Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/60349
標題: Combining polymerase chain reaction restriction enzyme analysis with phenotypic characters for mycobacteria identification in Taiwan
作者: Shen, G.H.
陳建華
Hung, C.H.
Hu, S.T.
Wu, B.D.
Lin, C.F.
Chen, C.H.
Wu, K.M.
Chen, J.H.
關鍵字: BACTEC 960 MGIT system
rpoB duplex PCR-restriction enzyme analysis
(rpoB DPRA)
Mycobacterium tuberculosis complex
non-tuberculous
mycobacterium
fragment length polymorphism
tuberculosis complex
rapid
identification
nontuberculous mycobacteria
bactec cultures
gene rpob
system
probe
assay
differentiation
期刊/報告no:: International Journal of Tuberculosis and Lung Disease, Volume 13, Issue 4, Page(s) 472-479.
摘要: SETTING: Many hospitals use the fully-automated BACTEC 960 Mycobacteria Growth Indicator Tube (MGIT) system and acid-fast staining to detect acid-fast bacilli (AFB) in clinical specimens; however, labour intensive biochemical methods are used for further mycobacterial species identification. OBJECTIVE: To develop a user-friendly algorithm for mycobacterial species identification from AFB smear positive BACTEC tubes. DESIGN: AFB smear-positive BACTEC tubes were collected and mycobacteria were isolated and identified by biochemical methods. The tubes were subgrouped by rpoB duplex polymerase chain reaction restriction enzyme analysis (rpoB DPRA). The results were combined with key phenotypic characters of mycobacteria isolated from the tubes to develop a species identification algorithm with 16S rDNA sequencing of the isolate being used as the gold standard method. RESULTS: By rPoB DPRA, 441 AFB smear-positive BACTEC tubes were correctly subgrouped into 100 tubes containing Mycobacterium tuberculosis complex, 335 tubes containing non-tuberculous mycobacteria and six tubes containing both. A species identification algorithm was developed by combining the rpoB DPRA results of the tubes with growth rate, photoreactivity and two biochemical results of mycobacteria recovered from the tubes. CONCLUSION: This user-friendly algorithm can be used for mycobacterial species identification from AFB smear positive BACTEC tubes.
URI: http://hdl.handle.net/11455/60349
ISSN: 1027-3719
Appears in Collections:分子生物學研究所

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