請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/60802
標題: Polycomb-Repressed Genes Have Permissive Enhancers that Initiate Reprogramming
作者: Taberlay, P.C.
劉俊吉
Kelly, T.K.
Liu, C.C.
You, J.S.
De Carvalho, D.D.
Miranda, T.B.
Zhou, X.J.
Liang, G.N.
Jones, P.A.
關鍵字: human genome
cell-lines
chromosome conformation
histone
modifications
chromatin signatures
myod transcription
target genes
stem-cells
cpg island
expression
期刊/報告no:: Cell, Volume 147, Issue 6, Page(s) 1283-1294.
摘要: Key regulatory genes, suppressed by Polycomb and H3K27me3, become active during normal differentiation and induced reprogramming. Using the well-characterized enhancer/promoter pair of MYOD1 as a model, we have identified a critical role for enhancers in reprogramming. We observed an unexpected nucleosome-depleted region (NDR) at the H3K4me1-enriched enhancer at which transcriptional regulators initially bind, leading to subsequent changes in the chromatin at the cognate promoter. Exogenous Myod1 activates its own transcription by binding first at the enhancer, leading to an NDR and transcription-permissive chromatin at the associated MYOD1 promoter. Exogenous OCT4 also binds first to the permissive MYOD1 enhancer but has a different effect on the cognate promoter, where the monovalent H3K27me3 marks are converted to the bivalent state characteristic of stem cells. Genome-wide, a high percentage of Polycomb targets are associated with putative enhancers in permissive states, suggesting that they may provide a widespread avenue for the initiation of cell-fate reprogramming.
URI: http://hdl.handle.net/11455/60802
ISSN: 0092-8674
文章連結: http://dx.doi.org/10.1016/j.cell.2011.10.040
顯示於類別:基因體暨生物資訊學研究所

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