Partial Nitrification of High Ammonia Concentration Wastewater as a Part of a Shortcut Biological Nitrogen Removal Process. Process Biochemistry 40: 1715-1719. Daims, H., Bruhl, A., Amann, R., Schleifer, K.H., and Wagner, M. (1999) The Domain-Specific Probe Eub338 Is Insufficient for the Detection of All Bacteria: Development and Evaluation of a More Comprehensive Probe Set. . Systematic and Applied Microbiology 22: 434-444. Dapena-Mora, A., Campos, J.L., Mosquera-Corral, A., Jetten, M.S.M., and Mendez, R. (2004) Stability of the Anammox Process in a Gas-Lift Reactor and a Sbr. Journal of Biotechnology 110: 159-170. Dong, Z., and Sun, T. (2007) A Potential New Process for Improving Nitrogen Removal in Constructed Wetlands-Promoting Coexistence of Partial-Nitrification and Anammox. Ecological Engineering 31: 69-78. Eddy, M. (1991) Wastewater Engineering Treatment, Disposal, and Reuse. McGraw-Hill.New York Egli, K., Fanger, U., Alvarez, P.J.J., Siegrist, H., van der Meer, J.R., and Zehnder, A.J.B. (2001) Enrichment and Characterization of an Anammox Bacterium from a Rotating Biological Contactor Treating Ammonium-Rich Leachate. Archives of Microbiology 175: 198-207. Ehrich, S., Behrens, B., Lebedeva, A., Ludwig, W., and Bock, E.J. (1995) A New Obligately Chemolithoautotrophic, Nitrite-Oxidizing Bacterium, Nitrospira Moscoviensis sp. nov. and its Phylogenetic Relationship . Archives of Microbiology 164: 16-23. Fahrbach, M., Kuever, J., Remesch, M., Huber, B.E., Kampfer, P., Dott, W., and Hollender, J. (2008) Steroidobacter Denitrificans Gen.nov.,sp.nov.,a Steroidal Hormone-Degrading Gammaproteobacterium. International Journal of Systematic and Evolutionary Microbiology 58: 2215-2223. Finlay, B.J., Span, A.S.W., and Harman, J.M.P. (1983) Nitrate Respiration in Primitive Eukaryotes. Nature 303: 333-336. Fujii, T., Sugino, H., Rouse, J.D., and Furukawa, K. (2002) Characterization of the Microbial Community in an Anaerobic Ammonium-Oxidizing Biofilm Cultured on a Nonwoven Biomass Carrier. Journal of Bioscience and Bioengineering 94: 412-418. Fux, C., Marchesi, V., Brunner, I., and Siegrist, H. (2004) Anaerobic Ammonium Oxidation of Ammonium-Rich Waste Streams in Fixed-Bed Reactors. Water Science and Technology 49: 77-82. Fux, C., Boehler, M., Huber, P., Brunner, I., and Siegrist, H. (2002) Biological Treatment of Ammonium-Rich Wastewater by Partial Nitritation and Subsequent Anaerobic Ammonium Oxidation (Anammox) in a Pilot Plant. Journal of Biotechnology 99: 295-306. Guisasola, A., Qurie, M., Vargas, M.d.M., Casas, C., and Baeza, J.A. (2009) Failure of an Enriched Nitrite-Dpao Population to Use Nitrate as an Electron Acceptor. Process Biochemistry 44: 689-695. Gupta, A.B. (1997) Thiosphaera Pantotropha: A Sulphur Bacterium Capable of Simultaneous Heterotrophic Nitrification and Aerobic Denitrification. Enzyme and Microbial Technology 21: 589-595. Guven, D., Dapena, A., Kartal, B., Schmid, M.C., Maas, B., van de Pas-Schoonen, K., Sozen, S., Mendez, R., Op den Camp, H.J.M., Jetten, M.S.M., Strous, M., and Schmidt, I. (2005) Propionate Oxidation by and Methanol Inhibition of Anaerobic Ammonium-Oxidizing Bacteria. Applied and Environmental Microbiology 71: 1066-1071. Head, I.M., Hiorns, W.D., Embley, T.M., McCarthy, A.J., and Saunders, J.R. (1993) The Phylogeny of Autotrophic Ammonia-Oxidizing Bacteria as Determined by Analysis of 16S Ribosomal Gene Sequences. Journal of General Microbiology 139:1147-1153. Hellinga, C., Schellen, A.A.J.C., Mulder, J.W., van Loosdrecht, M.C.M., and Heijnen, J.J. (1998) The Sharon Process: An Innovative Method for Nitrogen Removal from Ammonium-Rich Waste Water. Water Science and Technology 37: 135-142. Hong, Z., Hanaki, K., and Mastsuo, T. (1994) Greenhouse Gas-N2o Production During Denitrification in Wastewater Treatment. Water Science and Technology 39: 13-21. Hsia, T.H., Feng, Y.J., Ho, C.M., Chou, W.P., and Tseng, S.K. (2008) Pva-Alginate Immobilized Cells for Anaerobic Ammonium Oxidation (Anammox) Process. Journal of Industrial Microbiology and Biotechnology 35: 721-727. Hyman, M.R., Murton, I.B., and Arp, D.J. (1988) Interaction of Ammonia Monooxygenase from Nitrosomonas Europaea with Alkanes, Alkenes, and Alkynes. Applied and Environmental Microbiology 54: 3187-3190. Imajo, U., Tokutomi, T., and Furukawa, K. (2003) Granulation of Anammox Microorganisms in up-Flow Reactors. Water Science and Technology 49: 155-163. Innerebner, G., Insam, H., Franke-Whittle, I.H., and Wett, B. (2007) Identification of Anammox Bacteria in a Full-Scale Deammonification Plant Making Use of Anaerobic Ammonia Oxidation. Systematic and Applied Microbiology 30: 408-412. Itokawa, H., Hanaki, K., and Matsuo, T. (2001) Nitrous Oxide Production in High-Loading Biological Nitrogen Removal Process under Low Cod/N Ratio Condition. Water Research 35: 657-664. Jetten, M.S.M., Horn, S.J., and van Loosdrecht, M.C.M. (1997) Towards a More Sustainable Municipal Wastewater Treatment System. Water Science and Technology 35: 171-180. Jetten, M.S.M., Wagner, M., Fuerst, J., van Loosdrecht, M., Kuenen, G., and Strous, M. (2001) Microbiology and Application of the Anaerobic Ammonium Oxidation (`Anammox'') Process. Current Opinion in Biotechnology 12: 283-288. Jetten, M.S.M., Strous, M., van de Pas-Schoonen, K.T., Schalk, J., van Dongen, U.G.J.M., van de Graaf, A.A., Logemann, S., Muyzer, G., van Loosdrecht, M.C.M., and Kuenen, J.G. (1998) The Anaerobic Oxidation of Ammonium. FEMS Microbiology Reviews 22: 421-437. Jianlong, W., and Ning, Y. (2004) Partial Nitrification under Limited Dissolved Oxygen Conditions. Process Biochemistry 39: 1223-1229. Jianlong, W., and Jing, K. (2005) The Characteristics of Anaerobic Ammonium Oxidation (Anammox) by Granular Sludge from an Egsb Reactor. Process Biochemistry 40: 1973-1978. Jin, R.C., Hu, B.L., Zheng, P., Qaisar, M., Hu, A.H., and Islam, E. (2008) Quantitative Comparison of Stability of Anammox Process in Different Reactor Configurations. Bioresource Technology 99: 1603-1609. Joo, H.S., Hirai, M., and Shoda, M. (2005) Characteristics of Ammonium Removal by Heterotrophic Nitrification-Aerobic Denitrification by Alcaligenes Faecalis No. 4 . Journal of Bioscience and Bioengineering 100: 184-191. Kalmokoff, M., Lu, D., Whitford, M., and Teather, R. (1999) Evidence for Production of a New Lantibiotic (Butyrivibriocin Or79a) by the Ruminal Anaerobe Butyrivibrio Fibrisolvens Or79: Characterization of the Structural Gene Encoding Butyrivibriocin Or79a. . Applied and Environmental Microbiology 65: 2128-2135. Kartal, B., Niftrik, L.v., Rattray, J., Vossenberg, J.L.C.M.v.d., Schmid, M.C., Damste, J.S., Jetten, M.S.M., and Strous, M. (2007a) Candidatus ''Brocadia Fulgida'':An Autofluorescent Anaerobic Ammonium Oxidizing Bacterium. FEMS Microbiology Ecology 63: 46-55. Kartal, B., Rattray, J., van Niftrik, L.A., van de Vossenberg, J., Schmid, M.C., Webb, R.I., Schouten, S., Fuerst, J.A., Damste, J.S., Jetten, M.S.M., and Strous, M. (2007b) Candidatus “Anammoxoglobus Propionicus” a New Propionate Oxidizing Species of Anaerobic Ammonium Oxidizing Bacteria Systematic and Applied Microbiology 30: 39-49. Khin, T., and Annachhatre, A.P. (2004) Novel Microbial Nitrogen Removal Processes. Biotechnology Advances 22: 519-532. Kindaichi, T., Ito, T., and Okabe, S. (2003) Ecophysiological Interaction between Nitrifying Bacteria and Heterotrophic Bacteria in Autotrophic Nitrifying Biofilms as Determined by Microautoradiography-Fluorescence in Situ Hybridization Applied and Environmental Microbiology 70: 1641-1650. Klemedtsson, L., Jiang, Q., Kasimir Klemedtsson, A., and Bakken, L. (1999) Autotrophic Ammonium-Oxidising Bacteria in Swedish Mor Humus. Soil Biology and Biochemistry 31: 839-847. Knowles, G., Downing, A.L., and Barrett, M.J. (1965) Determination of Kinetic Constants for Nitrifying Bacteria in Mixed Culture, with the Aid of an Electronic Computer. Journal of General Microbiology 38: 263. Kucera, I., Matyáek, R., and Dadák, V. (1986) The Influence of Ph on the Kinetics of Dissimilatory Nitrite Reduction in Paracoccus Denitrificans. Biochimica et Biophysica Acta (BBA) - Bioenergetics 848: 1-7. Kuypers, M.M., Sliekers, A.O., Lavik, G., Schmid, M., Jørgensen, B.B., Kuenen, J.G., Sinninghe, Damsté, J., S, Strous, M., and Jetten, M.S. (2003) Anaerobic Ammonium Oxidation by Anammox Bacteria in the Black Sea. Nature. 422: 608-611. Lee, Y.W., Ong, S.K., and Sato, C. (1997) Effects of Heavy Metals on Nitrifying Bacteria. . Water Science and Technology 36: 69-74. Lindsay, M., Webb, R., Strous, M., Jetten, M., Butler, M., Forde, R., and Fuerst, J. (2001) Cell Compartmentalisation in Planctomycetes: Novel Types of Structural Organisation for the Bacterial Cell. Archives of Microbiology 175: 413-429. Mancini, S.A., Devine, C.E., Elsner, M., Nandi, M.E., Ulrich, A.C., Edwards, E.A., and Sherwood Lollar, B. (2008) Isotopic Evidence Suggests Different Initial Reaction Mechanisms for Anaerobic Benzene Biodegradation. Environmental Science and Technology 42:8290-8296. Maness, P.C., Huang, J., Smolinski, S., Tek, V., and Vanzin, G. (2004) Energy Generation from the Co Oxidation-Hydrogen Production Pathway in Rubrivivax Gelatinosus. Applied and Environmental Microbiology 71: 2870-2874. Maszenan, A.M., Seviour, R.J., Patel, B.K.C., Janssen, P.H., and Wanner, J. (2005) Defluvicoccus vanus gen.nov.,sp.nov.,a Novel Gram-Negative Coccus/Coccobacillus in the ''Alphaproteobacteria'' from Activated Sludge. International Journal of Systematic and Evolutionary Microbiology 55: 2105-2111. McCartney, D.M., and Oleszkiewicz, J.A. (1993) Competition between Methanogens and Sulfate Reducers:Sulfate Ratio and Acclimation. Wat.Environ 65: 655-664. Mobarry, B.K., Wagner, M., Urbain, V., Rittmann, B.E., and Stahl, D.A. (1996) Phylogenetic Probes for Analyzing Abundance and Spatial Organization of Nitrifying Bacteria. Applied and Environmental Microbiology 62: 2156-2162. Mosier, A., Schimel, D., Valentine, D., Bronson, K., and Parton, W. (1991) Methane and Nitrous Oxide Fluxes in Native, Fertilized and Cultivated Grasslands. Nature 350: 330-332. Mulder, A., van de Graaf, A.A., Robertson, L.A., and Kuenen, J.G. (1995) Anaerobic Ammonium Oxidation Discovered in a Denitrifying Fluidized Bed Reactor. FEMS Microbiology Ecology 16: 177-183. Neef, A., Amann, R., Schlesner, H., and Schleifer, K.H. (1998) Monitoring a Widespread Bacterial Group: In Situ Detection of Planctomycetes with 16s Rrna-Targeted Probes. Microbiology 144: 3257-3266. Nielsen, A.T., Liu, W.-T., Filipe, C., Grady, L., Jr., Molin, S., and Stahl, D.A. (1999) Identification of a Novel Group of Bacteria in Sludge from a Deteriorated Biological Phosphorus Removal Reactor. Applied and Environmental Microbiology 65: 1251-1258. Noophan, P., Sripiboon, S., Damrongsri, M., and Munakata-Marr, J. (2009) Anaerobic Ammonium Oxidation by Nitrosomonas Spp. And Anammox Bacteria in a Sequencing Batch Reactor. Journal of Environmental Management 90: 967-972. Papen, H., von Berg, R., Hinkel, I., Thoene, B., and Rennenberg, H. (1989) Heterotrophic Nitrification by Alcaligenes Faecalis: No2-, No3-, N2o, and No Production in Exponentially Growing Cultures. Applied and Environmental Microbiology 55: 2068-2072. Randall, C.W. (1984) Nitrite Built-up in Activated Sludge Resulting from Temperature Effects. J. Water Pollutant Control 56: 1039-1044. ReginattoI, V., TeixeiraI, R.M., PereiraI, F., SchmidellI, W., JrI, A.F., MenesII, R., EtchebehereII, C., and SoaresI, H.M. (2005) Anaerobic Ammonium Oxidation in a Bioreactor Treating Slaughterhouse Wastewater. Brazilian Journal of Chemical Engineering 22: 593 - 600. Robertson, L.A., Van Niel, E.W.J., Torremans, R.A.M., and Kuenen, J.G. (1988) Simultaneous Nitrification and Denitrification in Aerobic Chemostat Cultures of Thiosphaera Pantotropha. Applied and Environmental Microbiology 54: 2812-2818. Rotthauwe, J.H., Witzel, K.P., and Liesack, W. (1997) The Ammonia Monooxygenase Structural Gene Amoa as a Functional Marker: Molecular Fine-Scale Analysis of Natural Ammonia-Oxidizing Populations. Applied and Environmental Microbiology 63: 4704-4712. Saha, P., Krishnamurthi, S., Mayilraj, S., Prasad, G.S., Bora, T.C., and Chakrabarti, T. (2005) Aquimonas Voraii Gen. Nov.,a Novel Gammaproteobacterium Isolated from a Warm Spring of Assam, India. International Journal of Systematic and Evolutionary Microbiology 55: 1491-1495. Sayed, S., de Zeeuw, W., and Lettinga, G. (1984) Anaerobic Treatment of Slaughterhouse Waste Using a Flocculant Sludge Uasb Reactor. Agricultural Wastes 11: 197-226. Schmid, M., Twachtmann, U., Klein, M., Strous, M., Juretschko, S., Jetten, M., Metzger, J.W., Schleifer, K.H., and Wagner, M. (2000) Molecular Evidence for Genus Level Diversity of Bacteria Capable of Catalyzing Anaerobic Ammonium Oxidation. Systematic and Applied Microbiology 23:93-106 Schmid, M., Walsh, K., Webb, R., Rijpstra, W.I., van de Pas-Schoonen, K., Verbruggen, M.J., Hill, T., Moffett, B., Fuerst, J., Schouten, S., Sinninghe Damste, J.S., Harris, J., Shaw, P., Jetten, M., and Strous, M. (2003) Candidatus "Scalindua Brodae", Sp. Nov., Candidatus "Scalindua Wagneri", Sp. Nov., Two New Species of Anaerobic Ammonium Oxidizing Bacteria. Systematic and Applied Microbiology 26: 529-538. Schmid, M.C., Maas, B., Dapena, A., van de Pas-Schoonen, K., van de Vossenberg, J., Kartal, B., van Niftrik, L., Schmidt, I., Cirpus, I., Kuenen, J.G., Wagner, M., Sinninghe Damste, J.S., Kuypers, M., Revsbech, N.P., Mendez, R., Jetten, M.S.M., and Strous, M. (2005) Biomarkers for in Situ Detection of Anaerobic Ammonium-Oxidizing (Anammox) Bacteria. Applied and Environmental Microbiology 71: 1677-1684. Schmidt, I., Sliekers, O., Schmid, M., Cirpus, I., Strous, M., Bock, E., Kuenen, J.G., and Jetten, M.S.M. (2002) Aerobic and Anaerobic Ammonia Oxidizing Bacteria - Competitors or Natural Partners? FEMS Microbiology Ecology 39: 175-181. Schmidt, I., Sliekers, O., Schmid, M., Bock, E., Fuerst, J., Kuenen, J.G., Jetten, M.S.M., and Strous, M. (2003) New Concepts of Microbial Treatment Processes for the Nitrogen Removal in Wastewater. FEMS Microbiology Reviews 27: 481-492. Sharma, B., and Ahlert, R.C. (1977) Nitrification and Nitrogen Removal. . Water Research 11: 897-925. Shuval, H.I., and Gruener, N. (1997) Infant Methemoglobinemia and Other Health Effects in Drinker Water. Prog. Wat. Tech: 183-193. Sinninghe Damste, J.S., Rijpstra, W.I.C., Geenevasen, J.A.J., Strous, M., and Jetten, M.S.M. (2005) Structural Identification of Ladderane and Other Membrane Lipids of Planctomycetes Capable of Anaerobic Ammonium Oxidation (Anammox). FEMS Microbiology Reviews 272: 4270-4283. Sliekers, A.O., Third, K.A., Abma, W., Kuenen, J.G., and Jetten, M.S.M. (2003) Canon and Anammox in a Gas-Lift Reactor. FEMS Microbiology Reviews 218: 339-344. Smith, P.G., and Coackley, P. (1984) Diffusivity, Tortuosity and Pore Structure of Activated Sludge. Water Research 18: 117-122. Stenstrom, M.K., and Poduska, R.A. (1980) The Effect of Dissolved Oxygen Concentration on Nitrification . Water Research 14: 643-649. Stephen, J.R., Kowalchuk, G.A., Bruns, M.-A.V., McCaig, A.E., Phillips, C.J., Embley, T.M., and Prosser, J.I. (1998) Analysis of Beta -Subgroup Proteobacterial Ammonia Oxidizer Populations in Soil by Denaturing Gradient Gel Electrophoresis Analysis and Hierarchical Phylogenetic Probing. Applied and Environmental Microbiology 64: 2958-2965. Stoutharmer, A.H. (1988) Dissimilatory Reduction of Oxidized Nitrogen Compounds. Biology of Anaerobic Microorganisms Zehnder,A.J.B.245-305. Stroo, H.F., Klein, T.M., and Alexander, M. (1986) Heterotrophic Nitrification in an Acid Forest Soil and by an Acid-Tolerant Fungus. Applied and Environmental Microbiology 52: 1107-1111. Strous, M., Kuenen, J.G., and Jetten, M.S. (1999) Key Physiology of Anaerobic Ammonium Oxidation. Applied and Environmental Microbiology 65: 3248-3250. Strous, M., Van Gerven, E., Kuenen, J.G., and Jetten, M. (1997a) Effects of Aerobic and Microaerobic Conditions on Anaerobic Ammonium-Oxidizing (Anammox) Sludge. Applied and Environmental Microbiology 63: 2446-2448. Strous, M., Heijnen, J.J., Kuenen, J.G., and Jetten, M.S.M. (1998) The Sequencing Batch Reactor as a Powerful Tool for the Study of Slowly Growing Anaerobic Ammonium-Oxidizing Microorganisms Applied Microbiology and Biotechnology 50: 589-596. Strous, M., Van Gerven, E., Zheng, P., Kuenen, J.G., and Jetten, M.S.M. (1997b) Ammonium Removal from Concentrated Waste Streams with the Anaerobic Ammonium Oxidation (Anammox) Process in Different Reactor Configurations. Water Research 31: 1955-1962. Sun, Y., Zuo, J., Chen, L., and Wang, Y. (2008) Eubacteria and Archaea Community of Simultaneous Methanogenesis and Denitrification Granular Sludge. Journal of Environmental Sciences 20: 626-631. Tal, Y., Watts, J.E.M., and Schreier, H.J. (2006) Anaerobic Ammonium-Oxidizing (Anammox) Bacteria and Associated Activity in Fixed-Film Biofilters of a Marine Recirculating Aquaculture System. Applied and Environmental Microbiology 72: 2896-2904. Tanaka, H., Uzman, S., and Dunn, I.J. (1981) Kinetics of Nitrification Using a Fluidized Sand Bed Reactor with Attached Growth. Biotechnology and Bioengineering 23: 1683-1702. Teske, A., Alm, E., Regan, J.M., Toze, S., Rittmann, B.E., and Stahl, D.A. (1994) Evolutionary Relationships among Ammonia- and Nitrite-Oxidizing Bacteria. Journal of Bacteriology 176: 6623-6630. Third, K.A., Sliekers, A.O., Kuenen, J.G., and Jetten, M.S.M. (2001) The Canon System (Completely Autotrophic Nitrogen-Removal over Nitrite) under Ammonium Limitation: Interaction and Competition between Three Groups of Bacteria. Systematic and Applied Microbiology 24: 588-596. Third, K.A., Paxman, J., Schmid, M., Strous, M., Jetten, M.S., and Cord-Ruwisch, R. (2005) Treatment of Nitrogen-Rich Wastewater Using Partial Nitrification and Anammox in the Canon Process. Water Science and Technology 52: 47-54. Tsushima, I., Kindaichi, T., and Okabe, S. (2007) Quantification of Anaerobic Ammonium-Oxidizing Bacteria in Enrichment Cultures by Real-Time Pcr. Water Research 41: 785-794. U.S.E.P.Agency (1993) Manual: Nitrogen Control. U.S. Environmental Protection Agency. Van de Graaf, A.A., De Bruijn, P., Robertson, L.A., Jetten, M.S.M., and Kuenen, J.G. (1996a) Autotrophic Growth of Anaerobic Ammonium-Oxidizing Micro-Organisms in a Fluidized Bed Reactor. Microbiology 142: 2187-2196 Van de Graaf, A.A., Bruijn, P., Robertson, L.A., Jetten, M.S.M., and Kuenen, J.G. (1996b) Autotrophic Growth of Anaerobic Ammonium-Oxidizing Micro-Organisms in a Fluidized Bed Reactor. Microbiology(UK) 142: 2187-2196. van Dongen, U., Jetten, M.S.M., and van Loosdrecht, M.C.M. (2001) The Sharon-Anammox Process for Treatment of Ammonium Rich Wastewater. Water Science and Technology 44 153–160. van Loosdrecht, M.C.M., and Jetten, M.S.M. (1997) Method of Treating Ammonia-Comprising Wastewater. van Niftrik, L.A., Fuerst, J.A., Damste, J.S.S., Kuenen, J.G., Jetten, M.S.M., and Strous, M. (2004) The Anammoxosome: An Intracytoplasmic Compartment in Anammox Bacteria. FEMS Microbiology Letters 233: 7-13. Vannelli, T., Logan, M., Arciero, D.M., and Hooper, A.B. (1990) Degradation of Halogenated Aliphatic Compounds by the Ammonia- Oxidizing Bacterium Nitrosomonas Europaea. Applied and Environmental Microbiology 56: 1169-1171. Verstraete, W., and Alexander, M. (1972) Heterotrophic Nitrification by Arthrobacter Sp. Journal of Bacteriology 110: 955-961. Woebken, D., Fuchs, B.M., Kuypers, M.M.M., and Amann, R. (2007) Potential Interactions of Particle-Associated Anammox Bacteria with Bacterial and Archaeal Partners in the Namibian Upwelling System. Applied and Environmental Microbiology 73: 4648-4657. Wolthoorn, A., Temminghoff, E.J.M., and van Riemsdijk, W.H. (2004) Effect of Synthetic Iron Colloids on the Microbiological Nh4+ Removal Process During Groundwater Purification. Water Research 38: 1884-1892. Wood, P.M. (1986) Nitrification as a Bacterial Energy Source. CSA. Wrage, N., Velthof, G.L., van Beusichem, M.L., and Oenema, O. (2001) Role of Nitrifier Denitrification in the Production of Nitrous Oxide. Soil Biology and Biochemistry 33: 1723-1732. Xiaodi, H., Joseph, J.H., and Mark, C.M.v.L. (2002) Sensitivity Analysis of a Biofilm Model Describing a One-Stage Completely Autotrophic Nitrogen Removal (Canon) Process. Biotechnology and Bioengineering 77: 266-277. Yoshie, S., Ogawa, T., Makino, H., Hirosawa, H., Tsuneda, S., and Hirata, A. (2006) Characteristics of Bacteria Showing High Denitrification Activity in Saline Wastewater. Letters in Applied Microbiology 42: 277-283. Zhanbei Liang, Rhae A. Drijber, Donald J. Lee, Ismail M. Dwiekat, Steven D. Harris, and Wedin, D.A. (2008) A Dgge-Cloning Method to Characterize Arbuscular Mycorrhizal Community Structure in Soil. Soil Biology & Biochemistry 40: 956-966. Zhu, J. (2000) A Review of Microbiology in Swine Manure Odor Control. Agriculture Ecosystems and Environment 78: 93-106. Zhu, J., and L.D.Jacobson (1999) Correlating Microbes to Major Odorous Compounds in Swine Manure. Journal of Environmental Quality 28: 737-744.
摘要: 無氧氨氧化反應(ANAMMOX,Anaerobic Ammonia Oxidation)為氮循環中較不明顯的分支,參與該反應之自營性無氧氨氧化菌特性為無氧環境中將氨氮與亞硝酸根直接反應生成氮氣,其化學方程式為NH4+ + NO2- → N2 + 2H2O,與傳統硝化、脫硝反應不同,此反應對於氧氣濃度較低的環境中,具有維持氮循環平衡的重要功能。本研究以工研院所設計之不同型式無氧氨氧化反應槽為研究對象,包括進流基質為氨氮與亞硝酸鹽之上流式厭氧污泥化系統(UASB)系統及進流基質為氨氮添加BioNET泡棉擔體之Partial nitrification/Anammox系統,利用聚合酶鏈鎖反應-變性梯度凝膠電泳(PCR-DGGE)、Cloning和螢光原位雜合(FISH)等分子生物技術探討不同型式無氧氨氧化系統之微生物菌群結構變化和除氮效能間的關係,此外亦探討與無氧氨氧化菌共存的微生物組成。 由微生物族群DGGE圖譜可發現,在UASB系統中確實存在著無氧氨氧化菌Candidatus Brocadia fulgida,同時系統中也存在著氨氧化菌ammonia-oxidizing Betaproteobacteria,推測氨氧化菌的作用在將氨氮轉換為亞硝酸鹽氮,並與無氧氨氧化菌Candidatus Brocadia fulgida共同將反應槽內之氨氮轉換為氮氣。此外,系統中亦存在有亞硝酸鹽氧化菌Nitrobacter sp.,且約占總菌數的5%左右,系統中亦存在有脫硝菌Steroidobacter denitrificans及梭狀芽孢桿菌Clostridium celatum、酸化菌Syntrophus sp.、Butyrivibrio fibrisolvens、硫酸還原菌Desulfobulbus sp.等。推測這些與生物除氮無直接關係的微生物扮演的角色可能為與有機質或蛋白質分解有關,進而釋放二氧化碳,提供給無氧氨氧化菌Candidatus Brocadia fulgida等自營菌所需之碳源,或可以消耗系統中的溶氧,提供無氧氨氧化菌適合生長的厭氧環境。 而添加BioNET泡棉擔體之Partial nitrification / Anammox系統則測定不到無氧氨氧化菌,污泥樣本中存在有氨氧化菌、亞硝酸鹽氧化菌Nitrospira sp.與Nitrobacter sp.、酸桿菌Bacterium Ellin、脫硝菌Defluvicoccus vanus、亞硝酸鹽氧化菌Nitrospira sp.、Uncultured bacterium、紫色不含硫菌Rubrivivax gelatinosus、革蘭氏陰性桿菌Aquimonas sp.與尚未定序的菌種等。 無氧氨氧化菌Candidatus Brocadia fulgida是目前為止發現唯一會自發螢光的無氧氨氧化菌,由於現今對於無氧氨氧化菌Candidatus Brocadia fulgida相關的結構與特性尚未釐清,且與其共生的微生物族群研究甚少,因此與無氧氨氧化菌Candidatus Brocadia fulgida共生的菌群間相互作用值得更進一步探討。
The anaerobic ammonia oxidation (ANAMMOX) reaction is one of side pathways in the nitrogen cycle. The anaerobic ammonia oxidation bacteria could directly oxidized ammonium and nitrite to nitrogen gas under anoxic conditions by the following equation NH4+ + NO2- → N2 + 2H2O. Anammox reaction, which is different from the traditional nitrification and denitrification reaction, is important for the balance of the nitrogen cycle under lower oxygen concentration environment. In this research, the microbial community structures of different type anaerobic ammonia oxidation reactors(developed by ITRI) including upflow anaerobic sludge bed (UASB) system and partial nitrification/Anammox system with BioNET cotton that operated under various influent concentrations were studied using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE)、Cloning and fluorescence in situ hybridization (FISH) techniques. Not only the relationship between the microbial community structures and nitrogen removal efficiency were compared, the possible roles of microorganisms co-existed with the anammox bacteria community were studied as well. Gene sequences obtained from DGGE patterns showed that anaerobic ammonia oxidation bacteria “Candidatus Brocadia fulgida” existed in the UASB system. Application of FISH quantification on both ammonia and nitrite oxidizing bacteria in the UASB indicated the existence of ammonia oxidizing β-proteobacteria(10%) and nitrite oxidizing bacteria Nitrobacter sp.(5%). We hypothesize that ammonia oxidizing bacteria converted ammonium to nitrite then being used by anammox bacteria to ensure total nitrogen removal. Results from DGGE experiments revealed that heterotrophic bacteria including Steroidobacter denitrificans、Clostridium celatum、Syntrophus sp.、Butyrivibrio fibrisolvens and Desulfobulbus sp. existed in the UASB system. We hypothesize that heterotrophic bacteria might be involved in the degradation of complex compounds in the UASB system to reduce the oxygen concentration and release carbon dioxide for aiding the survival of anammox bacteria. Ammonia oxidizing bacteria、nitrite oxidizing bacteria、Bacterium Ellin、Defluvicoccus vanus、Rubrivivax gelatinosus、Aquimonas sp. and certain uncultured bacterium existed in the Partial nitrification/Anammox with BioNET system. However, no anammox bacteria were detected. Candidatus Brocadia fulgida exhibited the common characteristics of anammox bacteria and its the biofilm aggregates could show strong autofluorescence. It is the only known anammox species exhibiting this feature. Detailed characteristics of this anammox bacterium remains unknown, so does research on the microbial community co-existed with Candidatus Brocadia fulgida. Further studies on Candidatus Brocadia fulgida as well as its interactions with the other microorganisms are needed.
URI: http://hdl.handle.net/11455/5681
其他識別: U0005-1507200923275500
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-1507200923275500


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