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標題: Production, optimization growth conditions and properties of the xylanase from Aspergillus carneus M34
作者: 方 繼
Fang, H.Y.
Chang, S.M.
Hsieh, M.C.
Fang, T.J.
關鍵字: Aspergillus carneus M34
response surface methodology (RSM)
agricultural wastes
statistical optimization
microbial xylanases
期刊/報告no:: Journal of Molecular Catalysis B-Enzymatic, Volume 49, Issue 1-4, Page(s) 36-42.
摘要: Growth conditions, including incubation times, temperature, agitation rate and initial pH of medium, that affect xylanase production by Aspergillus carneus M34 were studied sequentially use the classical '' change-one-factor-at-a-time '' method. Our results showed that there was a similar trend between cellular xylanase activity and extracellular xylanase activity. The optimal conditions for xylanase production, different from their cell growth, were on the third day, 30 degrees C, 100 rpm and pH 4, respectively, in this test. Response surface methodology (RSM) was further introduced to optimize the cultivation conditions and to evaluate the significance of these factors. The optimal cultivation conditions predicted from canonical analysis of this model were achieved by incubation at 35.08 degrees C with an agitation rate of 111.9 rpm and an initial pH of 5.16. In addition, temperature was the most critical factor for xylanase production by A. carneus M34. Xylanase activity of 22.2 U/mL was verified using the predicted optimal conditions and confirmed the fitness and applicability of the model. The optimal temperature and pH of the crude xylanase activity was observed at 60 degrees C and acidic pH, respectively. Sustained xylanase activity in the crude extract was also detected over a broad range of pH from 3 to 10. Considering its higher specificity toward agricultural wastes, especially corn cob and coba husk, this strain can be used to develop low-cost media for the mass-production of xylanase. (c) 2007 Elsevier B.V. All rights reserved.
ISSN: 1381-1177
Appears in Collections:食品暨應用生物科技學系



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