Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/62147
標題: Low-Density-Lipoprotein (LDL)-Bound Flavonoids Increase the Resistance of LDL to Oxidation and Glycation under Pathophysiological Concentrations of Glucose in Vitro
作者: Wu, C.H.
顏國欽
Lin, J.A.
Hsieh, W.C.
Yen, G.C.
關鍵字: Advanced glycation endproducts
binging
flavonoids
glucose
glycation
LDL
oxidation
tocopherol
phenolic-compounds
tea polyphenols
vitamin-e
ex-vivo
plasma
humans
supplementation
glycoxidation
tocopherol
blood
期刊/報告no:: Journal of Agricultural and Food Chemistry, Volume 57, Issue 11, Page(s) 5058-5064.
摘要: The higher susceptibility of low-density lipoprotein (LDL) to oxidation and glycation in diabetes has been shown to be related to poor glycemic control. The aim of this study was to determine whether LDL-bound flavonoids attenuate high-glucose (HG)-mediated LDL oxidation and glycation. For this purpose, human plasma was preincubated with individual flavonoids for 3 h, followed by sequential ultracentrifugation and extensive dialysis to remove unbound flavonoid samples. Enriched LDL was subsequently isolated and challenged for its resistance to oxidation and glycation. Results showed that glucose (5-30 mM) dose-dependently accelerates copper (Cu(2+))-mediated LDL oxidative modification. The enrichment of flavonoids such as luteolin, naringenin, and kaempferol significantly increased the resistance of LDL to oxidation and prevented endogenous alpha-tocopherol consumption caused by HG/Cu(2+) (p < 0.05). The long-term glycation of LDL, which was measured by advanced glycation endproducts (AGEs)-related fluorescence and boronate affinity chromatography, was found to be inhibited by LDL-bound flavonoids in the following order: rutin > luteolin > quercetin > kaempferol > naringenin > catechin approximate to EC > naringin. Moreover, a solid-phase extraction system with HPLC-diode array detection provided evidence that flavonoids were bound to LDL particles to a certain extent concurrently facilitating the lipoprotein antioxidant and antiglycation activities. In conclusion, this study supports the hypothesis that HG promoted oxidative and glycative modifications of LDL. This is the first study to show that the introduction of flavonoids into LDL particles protects the lipoprotein against glycotoxin-mediated adverse effects.
URI: http://hdl.handle.net/11455/62147
ISSN: 0021-8561
文章連結: http://dx.doi.org/10.1021/jf9001445
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