Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/60470
標題: Genomic cloning of 18 kDa oleosin and detection of triacylglycerols and oleosin isoforms in maturing rice and postgerminative seedlings
作者: Wu, L.S.H.
曾志正
Wang, L.D.
Chen, P.W.
Chen, L.J.
Tzen, J.T.C.
陳良築
關鍵字: aleurone layer
embryo
oil body
oleosin
rice
seed oil bodies
proteins
surface
identification
genes
期刊/報告no:: Journal of Biochemistry, Volume 123, Issue 3, Page(s) 386-391.
摘要: Oleosins are hydrophobic proteins localized abundantly in the oil bodies of plant seeds. Two distinct oleosin isoforms of molecular masses 18 and 16 kDa are present in rice oil bodies, These isoforms were found in similar ratio in rice embryos and aleurone layers, To survey potential DNA sequences involved in the activation of oleosin genes, a genomic clone of rice 18 kDa oleosin was sequenced, and its 5'-flanking region was compared with that of the known rice 16 kDa oleosin gene. Corresponding mRNAs of the two rice oleosin isoforms appeared seven days after pollination and vanished in mature seeds, Triacylglycerols and oleosins mere accumulated concomitantly in maturing rice reeds in accord with the active assembly of oil bodies, and partly mobilized in postgerminative seedlings, Approximately 60% of the stored triacylglycerols in rice were not utilized: while the majority of oil bodies in embryos were mobilized in five days after imbibition, those in aleurone layers remained intact in postgerminative seedlings.
URI: http://hdl.handle.net/11455/60470
ISSN: 0021-924X
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