請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/62095
標題: Protective effect of pine (Pinus morrisonicola Hay.) needle on LDL oxidation and its anti-inflammatory action by modulation of NOS and COX-2 expression in LPS-stimulated RAW 264.7 macrophages
作者: Yen, G.C.
顏國欽
Duh, P.D.
Huang, D.W.
Hsu, C.L.
Fu, T.Y.C.
關鍵字: pine needle
LDL oxidation
inflammation
iNOS
COX-2
epicatechin
p-coumaric acid
low-density-lipoprotein
nitric-oxide production
nf-kappa-b
antioxidant activity
raw 264.7
cyclooxygenase-2 expression
free-radicals
oxidized-ldl
green tea
cells
期刊/報告no:: Food and Chemical Toxicology, Volume 46, Issue 1, Page(s) 175-185.
摘要: The protective effects of pine (Pinus morrisonicola Hay.) needle on low-density lipoprotein (LDL) oxidation and nitric oxide production in macrophages as well as its bioactive compounds were investigated. Of the four solvent extracts, the ethyl acetate extract of pine needle (EAE-PN) exhibited the strongest scavenging action on free radicals. EAE-PN significantly inhibited copper-induced LDL oxidation through prolonging the lag phase of conjugated dienes formation and decreasing the relative electrophoretic mobility of LDL. Lipid accumulation and foam cell formation were significantly reduced when EAE-PN (75 mu g/mL) was added to the medium co-incubated with macrophages cells and copper-induced LDL. EAE-PN also markedly inhibited reactive oxygen species production in RAW 264.7 cells stimulated with lipopolysaccharide (LPS). As regards NO production in cells, EAE-PN showed dose-dependent inhibitory effect on nitric oxide (NO) production in LPS-stimulated RAW 264.7 cells. The inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2) protein expressions in LPS-stimulated RAW 264.7 cells were inhibited by EAE-PN. RT-PCR analysis indicated that the iNOS and COX-2 mRNA expression were suppressed by EAE-PN. The major phenolic compounds in EAE-PN were epicatechin and p-coumaric acid by HPLC analysis. The presence of epicatechin and p-coumaric acid in EAE-PN may be partially responsible for the biological action of EAE-PN. Taken together, these results suggest that EAE-PN may provide potential protective effects against LDL oxidation and attenuating excessive NO generation at inflammatory sites; consequently, this may contribute to anti-atherosclerotic and anti-inflammatory effects of EAE-PN. (c) 2007 Elsevier Ltd. All rights reserved.
URI: http://hdl.handle.net/11455/62095
ISSN: 0278-6915
文章連結: http://dx.doi.org/10.1016/j.fct.2007.07.012
顯示於類別:食品暨應用生物科技學系

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