Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/61718
標題: Glycine-N Methyltransferase Expression in HepG2 Cells Is Involved in Methyl Group Homeostasis by Regulating Transmethylation Kinetics and DNA Methylation
作者: Wang, Yi-Cheng
Tang, Feng-Yao
Chen, Shih-Yin
Chen, Yi-Ming
Chiang, En-Pei Isabel
關鍵字: performance liquid-chromatography
s-adenosylmethionine
rat-liver
rheumatoid-arthritis
dietary methionine
vitamin-b-6 status
folate-deficiency
plasma
homocysteine
metabolism
摘要: Glycine-N methyltransferase (GNMT) is a potential tumor suppressor that is commonly inactivated in human hepatoma. We systematically investigated how GNMT regulates methyl group kinetics and global DNA methylation. HepG2 cells (GNMT inactive, GNMT-) and cells transfected with GNMT expressed vector (GNMT+) were cultured in low (10 mu mol/L), adequate 1100 mu mol/L), or high (500 mu mol/L) L-methionine, each with 2.27 mu mol/L folate. Transmethylation kinetics were studied using stable isotopic tracers and GC-MS. Methylation status was determined by S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) levels, SAM:SAH ratio, DNA methyltransferase (DNMT) activity, and methylated cytidine levels in DNA. Compared with GNMT- cells, GNMT+ cells had lower homocysteine and greater cysteine concentrations. GNMT expression increased methionine clearance by inducing homocysteine transsulfuration and remethylation metabolic fluxes when cells were cultured in high or adequate L-methionine. In contrast, homocysteine remethylation flux was lower in GNMT+ cells than in GNMT- cells and homocysteine transsulfuration fluxes did not differ when cells were cultured in low methionine, suggesting that normal GNMT function helps to conserve methyl groups. Furthermore, GNMT expression decreased SAM and increased SAH levels and reduced DNMT activity in high or adequate, but not low, methionine cultures. In low methionine cultures, restoring GNMT in HepG2 cells did not lead to sarcosine synthesis, which would waste methyl groups. Methylated cytidine levels were significantly lower in GNMT- cells than in GNMT+ cells. In conclusion, we have shown that GNMT affects transmethylation kinetics and SAM synthesis and facilitates the conservation of methyl groups by limiting homocysteine remethylation fluxes. J. Nutr. 141: 777-782, 2011.
URI: http://hdl.handle.net/11455/61718
ISSN: 0022-3166
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