請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/5751
標題: 紫色不含硫光合作用細菌除磷能力與機制之探討
Phosphorus removal capacity and mechanism of purple nonsulfur (PNS) bacteria
作者: 梁志銘
Liang, Chih-Ming
關鍵字: purple nonsulfur (PNS) bacteria
紫色不含硫光合作用細菌
phosphorus removal
polyphosphate
除磷能力
聚磷酸鹽
出版社: 環境工程學系所
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摘要: 紫色不含硫光合作用細菌於已成功地被應用於廢水處理上,包含被使用於分解高濃度有機負荷之豬糞尿廢水、養殖廢水、難分解有機物及臭味去除等;近年來,更由於此類微生物所具有之生物產氫能力,而被廣泛地被應用於綠色能源開發之研究上。過去學者於利用紫色不含硫光合作用細菌進行生物產氫之過程中,意外地發現紫色不含硫光合作用細菌可能具有聚磷酸鹽累積能力,且過往文獻中對紫色不含硫光合作用細菌是否累積聚磷酸鹽之研究甚少,對其可能之累積途徑並未有所著墨,因此本研究將針對紫色不含硫光合作用細菌累積聚磷酸鹽之能力與代謝途徑進行探討。 本研究首先由活性污泥生物除磷系統中分離出數株紫色不含硫光合作用細菌,並由實驗證實各菌株所具有之磷酸鹽累積程度不盡相同,且發現菌株累積聚磷酸鹽之現象多發生在菌株成長至穩定生長期之時。 所分離出之紫色不含硫光合作用細菌純菌株於厭氧光照環境下可大量攝取環境中之磷酸鹽,進而以聚磷酸鹽顆粒之形式累積於菌體中,而所累積之聚磷酸鹽形式則以高分子量長鏈狀聚磷酸鹽為主,因此也使得此形式之聚磷酸鹽成為紫色不含硫光合作用細菌於環境能量不足時獲得能量之主要替代對象;此外,紫色不含硫光合作用細菌可於對數生長期時大量累積PHB,亦為少數被發現可同時累積聚磷酸鹽及PHB顆粒之微生物。而紫色不含硫光合作用細菌於好氧環境下則不利於菌體累積磷酸鹽,反之可能分解菌體中聚磷酸鹽顆粒而獲得能量,故導致釋放出磷酸鹽。研究中亦發現光照環境之提供有助於紫色不含硫光合作用細菌於厭氧環境下攝取磷酸鹽,並減少此類微生物於好氧環境下釋放磷酸鹽,顯示光源可能為紫色不含硫光合作用細菌累積聚磷酸鹽之重要因素。 本研究所推論出之紫色不含硫光合作用細菌聚磷酸鹽累積機制明顯與傳統認知之EBPR生物除磷模式有所不同。由於光照環境持續提供下,紫色不含硫光合作用細菌透過光合作用獲得ATP,而為了儲存過多的能量ATP,菌株持續表現酵素PPK,將能量ATP截斷後產生ADP,所截斷之正磷酸根則被紫色不含硫光合作用細菌轉以聚磷酸鹽形式儲存,而所產生之ADP則再度於光合作用反應裡合成ATP之用,故紫色不含硫光合作用細菌於穩定生長期時開始大量累積聚磷酸鹽顆粒。紫色不含硫光合作用細菌更可能是過往文獻中未被提及聚磷酸鹽累積菌;儘管本研究中所推論出之紫色不含硫光合作用細菌部分途徑與傳統EBPR生物聚磷酸鹽累積模式相似,但最大之不同點在於此類微生物累積聚磷酸鹽之能力可能為光驅動(light triggered)形式,並且可發生於厭氧環境條件下,為目前少數被證實於厭氧光照環境下可累積大量聚磷酸鹽之微生物。
Purple nonsulfur (PNS) bacteria have been successfully applied on wastewater treatment in recent decades. This microorganism has also been used for hydrogen production due to their biological characters recently. During the perious studies of hydrogen production using PNS bacteria, the intracellular polyphosphate was found in the PNS bacteria accidently. However, fewer studies focused on the phosphate removal capacity of PNS bacteria so far and the polyphosphate accumulation mechanism among this microorganism is unclear. Therefore, the aim of this study is to explore the phosphate removal capacity and to extablish the polyphosphate accumulation mechanism among PNS bacteria. Several PNS bacteria were firstly isolated from different activated sludges and their polyphosphate accumulation capacity had been demonstrated. Observiously, PNS bacteria generate intracellular polyphosphate under illuminative anaerobic condition when they grow into stationary phase. Under this condition, the isolated PNS bacteria up take the phosphate from environment and convert the phosphate into intracellular polyphosphate. High-molecular-weight polyphosphate (HPP) was the predominant phosphorus compound inside the PNS bacteria. Therefore, HPP was used for the alternative energy source by PNS bacteria when the environmental energy source was insufficient. Except polyphosphate, the PNS bacteria can also accumulate poly-β-hydroxybutyric acid (PHB). Fewer PNS bacteria were proofed that can generate both polyphosphate and PHB in the preious studies. Opposite to the illuminative anaerobic condition that the PNS bacteria can accumulate polyphosphate, aerobic condition was detrimental to the polyphosphate acuumulation capacity. The PNS bacteria decomposite the intraceulluar polyphosphate under aerobic dark condition and release the phosphate into environment. Furthermore, light was one of the main factors that advance the PNS bacteria generating or decomposting the intracellular polyphosphate. Overall, the mechanism and conditions of PNS bacteria acculumate polyphosphate are different to that of the well-known enhanced biological phosphorus removal (EBPR) system. For PNS bacteria, bacteria obtain ATP from performing photosynthesis. Part of obtained ATP were used for growth, the exceed ATP were convert by enzyme PPK into polyphosphate. Therefore, it is reasonably that the PNS bacteria generate polyphosphate when they grow into stationary phase. This study not only deomonstrated the PNS bacteria are one of the potential polyphosphate accumulating organisms that havn't been published before, but also are the microorgansism that can accumulate polyphosphate under anaerobic conditions.
URI: http://hdl.handle.net/11455/5751
其他識別: U0005-1708201011113100
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-1708201011113100
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