Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/65930
標題: 美洲含羞草相剋物質之研究
Allelochemicals of Mimosa diplotricha
作者: 陳信惟
Chen, Shin-Wei
關鍵字: Mimosa diplotricha
美洲含羞草
aggressive species
allelopathic activities
Ergosterol peroxide
Pheophorbide
外來入侵植物
相剋作用
過氧麥角固醇
脫鎂葉綠酸鹽
出版社: 森林學系所
引用: 石信德、黃振文 (2005) 生物農藥專題報導-保護植物的重要菌源鏈黴菌。科學發展391:22-27。 何政坤、張淑華、陳奎宏 (2005) 利用生物反應器生產二次代謝物。農業生技產業季刊 1:23-25。 吳志鴻 (2006) 林產研究新思維-抗氧化活性物質之利用潛能。林業研究專訊 13(4):1-4。 李士瑛、黃進發、廖啟成 (2000) 天然抗氧化素材GSH、SOD及Astaxanthin之開發與應用。生物產業 11:155-163。 張上鎮、王升陽 (1998) 來自台灣森林之芳香維他命。台灣工業24:33-37。 張上鎮、王升陽、吳季玲 (2000) 台灣杉木酚素之抗腫瘤潛力評估。中華林學季刊 32(2):277-282。 張上鎮、陳品方 (2000) 精油之抗細菌與抗真菌活性。林產工業19(2):275-284。 張喜寧 (2002) 蔬菜與水果的防癌性。科學農業 50:168-173。 郭佩旻 (2003) 紅檜心材抽出物之生物活性評估。碩士學位論文p:6-12 郭耀綸 (2001) 外來入侵種長穗木之個體生態學性狀及相剋作用潛力。台灣林業科學 16(2):103-114。 郭耀綸、陳志遠、林杰昌 (2002) 藉連續切蔓法及相剋作用防治外來入侵的小小花蔓澤蘭。台灣林業科學17(2):171-81。 劉業經、呂福原、歐辰雄 (1994) 台灣樹木誌p.206。 劉棠瑞、蘇鴻傑 (1983) 森林植物生態生理學。台灣商務印書館p.124-127。 蔣慕琰、徐玲明、袁秋英、陳富永、蔣永正 (2003) 小花蔓澤蘭危害與管理。研討會專刊p.97-109。 蔣慕琰、徐玲明 (2000) 外來植物在臺灣之野化、影響及管理。2000 年海峽兩岸生物多樣性與保育研討會論文集p.399-412。 蔡新聲、陳忠川、李鎮宇、郭昭麟、羅淑芳 (2003)藥用及保健植物專題報導-台灣珍稀藥用植物。科學發展364:42-49。 羅仁信 (2004) 大點雨杉的葉部化學成分研究。國立台灣大學研究所碩士論文p.22-25。 Bais, H.P., R. Vepachedu, S. Gilroy, R.M. Callaway and J.M. Vivanco (2003)Allelopathy and exotic plant invasion: from molecules and genes to species interactions. Science 301: 1377-1380. Beninger, C. W. and J. C. Hall (2005) Allelopathic activity of luteolin 7-Ο-β- glucuronide isolated from Chrysanthemum morifolium L. Biochemical Systematics and Ecology 33: 103-111. Bhowmik, P. C. and Inderjit (2003) Challenges and opportunities in implementing allelopathy for natural weed management. Crop Protection 22: 661-671. Czarnota, M., R. Paul, Dayan F., C. Nimbal and L. Weston (2001) Mode of action, localization of production, chemical nature, and activity of sorgoleone: a potential PS II inhibitor in Sorghum spp. root exudates. Weed Technology 15: 813-825. Cheng, H. H., H. K. Wang, J. Ito, K. F. Bastow, Y. Tachibana, Y. Nakanishi, Z. Xu, T. Y. Luo and K. H. Lee (2001) Cytotoxic Pheophorbide-Related Compounds from Clerodendrum calamitosum and C. cyrtophyllum. Journal of Natural Products 64: 915-919. Djurdjevic, L., A. Dinic, P. Pavlovic, M. Mitrovic, B. Karadzic and V. Tesevic (2004) Allelopathic potential of Allium ursinum L. Biochemical Systematics and Ecology 32: 533-544. Hierro, J. L. and R. M. Callaway (2003) Allelopathy and exotic plant invasion. Plant and Soil 256: 29-39. Issaq, H. J., K.C. Chan, G. M. Janini, T. P. Conrads and T. D. Veenstra (2005) Multidimensional separation of peptides for effective proteomic analysis. Journal of Chromatography B, 817: 35-47 James, R. V. (2002) Allelochemicals as leads for new herbicids and agrochemicals. Tetrahedron 58: 1631-1646. Jefferson, L.V. and M. Pennacchio (2003) Allelopathic effects of foliage extracts from four Chenopodiaceae species on seed germination. Journal of Arid Environments 55: 275-285. Kato-Noguchi, H. (2003) Allelopathic substances in Pueraria thunbergiana. Phytochemistry 63: 577-580. Kato-Noguchi, H. (2004) Allelopathic substance in rice root exudates: Rediscovery of momilactoneB as an allelochemical. Journal of Plant Physiology. 161: 271-276. Kato-Noguchi H. and Y. Tanaka (2004) Allelopathic potential of Citrus junos fruit waste from food processing industry. Bioresource Technology 94: 211-214. Kong, C., X. Xu, B. Zhou , F. Hu, C. Zhang and M. Zhang (2004) Two compounds from allelopathic rice accession and their inhibitory activity on weeds and fungal pathogens. Phytochemistry 65: 123-1128. Lee, C. H., M. H. Chin, Y. N. Wang and F. H. Chu (2006) Gene Investigation into the Inner bark of Taiwania (Taiwania cryptomerioides). Botanical Studies 47: 111-118. Leu, E., A. Kreiger-Liszkay, C. Goussias and E.M. Gross (2002) Polyphenolic allelochemicals from the aquatic angiosperm Myriophyllum spicatum inhibit photosystem II. Plant Physiol 130: 2011-2018. Li, F. M. and H. Y. Hu (2005) Isolation and Characterization of a Novel Antialgal Allelochemical from Phragmites communis. Applied and environmental microbiology: 6545-6553 Meazza, G., B. E. Scheffler, M. R. Tellez, A. M. Rimando, J. G. Romagni, S. O. Duke, D. Nanayakkara, I. A. Khan, E. A. Abourashed and F. E. Dayan (2002) The inhibitory activity of natural products on plant p-hydroxyphenylpyruvate dioxygenase. Phytochemistry 60: 281-288. Muscolo, A., M. R. Panuccio and M. Sidari (2001) The effect of phenols on respiratory enzymes in seed germination respiratory enzyme activities during germination of Pinus laricio seeds treated with phenols extracted from different forest soils. Plant Growth Regulation 35: 31-35. Nakajima, N., S. Hiradate and Y. Fuji (2001) Plant growth inhibitory activity of -canavanine and its mode of action. Journal of Chemical Ecology 27: 19-31. Oueslai, O. (2003) Allelopathy in two durum wheat (Triticum durum L.) varieties. Agriculture, Ecosystems and Environment 96:161-163. Rimando, A. M., F. E. Dayan, M. A. Czarnota, L. A. Weston and S. O. Duke (1998) A new photosystem II electron transport inhibitor from Sorghum bicolor. Journal of Natural Products 61: 927-930. Shao, H., S. Peng, X. Wei, D. Zhang and C. Zhang (2005)Potential allelochemicals from an invasive weed Mikania micrantha H.B.K. Journal of Chemical Ecology 31 (7): 1657-1668. Singh, H. P., D. R. Batish, N. Setia and R. K. Kohli (2005) Herbicidal activity of volatile oils from Eucalyptus citriodora against Parthenium hysterophorus. Annals of Applied Biology 146: 89-94. Vandermast, D.B., D.H. Van Lear and B.D. Clinton (2002) American chestnut as an allelopath in the southern Appalachians. Forest Ecology and Management 165: 173-181. Wang, J. F., Y. J. Huang, M. J. Fang, W. L. Xie, W. J. Su and Y. F. Zhao (2004) 5α,8α-Epidioxyergosta-6,22-dien-3β-ol (ergosterol peroxide) methanol solvate. Acta Crystallographica 60: 764-765. Weir, T. L., S. Park and J. M Vivanco (2004) Biochemical and physiological mechanisms mediated by allelochemicals. Current Opinion in Plant Biology 7: 472-479. Weir, T. L., H. Bais and J. M. Vivanco (2003) Intraspecific and interspecific interactions mediated by a phytotoxin, (S)-catechin, secreted by the roots of Centaurea maculosa (spotted knapweed). Journal of Chemical Ecology 29: 2397-2412. Wu, H., T. Haig, J. Pratley, D. Lemerle and M. An (2002) Biochemical basis for wheat seedling allelopathy on the suppression of annual Ryegrass (Lolium rigidum). Journal of Agricultural and Food Chemistry 50: 4567-4571. Yu, J. Q., S. F. Ye, M. F. Zhang and W. H. Hu (2003) Effects of root exudates and aqueous root extracts of cucumber (Cucumis sativus), and allelochemicals on photosynthesis and antioxidant enzymes in cucumber. Biochemical Systematics and Ecology 31: 129-139. 網路資源www.shu.ac.uk/schools/sci/chem/tutorials/chrom/gaschrm.htm Hostettmann, K. (1999) Strategy for the Biological and Chemical Evaluation of Plant Extracts. IUPAC URL: http://www.iupac.org/symposia/proceedings/phuket97/hostettmann.html Invasive Species Specialist Group (2003) The invasive species problems. http://www.issg.org/ James, J. F. and B. Rathinasabapathi (2003) Allelopathy: How Plants Suppress Other Plants. http://edis.ifas.ufl.edu/HS186 Macias, F. A., N. Chinchilla, R. M. Varela and J. M.G. Molinillo (2005) Bioactive compounds from Oryza sativa cv. Puntal Allelopathy Congress Powered by Regional. http://www.regional.org.au
摘要: 外來入侵植物美洲含羞草(Mimosa diplotricha =M. invisa )在台灣已經產生一些生態問題,雖然不如小花蔓澤蘭的惡名昭彰,但其擴張速率與範圍亦不容忽視。為了解其對立地競爭之可能機制,本試驗利用有機溶劑萃取法,用甲醇萃取出其植株地上部的抽出物,進行相剋作用的生物活性之評估。試驗結果顯示,美洲含羞草的粉末對於其他外來入侵植物大花咸豐草(Bidens pilosa)、紫花霍香薊(Ageratum houstonianum)的種子,及小白菜(Brassica chinensis)種子具有延遲發芽的效果。由生物試驗得知,濃度為1000 ppm時,抑制小白菜發芽的活性依序為乙酸乙酯可溶部>甲醇抽出物>乙酸乙酯不可溶部。本研究續利用管柱層析及薄層層析等技術,將乙酸乙酯可溶部劃分成許多分離部。其中有一分離部對小白菜種子有完全抑制發芽的效果,於該分離部鑑定出二個過氧麥角固醇類(Ergosterol peroxide),分別為5α,8α-Epidioxyergosta-6,22-dien-3β-ol與5α,8α-Epidioxyergosta-6-dien-3β-ol。從另一分離部萃取出的脫鎂葉綠酸鹽(Pheophorbide)對剛發芽的小白菜胚軸具有抑制活性。綜合本研究所得知結果,美洲含羞草之抽出成分具有多樣之相剋作用活性,值得未來繼續針對其所含活性成分之確切結構及其作用機制進一步的深入探討。
Mimosa diplotricha, an invasive species, shown some ecological problems in Taiwan. Although it wasn't notorious as Mikania micrantha H.B.K, it grown and spread so quickly that can't be ignored. In order to understand the mechanism of the competitiveness, we used extract of M. diplotricha by methanol, and assessed the bioactive. Result shown that the powder of M. diplotricha inhibited the germination and growth of Brassica chinensis, Bidens pilosa and Ageratum houstonianum. We tested the activity of extract at 1000 ppm and the order of allelopathic activities were EtOAc fraction > MeOH extract > EtOAc insolvable fraction. Screening for bioactive compounds and the EtOAc fraction subsequent isolation by chromatography separation. The extract inhibited germination of B. chinensis completely. Following by chromatography separation and spectral analysis, two steroids were identified, including ergosterol peroxide, 5α,8α-Epidi- oxyergosta-6,22- dien-3β-ol and ergosterol peroxide, 5α,8α- Epidioxyergosta-6-dien-3β-ol. And another extract (Pheophorbide) were identified, it inhibited the radical growth of seedling. According to the results, the extractives of M. diplotricha possess multiple bioactivities. It is worthy to investigate the active mechanisms and the exact structures of active components in the future.
URI: http://hdl.handle.net/11455/65930
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-2808200716382000
Appears in Collections:森林學系

文件中的檔案:

取得全文請前往華藝線上圖書館

Show full item record
 
TAIR Related Article
 
Citations:


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.