Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/60336
標題: Enantioselective synthesis of (S)-phenylephrine by whole cells of recombinant Escherichia coli expressing the amino alcohol dehydrogenase gene from Rhodococcus erythropolis BCRC 10909
作者: Lin, W.D.
許文輝
Chen, C.Y.
Chen, H.C.
Hsu, W.H.
關鍵字: Rhodococcus erythropolis
Amino alcohol dehydrogenase
Phenylephrine
Enantioselective synthesis
Bioconversion
short-chain dehydrogenases/reductases
stereoselective bioreduction
enantiomeric impurities
enzymatic-synthesis
chiral alcohols
reductase
phenylephrine
resolution
ketones
acid
期刊/報告no:: Process Biochemistry, Volume 45, Issue 9, Page(s) 1529-1536.
摘要: (R)-phenylephrine [(R)-PE] is an alpha(1)-adrenergic receptor agonist that is widely used in over-the-counter drugs to treat the common cold. We found that Rhodococcus erythropolis BCRC 10909 can convert detectable level of 1-(3-hydroxyphenyl)-2-(methylamino) ethanone (HPMAE) to (S)-PE by high performance liquid chromatography tandem mass spectrometry analysis. An amino alcohol dehydrogenase gene (RE_AADH) which possesses the ability to convert HPMAE to (S)-PE was then isolated from R. erythropolis BCRC 10909 and expressed in Escherichia coli NovaBlue. The purified RE_AADH, tagged with 6x His, had a molecular mass of approximately 30 kDa and exhibited a specific activity of 0.19 mu U/mg to HPMAE in the presence of NADPH, indicating this enzyme could be categorized as NADP(+)-dependent short-chain dehydrogenase reductase. E. coil NovaBlue cell expressing the RE_AADH gene was able to convert HPMAE to (S)-PE with more than 99% enantiomeric excess (ee), 78% yield and a productivity of 3.9 mmol (S)-PE/L h in 12 h at 30 degrees C and pH 7. The (S)-PE, recovered from reaction mixture by precipitation at pH 11.3, could be converted to (R)-PE (ee >99%) by Walden inversion reaction. This is the first reported biocatalytic process for the production of (S)-PE from HPMAE. (C) 2010 Elsevier Ltd. All rights reserved.
URI: http://hdl.handle.net/11455/60336
ISSN: 1359-5113
文章連結: http://dx.doi.org/10.1016/j.procbio.2010.06.003
Appears in Collections:分子生物學研究所

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