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標題: Immunoproteomic identification of the hypothetical protein NMB1468 as a novel lipoprotein ubiquitous in Neisseria meningitidis with vaccine potential
作者: Hsu, C.A.
Lin, W.R.
Li, J.C.
Liu, Y.L.
Tseng, Y.T.
Chang, C.M.
Lee, Y.S.
Yang, C.Y.
關鍵字: antigen
Neisseria meningitidis
membrane vesicle vaccine
serogroup-b meningococcus
confers protection
sequence variation
proteomic analysis
期刊/報告no:: Proteomics, Volume 8, Issue 10, Page(s) 2115-2125.
摘要: Many potential vaccine candidates for serogroup B Neisseria meningitidis (NMB) have been identified by reverse vaccinology, a genome-based approach. However, some candidates may be unseen owing to uncertain annotation or their peculiar properties. In this study, we describe the preparation and identification of a novel lipoprotein expressed in all meningococcal strains tested. mAb were first prepared from mice immunized with a meningococcal B strain isolated in Taiwan. Total proteins from the immunizing strain were separated by 2-DE in duplicate. Clone 4-7-3, which crossreacted to 174 tested meningococcal isolates, was used as the primary antibody for Western blotting. The immunoreactive spot was identified by LC-mass spectrometric analysis of the corresponding spot from the silver-stained gel and confirmed by molecular biology approach to be a novel lipoprotein encoded by the hypothetical NMB1468 gene. The potential use of this protein, designated Ag473/NMB1468, as a vaccine component was evaluated using the recombinant protein produced in Escherichia coli. Immunized mice were found to be protected from developing meningococcal disease after intraperitoneal inoculation with a lethal dose of meningococcal strain Nm22209, suggesting that Ag473/NMB1468 may be a promising vaccine candidate. This study also demonstrates the usefulness of the immunoproteomic approach in identificafion of novel vaccine candidates.
ISSN: 1615-9853
Appears in Collections:分子生物學研究所



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