Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/61773
標題: Purification and characterization of a xylanase from Aspergillus carneus M34 and its potential use in photoprotectant preparation
作者: Fang, H.Y.
方 繼
Chang, S.M.
Lan, C.H.
Fang, T.J.
關鍵字: Aspergillus carneus
xylanase purification
characterization
feruloyl
xylooligosaccharide
antioxidative capacity
ferulic acid
wheat bran
oligosaccharides
endoxylanases
fermentation
growth
damage
fungi
straw
skin
期刊/報告no:: Process Biochemistry, Volume 43, Issue 1, Page(s) 49-55.
摘要: An extracellular xylanase was purified to homogeneity from a culture of Aspergillus carneus M34. In contrast to xylanases from other microorganisms, only a low-molecular weight xylanase, approximately 18.8 kDa with a pI value of 7.7-7.9, was purified in this investigation. The optimum temperature and pH of this purified xylanase activity were 50 degrees C and 6, respectively. The xylanase was more stable under alkaline conditions and retained more than 50% activity after 12 h incubation at pH 7-9. Considering of its characteristics and N-terminal sequence, this xylanase was concluded as a now one belonging to the group I of family 11 endoxylanases. In addition, hemicellulose of coba husk was selected as the substrate for xylooligosaccharide preparation owing to its higher specificity for this xylanase. Feruloyl xylooligosaccharides were separated and. shown potential antioxidative capacity in a cell model of ultraviolet B (UVB)-induced oxidative damage to keratinocyte xb-2. (c) 2007 Elsevier Ltd. All rights reserved.
URI: http://hdl.handle.net/11455/61773
ISSN: 1359-5113
文章連結: http://dx.doi.org/10.1016/j.procbio.2007.10.015
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