Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/60317
標題: Characterization of a novel T4-type Stenotrophomonas maltophilia virulent phage Smp14
作者: Chen, C.R.
翁淑芬
Lin, C.H.
Lin, J.W.
Chang, C.I.
Tseng, Y.H.
Weng, S.F.
關鍵字: S. maltophilia
virulent phage
T4-type
unknown modified bases
major
capsid protein
phylogenetic analysis
xanthomonas-maltophilia
caulobacter-crescentus
escherichia-coli
bacteriophages
genome
identification
bacteremia
diversity
mechanism
evolution
期刊/報告no:: Archives of Microbiology, Volume 188, Issue 2, Page(s) 191-197.
摘要: Stenotrophomonas maltophilia (Sm), with most of the isolates being resistant to multidrugs, is an opportunistic bacterium causing nosocomial infections. In this study, a novel virulent Sm phage, Smp14, was characterized. Electron microscopy showed that Smp14 resembled members of Myoviridae and adsorbed to poles of the host cells during infection. It lysed 37 of 87 clinical Sm isolates in spot test, displayed a latent period of ca. 20 min, and had a burst size of ca. 150. Its genome (estimated to be 160 kb by PFGE), containing m4C and two unknown modified bases other than m5C and m6A as identified by HPLC, resisted to digestion with many restriction endonucleases except MseI. These properties indicate that it is a novel Sm phage distinct from the previously reported phiSMA5 which has a genome of 250 kb digestible with various restriction enzymes. Sequencing of a 16 kb region revealed 12 ORFs encoding structural proteins sharing 15-45% identities with the homologues from T4-type phages. SDS-PAGE displayed 20 virion proteins, with the most abundant one being the 39 kDa major capsid protein (gp23), which had the N-terminal 52 amino acids removed. Phylogenetic analysis based on gp23 classified Smp14 into a novel single-membered T4-type subgroup.
URI: http://hdl.handle.net/11455/60317
ISSN: 0302-8933
文章連結: http://dx.doi.org/10.1007/s00203-007-0238-5
Appears in Collections:分子生物學研究所

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