Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/5725
標題: 以生物添加法降解模擬含水層中四氯乙烯之砂箱試驗研究
Effects of Bioaugmentation on PCE-Contaminated Aquifer: A Sandbox Study
作者: 李孟熹
Lee, Meng-Hsi
關鍵字: tetrachloroethene
四氯乙烯
sandbox
M524
biostimulation
bioaugmentation
砂箱
M524
生物刺激
生物添加
出版社: 環境工程學系所
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摘要: 本研究藉由砂箱填充實場土壤,模擬四氯乙烯污染之飽和含水層,並以生物添加法植種具有完全脫氯能力之 M524 厭氧混合菌群,期望達成生物復育效果。 批次試驗顯示,現地菌於四十天內僅能將四氯乙烯脫氯降解至順-二氯乙烯,而 M524 於四氯乙烯降解過程中,無氯乙烯的累積即有乙烯的生成;此外,四氯乙烯與順-二氯乙烯還原脫氯皆為一階動力反應,但順-二氯乙烯脫氯速率(0.011 day-1)低於四氯乙稀(0.054 day-1),故順-二氯乙烯的代謝為四氯乙烯生物降解之速率限制步驟。 追蹤劑試驗顯示,含水層地下水流速(0.23 m/day)相較於實場偏快,使四氯乙烯的水動力延散以機械延散為主,而深層含水層水力傳導度較佳,使基質初始濃度較高,甲烷生成量較大,但隨流經距離的延長,可被利用基質驟減,造成上游端甲烷化情形較為明顯,至下游端甲烷生成速率便有減緩趨勢。 生物刺激階段,現地菌 Geobacter sp. 可將四氯乙烯脫氯成順-二氯乙烯, Comamonas testosteroni 亦可共代謝順-二氯乙烯,使四氯乙烯(3.9 mg/L)於含水層上游端即有 99 % 去除率,局部區域甚至低於地下水管制標準,且順-二氯乙烯生成曲線與甲烷生成曲線相似,顯示四氯乙烯的降解伴隨大量甲烷的生成與順-二氯乙烯的累積。生物添加階段,含水層有微量乙烯的產生,但降解副產物以順-二氯乙烯為主,因甲烷化反應顯著,現地菌 Sporotalea propionica 等能以氫氣為能源,故僅低比例之氫氣做為四氯乙烯還原性脫氯作用之電子供給者,顯示當外加菌適應現地環境後,應可使含水層具有完全脫氯能力。
URI: http://hdl.handle.net/11455/5725
其他識別: U0005-0308201011461000
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-0308201011461000
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