Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/5850
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dc.contributor陳秀卿zh_TW
dc.contributorShiow-Ching Chenen_US
dc.contributor.author古家宇zh_TW
dc.contributor.authorKu, Chia-Yuen_US
dc.contributor.other環境工程學系所zh_TW
dc.date2012en_US
dc.date.accessioned2014-06-06T06:35:50Z-
dc.date.available2014-06-06T06:35:50Z-
dc.identifierU0005-2007201210005400en_US
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dc.identifier.urihttp://hdl.handle.net/11455/5850-
dc.description.abstract氫氣被認為是一項乾淨的能源,燃燒時只產生水與熱能(121 kJ/g),且燃燒後無排放二氧化碳之問題。而以紫色不含硫光合菌於光異營條件下進行生物產氫為具潛力的方式之一,但菌株生長速率較慢,以及光利用效率不佳之情形,且操作光合反應槽時,易被生長速率較快之異營菌污染,導致產氫速率與氫氣百分比下降。因此本研究以不同之操作策略(固定化細胞、光暗週期、共培養以及批次式進流方式),希冀能改善此缺點以提升產氫效能。另一方面,目前光合產氫多以鎢絲燈為光源,其發光時伴隨著大量熱能散失,且甚少文獻使用LED燈為光源。因此本研究仍嘗試以不同LED燈為光源,觀察對產氫及累積PHB之影響。 實驗結果發現,以固定化細胞方法進行光合產氫,固定化材質之質傳速率較差且阻礙光能穿透,因此光合菌無法利用光能進行產氫。以光暗週期方式進行光合產氫時,光暗週期之組別產氫量、細胞生長速率以及基質利用速率皆較全光照組別慢,並觀察到在黑暗期有耗氫情形發生。因此光暗週期操作之方式無法有效提升氫氣產量。將Rps. palustris WP3-5與藍綠細菌Anabaena sp. CH3以體積比1:1混合進行共培養批次試驗,glutamate濃度為50及100 mg/L時,累積氫氣量分別為43.1及45.8 ml H2。將光合反應槽操作於批次式進流,可成功延長反應槽操作時間至823(乙酸為電子供給者)及871(乳酸為電子供給者)小時並持續產氫,且受其他異營菌污染時,仍有高的氫氣百分比。 另外光合產氫批次試驗使用不同光源,結果發現以鎢絲燈為光源時,細胞生長速率、基質利用率以及產氫速率結果最快,若將光源改為LED燈,是以綠光LED燈為光源時,其細胞生長量及產氫量結果較多,但改變不同光源對於菌株WP3-5累積PHB並無影響,另外計算產生每莫耳氫氣所需之電能,若以綠光LED燈取代鎢絲燈時,可節省95%之電能。zh_TW
dc.description.abstractHydrogen is considered as an environmentally friendly fuel due to its carbon-neutral characteristic that only produces water and energy (121 kJ/g) after combustion. Using purple non-sulfur bacteria is a promising method to produce hydrogen biologically because this kind of bacteria can produce hydrogen gas under photo-heterotrophic condition. However, some problems associated with purple non-sulfur bacteria to produce hydrogen are still necessary to be solved, e.g., low cell growth rate, low conversion efficiency of light, and decreased hydrogen production rate and hydrogen content caused by heterotrophs which have high growth rate in photo-bioreactors. In this study, several strategies, including immobilized cell, diurnal-cycle operation, co-culture system and fed-batch mode operation, were tried to solve those problems which have been mentioned before and to enhance their hydrogen production. On the other hand, lots of studies used tungsten lamp as their light source for photo-hydrogen production, and it would waste high amount of energy when lighting on. Thus, second part of this study was to investigate the effect of LED light as a light source on photo-hydrogen production and PHB accumulation by Rhodopseudomoans palustris WP3-5. From the results, it can be concluded that (i) Rps. palustris strain WP3-5 can not successfully produce hydrogen in immobilized cells because of the limitation in light penetration, substrate transportation and waste exclusion; (ii) strain WP3-5 generated less hydrogen and lower biomass under a diurnal cycle operation than continuous illumination with a hydrogen consumption by uptake hydrogenase; (iii) batch experiments of co-culture hydrogen production by Rps. palustris WP3-5/Anabaena sp. CH3 under mixed ratio of 1/1 exhibited a maximum hydrogen volume of 45.8 and 43.1 ml when co-culture medium contained 50 and 100 mg/L glutamate, respectively; (iv) fed-batch mode photo-bioreactor using acetate and lactate as electron donor can continuously produce hydrogen until 823 and 871 hours, respectively, even if heterotrophs existed in the reactor. In addition, Rps. palustris WP3-5 had the highest cell growth rate, substrate consumption rate and hydrogen production rate when using tungsten lamp as a light source. Among various kinds of LED lamp for the other source, green-LED lamp had a higher hydrogen production rate than the other LED lamps. On the other hand, influence of light source on the PHB accumulation by strain WP3-5 was not observed. Otherwise, the energy demand to produce one mole of hydrogen gas by Rps. palustris WP3-5 was compared between the green-LED and tungsten lamps. The result showed that using green-LED lamp is of benefit to energy effiency by saving 95% of electric demand.en_US
dc.description.tableofcontents摘要 I Abstract II 目錄 IV 表目錄 VII 圖目錄 VIII 第一章 前言 1 第二章 文獻回顧 2 2.1 能源趨勢 2 2.2 替代能源分類 3 2.2.1 太陽能 3 2.2.2 風力能 3 2.2.3 地熱能 4 2.2.4 海洋能 4 2.2.5 水力能 4 2.2.6 生質能 4 2.3 微生物產氫系統 5 2.3.1 綠藻與藍綠細菌產氫 7 2.3.2 厭氧暗醱酵細菌產氫 8 2.3.3 光合作用細菌產氫 9 2.3.4 結合不同生物產氫系統產氫 9 2.4 紫色不含硫光合菌 10 2.4.1 產氫機制 12 2.4.2 影響因子 13 2.4.2.1 溫度 13 2.4.2.2 pH值 14 2.4.2.3 光強度 14 2.4.2.4 光波長 14 2.4.2.5 氣體組成 15 2.4.2.6 電子供給者 15 2.4.2.7 氮源 16 2.4.2.8 微量元素 16 2.4.2.9 胞內聚合物 16 2.5 光合產氫操作策略 16 2.5.1 以固定化細胞進行光合產氫 17 2.5.2 光暗週期進行光合產氫 17 2.5.3 藍綠菌與光合菌共培養系統 18 2.5.4 批次式進流進行光合產氫 18 2.6 紫色不含硫光合菌與發光二極體(LED) 18 2.6.1 發光二極體(LED)發光原理與特性 19 2.6.2 相關研究 19 2.7 紫色不含硫光合菌產氫與PHB累積之關係 20 2.7.1 PHAs之合成途徑 21 2.7.2 相關研究 22 第三章 材料與方法 25 3.1 實驗架構 25 3.2 菌種來源 26 3.2.1 紫色不含硫光合菌WP3-5與突變株M23 26 3.2.2 藍綠細菌 26 3.3 菌種保存及培養 27 3.3.1 紫色不含硫光合菌WP3-5與突變株M23保存及培養 27 3.3.2 藍綠細菌保存及培養 29 3.4 實驗方法 31 3.4.1 第一部分-Rhodopseudomonas palustris WP3-5延長光合產氫操作策略 31 3.4.1.1 固定化細胞批次試驗 31 3.4.1.2 光暗週期批次試驗 31 3.4.1.3 紫色不含硫光合菌與藍綠細菌共培養批次試驗 32 3.4.1.4 光合反應槽批次次進流產氫試驗 33 3.4.2 第二部分-不同LED燈對光合產氫與PHB累積之影響 34 3.4.2.1 批次試驗 34 3.5 突變株Rhodopseudomonas palustris M23 基因確認 35 3.5.1 Colony-PCR 條件設定 35 3.5.2 瓊膠電泳分析(agarose gel electrophoresis) 35 3.6 分析設備與方法 36 3.6.1 O.D.值 36 3.6.2 細胞乾重 36 3.6.3 pH值 36 3.6.4 產氣量 37 3.6.5 氣體組成 37 3.6.6 有機酸濃度 37 3.6.7 glutamate濃度 38 3.6.8 胞內PHAs 38 3.6.9 氨氮濃度 39 3.7 實驗用水與酸、鹼洗液 40 第四章 結果與討論 41 4.1 延長Rhodopseudomonas palustris WP3-5光合產氫操作策略 41 4.1.1 固定化細胞產氫批次試驗 41 4.1.2 光暗週期產氫批次試驗 44 4.1.3光合菌與藍綠菌共培養產氫批次試驗 50 4.1.3.1光強度為6000 lux之共培養批次試驗 50 4.1.3.2 光強度為3000 lux之共培養批次試驗 52 4.1.3.3 共培養培養基測試 53 4.1.3.4 光強度為3000 lux、glutamate濃度為100 mg/L之共培養產氫批次試驗 57 4.1.4 批次式進流光合產氫反應槽 60 4.1.4.1 乳酸為電子供給者產氫批次試驗 60 4.1.4.2 以乙酸為電子供給者之批次次進流光合產氫試驗 63 4.1.4.3 以乳酸為電子供給者之批次次進流光合產氫試驗 66 4.1.4.4 綜合討論 69 4.2 不同LED燈對光合產氫與PHB累積之影響 73 4.2.1 基因確認 73 4.2.2 以鎢絲燈為光源之產氫批次試驗 73 4.2.3 以暖白光LED燈為光源之產氫批次試驗 76 4.2.4 以白光LED燈為光源之產氫批次試驗 78 4.2.5 以紅光LED燈為光源之產氫批次試驗 81 4.2.6 以藍光LED燈為光源之產氫批次試驗 83 4.2.7 以綠光LED燈為光源之產氫批次試驗 86 4.2.8 綜合討論 88 第五章 結論與建議 96 5.1 結論 96 5.2 建議 96 參考文獻 98zh_TW
dc.language.isozh_TWen_US
dc.publisher環境工程學系所zh_TW
dc.relation.urihttp://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-2007201210005400en_US
dc.subjectRps. palustris WP3-5zh_TW
dc.subjectRps. palustris WP3-5en_US
dc.subject紫色不含硫光合菌zh_TW
dc.subjectLED燈zh_TW
dc.subjectpurple non-sulfur bacteriaen_US
dc.subjectLED lampen_US
dc.title延長Rhodopseudomonas palustris WP3-5光合產氫操作策略及LED燈對光合產氫與PHB累積之影響zh_TW
dc.titleStrategies to prolong photo-hydrogen production of Rhodopseudomonas palustris WP3-5 for long-term operation and effects of LED light on photo-hydrogen production and PHB accumulationen_US
dc.typeThesis and Dissertationzh_TW
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