請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/60320
標題: Characteristic analysis of the luzA gene encoding chaperone from Photobacterium leiognathi related to bioluminescence
作者: Lin, J.W.
翁淑芬
Lin, B.J.
Chen, H.Y.
Weng, S.F.
關鍵字: acyl-protein synthetase
nucleotide-sequence
lux operon
bacterial
bioluminescence
escherichia-coli
functional-analysis
regulatory
region
vibrio-fischeri
expression
cloning
期刊/報告no:: Biochemical and Biophysical Research Communications, Volume 244, Issue 3, Page(s) 838-842.
摘要: Nucleotide sequence of the luzA gene (GenBank accession No. AF039303) from Photobacterium leiognathi ATCC 25521 (NCIMB 2193) has been determined, and the chaperone encoded by the luzA gene was deduced. The LuzA chaperone has a calculated M-r 26,295 and comprises 230 amino acid residues; the hydrophobic alpha-helix N-terminal 21 amino acid residues MKKTIF-ALLFMSVFI SYPSFA is the leader peptide, therefore the matured LuzA chaperone has a calculated M-r 23,871 and comprises 209 amino acid residues only. The periplasmic LuzA chaperone is the protein concerned with the protein folding, assembly and stability. The luzA gene and the related genes are closely linked to the sad gene, that encoding Cu/Zn superoxide dismutase enables to enhance bioluminescence of the lux operon; the gene order of the luzA gene and related genes is -ufo'-LuzA-ufoI-ufoII-ter->-R&R'-sod-ufo--->. In trans complementation bioluminoassays in vivo elicit that the LuzA chaperone might be not directly concerned with bioluminescence of the lux operon from P. leiognathi in E. coli, but might enable to stabilize the proteins related to bioluminescence. The unidentified ufoII gene closely linked to the luzA gene is able to enhance bioluminescence. (C) 1998 Academic Press.
URI: http://hdl.handle.net/11455/60320
ISSN: 0006-291X
文章連結: http://dx.doi.org/10.1006/bbrc.1998.8359
顯示於類別:分子生物學研究所

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