Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/60481
標題: Development of a quantitative Light Cycler real-time RT-PCR for detection of avian reovirus
作者: Ke, G.M.
張天傑
Cheng, H.L.
Ke, L.Y.
Ji, W.T.
Chulu, J.L.C.
Liao, M.H.
Chang, T.J.
Liu, H.J.
劉宏仁
關鍵字: avian reovirus
Light Cyler
polymerase chain reaction
polymerase-chain-reaction
fragment length polymorphism
protein
sigma-ns
genome segment
phylogenetic analysis
binding activity
infected-cells
gene
identification
s1133
期刊/報告no:: Journal of Virological Methods, Volume 133, Issue 1, Page(s) 6-13.
摘要: A robust, ultrasensitive, and accurate quantitative assay was developed for avian reovirus (ARV) with the Light Cycler SYBR Green-based real-time reverse transcription-PCR (real-time LC RT-PCR). The assay exhibited high specificity as all negative controls and other avian pathogens, such as Newcastle disease virus (NDV), infectious bronchitis virus (IBV), infectious bursaL disease virus (IBDV), avian influenza virus (AIV), and mycoplasma synovia (NIS), failed to show any positive detection. A minimum of 39 copies/mu L of ARV genomic RNA could be detected by the assay. By dilution analysis, the real-time LC RT-PCR developed in this study was 3-log more sensitive than the conventional RT-PCR for the detection of ARV. The vaccine and field isolates of ARV were detected by the real-time LC RT-PCR. As a result of the high sensitivity and specificity of the assay with a relatively rapid and simple procedure, the real-time LC RT-PCR will be useful as a routine assay for the clinical diagnosis of ARV infection. (c) 2005 Elsevier B.V. All rights reserved.
URI: http://hdl.handle.net/11455/60481
ISSN: 0166-0934
文章連結: http://dx.doi.org/10.1016/j.jviromet.2005.09.011
Appears in Collections:分子生物學研究所

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