Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/61708
標題: Silymarin: A Novel Antioxidant with Antiglycation and Antiinflammatory Properties In Vitro and In Vivo
作者: Wu, Chi-Hao
Huang, Shang-Ming
Yen, Gow-Chin
關鍵字: glycation end-products
naturally-occurring flavonoids
glucose-induced
expression
factor-kappa-b
diabetic-nephropathy
gene-transcription
monocytic cells
human-serum
dna-damage
histone h3
摘要: The current study was designed to evaluate the effects of silymarin (SM) on advanced glycation endproduct (AGE) formation and monocyte activation induced by S100b, a specific ligand of receptor for AGEs. The in vivo verification of antiglycation, antioxidant, and antiinflammatory capacities was examined by 12 weeks of SM administration in streptozotocin-diabetic rats. In vitro glycation assays demonstrated that SM exerted marked inhibition during the late stages of glycation and subsequent crosslinking. Dual action mechanisms, namely, antioxidant and reactive carbonyl trapping activities, may contribute to its antiglycation effect. SM produced a significant decrease in monocytic interleukin-1 beta and COX-2 levels and prevented oxidant formation caused by S100b, which appeared to be mediated by inhibition of p47phox membrane translocation. Chromatin immunoprecipitation demonstrated that S100b increased the recruitment of nuclear factor-kappaB transcription factor as well as cAMP response element-binding-binding protein and coactivator-associated arginine methyltransferase-1 cofactors to the interleukin-1 beta promoter, whereas these changes were inhibited with SM treatment. In vivo, SM reduced tissue AGE accumulation, tail collagen crosslinking, and concentrations of plasma glycated albumin. Levels of oxidative and inflammatory biomarkers were also significantly decreased in SM-treated groups compared with the diabetic group. These data suggest that SM supplementation may reduce the burden of AGEs in diabetics and may prevent resulting complications. Antioxid. Redox Signal. 14, 353-366.
URI: http://hdl.handle.net/11455/61708
ISSN: 1523-0864
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