Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/60317
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dc.contributor.authorChen, C.R.en_US
dc.contributor.author翁淑芬zh_TW
dc.contributor.authorLin, C.H.en_US
dc.contributor.authorLin, J.W.en_US
dc.contributor.authorChang, C.I.en_US
dc.contributor.authorTseng, Y.H.en_US
dc.contributor.authorWeng, S.F.en_US
dc.date2007zh_TW
dc.date.accessioned2014-06-09T05:56:14Z-
dc.date.available2014-06-09T05:56:14Z-
dc.identifier.issn0302-8933zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/60317-
dc.description.abstractStenotrophomonas maltophilia (Sm), with most of the isolates being resistant to multidrugs, is an opportunistic bacterium causing nosocomial infections. In this study, a novel virulent Sm phage, Smp14, was characterized. Electron microscopy showed that Smp14 resembled members of Myoviridae and adsorbed to poles of the host cells during infection. It lysed 37 of 87 clinical Sm isolates in spot test, displayed a latent period of ca. 20 min, and had a burst size of ca. 150. Its genome (estimated to be 160 kb by PFGE), containing m4C and two unknown modified bases other than m5C and m6A as identified by HPLC, resisted to digestion with many restriction endonucleases except MseI. These properties indicate that it is a novel Sm phage distinct from the previously reported phiSMA5 which has a genome of 250 kb digestible with various restriction enzymes. Sequencing of a 16 kb region revealed 12 ORFs encoding structural proteins sharing 15-45% identities with the homologues from T4-type phages. SDS-PAGE displayed 20 virion proteins, with the most abundant one being the 39 kDa major capsid protein (gp23), which had the N-terminal 52 amino acids removed. Phylogenetic analysis based on gp23 classified Smp14 into a novel single-membered T4-type subgroup.en_US
dc.language.isoen_USzh_TW
dc.relationArchives of Microbiologyen_US
dc.relation.ispartofseriesArchives of Microbiology, Volume 188, Issue 2, Page(s) 191-197.en_US
dc.relation.urihttp://dx.doi.org/10.1007/s00203-007-0238-5en_US
dc.subjectS. maltophiliaen_US
dc.subjectvirulent phageen_US
dc.subjectT4-typeen_US
dc.subjectunknown modified basesen_US
dc.subjectmajoren_US
dc.subjectcapsid proteinen_US
dc.subjectphylogenetic analysisen_US
dc.subjectxanthomonas-maltophiliaen_US
dc.subjectcaulobacter-crescentusen_US
dc.subjectescherichia-colien_US
dc.subjectbacteriophagesen_US
dc.subjectgenomeen_US
dc.subjectidentificationen_US
dc.subjectbacteremiaen_US
dc.subjectdiversityen_US
dc.subjectmechanismen_US
dc.subjectevolutionen_US
dc.titleCharacterization of a novel T4-type Stenotrophomonas maltophilia virulent phage Smp14en_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1007/s00203-007-0238-5zh_TW
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