Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/60477
標題: Characterization of interleukin-1 beta mRNA expression in chicken macrophages in response to avian reovirus
作者: Wu, Y.F.
邱繡河
Liu, H.J.
Shien, J.H.
Chiou, S.H.
Lee, L.H.
劉宏仁
關鍵字: double-stranded-rna
reverse transcription-pcr
eimeria-maxima
infection
bursal disease virus
toll-like receptor-3
protein-mu-ns
nf-kappa-b
phylogenetic analysis
mortality syndrome
poult enteritis
期刊/報告no:: Journal of General Virology, Volume 89, Page(s) 1059-1068.
摘要: Inhibitors of viral disassembly or RNA and protein synthesis, viral disassembly intermediates (infectious subviral particles, ISVP), binary ethylenimine-inactivated virions, and viral particles lacking genomic double-stranded (ds) RNA (empty particles) were used to assess the expression of interleukin-1 beta (IL-1 beta) mRNA in chicken (chIL-1 beta) macrophages in response to avian reovirus. The results demonstrate that two distinct expression patterns of chIL-1 beta mRNA mediated by different steps in viral replication were found. Viral disassembly was required for the induction of a rapid, transient expression pattern of chIL-1 beta mRNA that was rapidly induced at 30 min, with maximal levels reached by 2 h, and fell to a low level within 6 h post-inoculation, while viral RNA synthesis rather than protein translation, which was subsequent to membrane penetration, was required to induce a stable, sustained expression pattern of chIL-1 beta mRNA that occurred at and after 6 h post-inoculation. In addition, the induction of chIL-1 beta mRNA expression by the empty particles and ISVP was extremely weak, compared with the active dsRNA(+) virions or binary ethylenimine-inactivated virions, suggesting that the presence of dsRNA, even if transcriptionally inactive, may be an important factor in this response.
URI: http://hdl.handle.net/11455/60477
ISSN: 0022-1317
文章連結: http://dx.doi.org/10.1099/vir.0.82957-0
Appears in Collections:分子生物學研究所

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