請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/62025
標題: Enhancement of recombinant subtilisin YaB production by Bacillus subtilis
作者: Yeh, C.M.
葉娟美
Su, F.S.
Liao, Y.Y.
Tsai, Y.C.
關鍵字: artificial promoter
subtilisin YaB
extracellular production
Bacillus
subtilis
alkalophilic bacillus
rna-polymerase
nucleotide-sequence
promoter
sequences
alkaline elastase
secretion
gene
construction
sporulation
expression
期刊/報告no:: Food Biotechnology, Volume 21, Issue 1-2, Page(s) 105-117.
摘要: Bacillus subtilis is an ideal host for the production of extracellular heterologous proteins. The use of a strong, constitutive promoter is a good means of optimizing the expression of heterologous proteins. In this study, high level extracellular production of subtilisin YaB, a potent meat tenderizer, was achieved by engineering the wild-type subtilisin YaB promoter into an artificial promoter and expressed in Bacillus subtilis host. This artificial promoter provides an efficient tool to produce mutant subtilisin YaB or other recombinant heterologous proteins by B. subtilis at high level.
URI: http://hdl.handle.net/11455/62025
ISSN: 0890-5436
文章連結: http://dx.doi.org/10.1080/08905430701410506
顯示於類別:食品暨應用生物科技學系

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