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標題: Molecular epidemiology of Bordetella pertussis isolated in Taiwan, 1992-1997
作者: Lee, Y.S.
Yang, C.Y.
Lu, C.H.
Tseng, Y.H.
關鍵字: Bordetella pertussis
pulsed-field gel electrophoresis
field gel-electrophoresis
polymerase chain-reaction
期刊/報告no:: Microbiology and Immunology, Volume 47, Issue 12, Page(s) 903-909.
摘要: In Taiwan, the number of pertussis cases including various types of infection has been increasing in recent years, especially in 1997. Since 71% of the reported cases concentrated in the densely populated Taipei metropolitan area, concerns have been raised that a highly contagious strain of Bordetella pertussis might have appeared in Taipei. In this study, 114 strains of B. pertussis including those isolated in 1992-1996 (n=53) and 1997 (n=61) were subjected to pulsed-field gel electrophoresis (PFGE) of the XbaI digests from their chromosomes. Based on the band patterns, they were divided into 21 subtypes, P1 to P21. The strains isolated in 1997 consist of 17 subtypes including 9 new subtypes which did not appear in the previous years, indicating that the outbreaks in 1997 were not caused by a sole specific virulent strain. Dendrogram analysis indicated that the 21 subtypes can be grouped into five clusters, with the first four subtypes possessing 60 to 95% relatedness to one another, whereas relatedness between cluster 5 (containing P21 only) and the other clusters is less than 50%. Notably, all the subtypes except P12 and P21 appeared at least once in Taipei and the majority of the strains (54%) belong to two clusters, 3 and 4. These results suggest that highly dense population may facilitate spread and accelerate genetic divergence of this pathogen. This is the first report on pertussis molecular epidemiology in Taiwan.
ISSN: 0385-5600
Appears in Collections:分子生物學研究所



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