請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/62027
標題: Extracellular production of a novel ice structuring protein by Bacillus subtilis - A case of recombinant food peptide additive production
作者: Yeh, C.M.
葉娟美
Wang, J.P.
Su, F.S.
關鍵字: ice structuring protein
extracellular production
Bacillus subtilis
antifreeze proteins
escherichia-coli
expression
gene
construction
secretion
meat
yab
期刊/報告no:: Food Biotechnology, Volume 21, Issue 1-2, Page(s) 119-128.
摘要: Bacillus subtilis is an ideal host for the production of extracellular heterologous proteins. Antifreeze protein (ice structuring protein, ISP) is a potent additive in cryogenic preservation of food products. In this investigation, the first production of a recombinant [SP was achieved by using a high-efficient artificial promoter and a signal peptide from subtilisin YaB. The functional ISP was expressed extracellularly at a high level by a protease deficient host, B. subtilis WB800. This study provides an efficient tool to produce recombinant food peptide additive at a high level and overcomes the problem of natural resources limitation as well.
URI: http://hdl.handle.net/11455/62027
ISSN: 0890-5436
文章連結: http://dx.doi.org/10.1080/08905430701410480
顯示於類別:食品暨應用生物科技學系

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