Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/60416
標題: Avian reovirus sigma C protein induces apoptosis in cultured cells
作者: Shih, W.L.
劉宏仁
Hsu, H.W.
Liao, M.H.
Lee, L.H.
Liu, H.J.
關鍵字: avian reovirus
apoptosis
sigma C
immunofluorescent assay
flow
cytometric analysis
virus-induced apoptosis
attachment protein
infected-cells
gene-expression
in-vitro
kappa-b
pathway
death
identification
chickens
期刊/報告no:: Virology, Volume 321, Issue 1, Page(s) 65-74.
摘要: The avian reovirus (ARV) infection is associated with various disease conditions in poultry. However, the pathogenesis mechanisms are poorly characterized. In the present study, we clearly demonstrated that the sigmaC of ARV S1133 strain induced apoptosis in both BHK-21 and Vero cells. Five kinds of assays for apoptosis were used in analyzing ARV-infected BHK-21 and Vero cells: (1) assay for DNA ladders, (2) ELISA detection of cytoplasmic histone-associated DNA fragments, (3) nuclear staining with acridine orange, (4) Western blot, Northern blot, and immunofluorescent assay (IFA), and (5) flow cytometric analysis. The sigmaC protein of ARV could elicit apoptosis occurring in a dose- and time-dependent manner. The current results further our understanding of the function of sigmaC in cultured cells and suggest that sigmaC is a viral-encoded apoptin and possesses apoptosis-inducing ability. Furthermore, deletion analysis of the ARV sigmaC protein suggests that the carboxyl-terminus of sigmaC is important in mediating sigmaC-induced apoptosis because its deletion abolished the induction of apoptosis. (C) 2004 Elsevier Inc. All rights reserved.
URI: http://hdl.handle.net/11455/60416
ISSN: 0042-6822
文章連結: http://dx.doi.org/10.1016/j.virol.2003.12.004
Appears in Collections:分子生物學研究所

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