Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/62155
標題: Suppression effect of soy isoflavones on nitric oxide production in RAW 264.7 macrophages
作者: Sheu, F.
顏國欽
Lai, H.H.
Yen, G.C.
關鍵字: nitric oxide
soy isoflavone
genistein
daidzein
glycitein
interferon-gamma
lipoprotein oxidation
synthase gene
in-vitro
induction
genistein
peroxynitrite
flavonoids
inhibitor
lipopolysaccharide
期刊/報告no:: Journal of Agricultural and Food Chemistry, Volume 49, Issue 4, Page(s) 1767-1772.
摘要: Genistein, daidzein, and glycitein, as primary isoflavones in soybeans, are reported to have beneficial effects on atherosclerosis, chronic inflammatory diseases, and cancers that are conducted by nitric oxide (NO) injury. The objectives of this study were to investigate the effects and mechanisms of these soy isoflavones on the inducible nitric oxide synthase (iNOS) system in lipopolysaccharide (LPS)-activated RAW 264.7 macrophages. Genistein, daidzein, and glycitein dose-dependently suppress NO production (IC50 = 50 CIM) in supernatants of LPS-activated macrophages as measured on the basis of nitrite accumulation. In addition, direct inhibition of iNOS activity, determined by means of the conversion of L-[H-3]arginine to L-[H-3]citrulline, and markedly reduced iNOS protein and mRNA levels, evaluated by means of Western blot and RT-PCR, respectively, were found in homogenates of LPS-activated cells treated with each isoflavone. Moreover, genistein was found to have a greater inhibitory effect on NO production but no significant effect on iNOS activity or protein and gene expression to daidzein and glycitein. These observations reveal that the suppression of NO production by genistein, daidzein, and glycitein might be due to the inhibition of both the activity and expression of iNOS in LPS-activated macrophages. The result suggests that soy isoflavones might attenuate excessive NO generation at inflammatory sites.
URI: http://hdl.handle.net/11455/62155
ISSN: 0021-8561
文章連結: http://dx.doi.org/10.1021/jf001198+
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