請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/60321
標題: Flagellin gene fliC of Xanthomonas campestris is upregulated by transcription factor Clp
作者: Lee, M.C.
翁淑芬
Weng, S.F.
Tseng, Y.H.
關鍵字: transcription factor Clp
flagellin
motility
virulence
phage phi-lf
pseudomonas-aeruginosa
escherichia-coli
receptor
protein
pv campestris
xanthan gum
activator
dna
expression
adhesion
期刊/報告no:: Biochemical and Biophysical Research Communications, Volume 307, Issue 3, Page(s) 647-652.
摘要: Clp, a homologue of cyclic AMP receptor protein (CRP), of Xanthomonas campestris regulates the expression of many genes. In this study, proteomic analysis showed the amounts of several extracellular proteins in a clp mutant to be reduced, including the flagellin encoded by fliC. Transcriptional fusion assay showed that activity of fliC promoter is reduced by 2.3-fold in clp mutant compared to the wild-type, coincident with the protein levels. The clp mutant is slightly reduced in motility; however, electron microscopy showed no significant change in the monotrichous flagellation. A fleQ homologue with conserved Clp-binding site in the upstream region is present in the fully sequenced X. campestris genome, suggesting that regulation of the flagellar genes is similar to that of Pseudomonas aeruginosa in involving Vfr, the CRP homologue, and FleQ in a cascade manner except that Vfr downregulates fleQ. Concomitant loss of flagellum and motility in fliC mutant and absence of a second homologue in the genome sequence suggest that X. campestris possesses a single flagellin gene, fliC. In addition, mutation of this gene does not affect virulence. (C) 2003 Elsevier Inc. All rights reserved.
URI: http://hdl.handle.net/11455/60321
ISSN: 0006-291X
文章連結: http://dx.doi.org/10.1016/s0006-291x(03)01223-3
顯示於類別:分子生物學研究所

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