Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/5756
標題: 豬糞尿廢水處理系統結合植生復育之應用-不同水生植物與微生物之相互關係探討
An application of integrated phytoremediation/swine wastewater treatment system-exploring the relationships among aquatic plants and microorganisms
作者: 簡禎嫺
Chien, Chen-Hsien
關鍵字: swine wastewater
豬糞尿廢水
artificial floating island
rhizosphere effect
PCR-DGGE
amo gene
人工浮島
根圈效應
PCR-DGGE
amo gene
出版社: 環境工程學系所
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摘要: 養豬廢水具高濃度有機物與高營養鹽之特性,若未經妥善處理而直接排放至河川,將使大量污染物流入河川中,造成水源水質污染、優養化現象、魚貝類死亡、地下水污染等危害問題。目前大多數養豬場採用三段式廢水處理系統。近年來研究發現,雖然養豬場已普遍設置廢水處理設施,但由於養豬業者在缺乏專業知識與技術及設施逐漸老舊的情況下,導致處理效率不佳而超過放流水法規標準之問題。因此,研究開發出一套簡易操作、具經濟回收價值及有效污染減量的技術實為刻不容緩的課題。 本研究以植生處理法,將人工浮島放置於養豬場廢水處理之放流水水槽中,並選用觀賞植物等具經濟效益之作物,且植物的根及根圈有利於微生物分解及轉型污染物,藉此改善養豬廢水處理效率不佳之問題。採用批次式與連續式反應槽進行相關研究,批次式反應槽所選用之植物為雞冠花、羽竹;連續式反應槽所選用之植物為彩葉草、芳香萬壽菊。藉由PCR-DGGE分析此植生處理系統中之微生物族群,同時監測大腸桿菌群數量之變化並搭配水質分析,探討系統中微生物結構之變化情形。實驗結果顯示,於水質方面,批次式實驗中,植栽後第二週各反應槽之COD去除率即達到80%以上,且於植栽兩個月後,其COD均降至20~30mg/L,顯示此植生復育系統之COD去除效果佳。於微生物多樣性方面,以專一性引子對968f-GC/1392r針對微生物族群探討各反應槽之微生物族群多樣性,批次式實驗中,種植羽竹之反應槽的微生物多樣性較豐富,反之,種植雞冠花及未種植植物之反應槽的微生物多樣性隨時間改變而變得較單純,推測是由於植物根部能提供微生物生物膜生長所需之面積,雞冠花根部較短未生長至水面下以及未種植植物的情況下,無法提供水面下微生物生長所需的面積,可能是造成種植雞冠花及未種植植物之兩座反應槽微生物多樣性趨於單純的原因之一,另外,根據定序分析之比對結果,各反應槽水樣中均發現有Acidovorax sp.存在,且Acidovorax sp.可進行脫硝作用將硝酸鹽還原成亞硝酸鹽,因此,推測Acidovorax sp.在硝酸鹽氮的去除上具有貢獻。 連續式實驗中,因持續進流基質之優勢,且進流的同時會將進流水中之微生物帶入反應槽中,故微生物族群結構變化較小。彩葉草及芳香萬壽菊根部之微生物多樣性較羽竹根部之微生物多樣性豐富,族群數目較多,同時,彩葉草及芳香萬壽菊之根部較羽竹長且茂密,顯示較長之植物根部可有效保存系統中之微生物多樣性,符合批次式反應槽之分析結果。以專一性引子對amoA-1f / amoA-2r進行PCR反應,針對amo gene設計之引子對,證實系統中存在具有硝化能力之微生物,因此推測硝化作用產生之氫離子是使系統pH值下降原因之一。 本研究係利用分子生物技術分析系統中之微生物族群組成,瞭解不同植物與微生物之相互關係,進而探討植生處理系統對微生物族群組成造成之變化,評估此植生處理系統之可行性。
URI: http://hdl.handle.net/11455/5756
其他識別: U0005-1808201022590400
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-1808201022590400
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