Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/61932
標題: FIP-fve Stimulates Interferon-Gamma Production via Modulation of Calcium Release and PKC-alpha Activation
作者: Ou, C.C.
林美吟
Hsiao, Y.M.
Wu, W.J.
Tasy, G.J.
Ko, J.L.
Lin, M.Y.
關鍵字: FIP
PKC-alpha
IFN-gamma
PBMC
calcium
protein-kinase-c
fungal immunomodulatory protein
blood
mononuclear-cells
nf-kappa-b
intracellular calcium
flammulina-velutipes
t-cells
transcription factors
cellular-responses
edible-mushroom
期刊/報告no:: Journal of Agricultural and Food Chemistry, Volume 57, Issue 22, Page(s) 11008-11013.
摘要: Fungal immunomodulatory protein, FIP-fve, has been isolated from Flammulina velutipes, and its immunomodulatory effects are believed to be associated with the enhanced activation of IFN-gamma-releasing Th1 cells. However, the mechanisms of FIP-fve-mediated signal transduction in the regulation of interferon-gamma (IFN-gamma) gene expression in human peripheral blood mononuclear cells (PBMCs) are still poorly understood. Using fluo-3 AM, we found that FIP-fve induces a rapid elevation in calcium concentration. ELISA, RT-PCR and Western blot assays demonstrated significant increases in the production and mRNA expression of IFN-gamma and protein kinase C-alpha (PKC-alpha) activation in activated PBMCs, which were abolished by EGTA, nifedipine and GO6976. In conclusion, Ca(2+) release and PKC-alpha activation are required for IFN-gamma production induced by FIP-fve in PBMCs.
URI: http://hdl.handle.net/11455/61932
ISSN: 0021-8561
文章連結: http://dx.doi.org/10.1021/jf902725s
Appears in Collections:食品暨應用生物科技學系

文件中的檔案:

取得全文請前往華藝線上圖書館



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.