Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/60329
DC FieldValueLanguage
dc.contributor.authorYang, T.C.en_US
dc.contributor.author翁淑芬zh_TW
dc.contributor.authorHu, R.M.en_US
dc.contributor.authorHsiao, Y.M.en_US
dc.contributor.authorWeng, S.F.en_US
dc.contributor.authorTseng, Y.H.en_US
dc.date2003zh_TW
dc.date.accessioned2014-06-09T05:56:15Z-
dc.date.available2014-06-09T05:56:15Z-
dc.identifier.issn1464-1801zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/60329-
dc.description.abstractXanthomonas campestris pv. campestris, which displays no significant beta-1,4-agalactopyranosidase activity, has three annotated beta-galactosidase genes in the sequenced genome, designated galA, galB and galC herein. GalA and GalB are similar to glycosyl hydrolase (GH) family 2 enzymes, including Escherichia coli LacZ. galA and galB cannot express detectable activity even after being cloned in-frame and driven by the vector's promoter. GalC is a GH35 enzyme homologous to the Xanthomonas axonopodis pv. manihotis Bga. The latter cleaves beta,1-3-linked galactose 1,000 times faster than beta,1-4-linked galactose and is not responsible for lactose utilization. In X campestris pv. campestris cells, GalC is readily detectable by Western blotting, and the levels can be increased by cloning the gene under the control of the vector's promoter. Results of insertional mutation, transcriptional fusion assay and Western blotting indicated that galC, clustered with several GH genes, is cotranscribed with the upstream gene(s) and is expressed constitutively. Xc17L is a previously isolated mutant with elevated beta-galactosidase activity and a greatly improved ability to grow on lactose. Results of DNA sequencing of Xc17L galA, galB and galC, enzyme assays of galA, galB and galC mutants derived from Xc17L, and Western blotting of GalC in Xc17L indicated that the three beta-galactosidase genes do not encode the elevated P-galactosidase activity in Xc17L. The presence of a fourth beta-galactosidase gene is proposed. Copyright (C) 2003 S. Karger AG, Basel.en_US
dc.language.isoen_USzh_TW
dc.relationJournal of Molecular Microbiology and Biotechnologyen_US
dc.relation.ispartofseriesJournal of Molecular Microbiology and Biotechnology, Volume 6, Issue 3-4, Page(s) 145-154.en_US
dc.relation.urihttp://dx.doi.org/10.1159/000077245en_US
dc.subjectbeta-galactosidaseen_US
dc.subjectXanthomonas campestrisen_US
dc.subjectglycosyl hydrolaseen_US
dc.subjectacid-sequence similaritiesen_US
dc.subjectphage-phi-lfen_US
dc.subjectxanthan gumen_US
dc.subjectglycosylen_US
dc.subjecthydrolasesen_US
dc.subjectstreptococcus-pneumoniaeen_US
dc.subjectcampestris strainen_US
dc.subjectgenomeen_US
dc.subjectsequenceen_US
dc.subjectlactoseen_US
dc.subjectcloningen_US
dc.subjectdnaen_US
dc.titleMolecular genetic analyses of potential beta-galactosidase genes in Xanthomonas camplestrisen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1159/000077245zh_TW
Appears in Collections:分子生物學研究所
文件中的檔案:

取得全文請前往華藝線上圖書館



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.