請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/62096
標題: The protective effects of Aspergillus candidus metabolites against hydrogen peroxide-induced oxidative damage to Int 407 cells
作者: Yen, G.C.
顏國欽
Chiang, H.C.
Wu, C.H.
Yeh, C.T.
關鍵字: Aspergillus candidus
3,3 ''-Dihydroxyterphenyllin
3-Hydroxyterphenyllin
Int 407 cells
oxidative damage
antioxidant activity
broth filtrate
comet assay
glutathione
extracts
stress
ldh
期刊/報告no:: Food and Chemical Toxicology, Volume 41, Issue 11, Page(s) 1561-1567.
摘要: The protective effects of 3,3&quot;-di-OH-terphenyllin (3,3&quot;-DHT) and 3-OH-terphenyllin (3-HT), two secondary metabolites produced by Aspergillus candidus (CCRC31543), against hydrogen peroxide-induced oxidative damage to Int 407 cells, were evaluated. The results showed that H2O2 caused an increase in lactate dehydrogenase (LDH) leakage and DNA damage in Int 407 cells; however, the addition of 3,3&quot;-DHT and 3-HT significantly reduced this effect (P < 0.05). Intracellular reactive oxygen species (ROS) formation in Int 407 cells pre-incubated with 3,3&quot;-DHT and 3-HT was decreased by 30 and 35%, respectively. The activity of glutathione peroxidase and catalase in Int 407 cells pre-incubated with 3,3&quot;-DHT was increased 25 and 33%, respectively; however, the activity of catalase was increased 30% in Int 407 cells pre-incubated with 3-HT. The activity of glutathione reductase in Int 407 cells pre-incubated with 3,3&quot;-DHT and 3-HT was decreased 36 and 21%, respectively. The intracellular glutathione level did not vary (P > 0.05), but oxidized glutathione levels increased when Int 407 cells were pre-incubated with these two compounds. These findings suggest that 3,3&quot;-DHT and 3-HT have the ability to protect against oxidative damage to Int 407 cells and that this protective effect may be associated with the ability to reduce ROS formation and increase catalase activity. (C) 2003 Elsevier Ltd. All rights reserved.
URI: http://hdl.handle.net/11455/62096
ISSN: 0278-6915
文章連結: http://dx.doi.org/10.1016/s0278-6915(03)00174-1
顯示於類別:食品暨應用生物科技學系

文件中的檔案:
沒有與此文件相關的檔案。


在 DSpace 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。