請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/62118
標題: Anti-invasive Effect of a Rare Mushroom, Ganoderma colossum, on Human Hepatoma Cells
作者: Weng, C.J.
顏國欽
Fang, P.S.
Chen, D.H.
Chen, K.D.
Yen, G.C.
關鍵字: Ganoderma colossum
hepatocellular carcinoma
invasion
MAPK pathway
MMP-9 activity
signal-transduction pathway
nf-kappa-b
cancer cells
c-jun
lucidum
expression
tsugae
activation
binding
matrix-metalloproteinase-9
期刊/報告no:: Journal of Agricultural and Food Chemistry, Volume 58, Issue 13, Page(s) 7657-7663.
摘要: Ganoderma colossum is a rare species of the Ganodermataceae family with biological activity but has not been widely used to date. Because of its rareness and hard availability, the literature regarding the bioactivity of G. colossum is very limited and the bioactive components in G. colossum have never been explored. In the present study, an ethanol extract was prepared from the fruiting body of a G. colossum strain (EEGC) and then fractionated by reverese-phase high-performance liquid chromatography (HPLC). The inhibitory effects and molecular mechanisms of the EEGC on the phorbol-12-myristate-13-acetate (PMA)-induced invasion of HepG2 cells were investigated. The fractions of the EEGC cannot be totally identified, but the lucidenic acids were considered as the major component. When HepG2 cells were treated with the EEGC, the PMA-induced invasion was reduced in a dose-dependent manner and the PMA-induced matrix metalloproteinase (MMP)-9 was also suppressed at the transcriptional level. The EEGC also showed an inhibitory effect on the PMA-induced phosphorylation of extracellular signal-regulated kinase (ERK1/2) and protein kinase B (Akt) in cytosol, as well as the activator protein-1 (AP-1) and nuclear factor-kappa B (NF-kappa B) levels in the nucleus of HepG2 cells. This study provides the first evidence demonstrating that the EEGC is an effective inhibitior on the PMA-induced invasion of hepatoma cell. The EEGC could be further tested by an in vivo model to verify whether it is effective for the prevention of hepatoma invasion or metastasis.
URI: http://hdl.handle.net/11455/62118
ISSN: 0021-8561
文章連結: http://dx.doi.org/10.1021/jf101464h
顯示於類別:食品暨應用生物科技學系

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