請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/62104
標題: Scavenging effects of lotus seed extracts on reactive nitrogen species
作者: Yen, G.C.
顏國欽
Duh, P.D.
Su, H.J.
Yeh, C.T.
Wu, C.H.
關鍵字: lotus seed
nitric oxide
peroxynitrite
single cell gel
electrophoresis
macrophage RAW 264.7
nitric-oxide production
line raw 264.7
tyrosine nitration
chlorogenic
acid
plant-extracts
dna-damage
in-vitro
peroxynitrite
inhibition
cells
期刊/報告no:: Food Chemistry, Volume 94, Issue 4, Page(s) 596-602.
摘要: The inhibitory effect of lotus (Nelumbo nicifera) seed extracts extracted with water (LSWE), ethyl acetate (LSEAE) and hexane (LSHE) on reactive nitrogen species, induced by DNA damage in macrophage RAW 264.7 cells, was investigated. The results showed that all extracts could inhibit nitric oxide accumulation in LPS-activated RAW 264.7 cells. The extracts in the range of 0.01-0.2 mg/ml showed a dose-dependent inhibitory effect on the accumulation of nitric oxide upon decomposition of sodium nitroprusside (SNP). The potency of inhibitory activity was in the order: LSEAE > LSWE > LSHE. The results of the comet assay indicated that the three extracts could inhibit DNA damage in macrophage RAW 264.7 cells induced by SNP. In addition, the three samples, at 0.2 mg/ml, showed 63%, 59%, and 38% inhibition of DNA damage in macrophage RAW 264.7 cells induced by peroxynitrite, respectively. All extracts tested were found to be potent peroxynitrite scavengers, capable of preventing the nitration of tyrosine. The data obtained suggest that lotus seed extracts might act as chemopreventers through reduction of excess amounts of nitric oxide. (c) 2005 Elsevier Ltd. All rights reserved.
URI: http://hdl.handle.net/11455/62104
ISSN: 0308-8146
文章連結: http://dx.doi.org/10.1016/j.foodchem.2004.11.052
顯示於類別:食品暨應用生物科技學系

文件中的檔案:
沒有與此文件相關的檔案。


在 DSpace 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。