Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/66302
標題: 多殺型巴斯德桿菌脂多醣體抗原合成基因之研究與快速分類之應用
Characterization of The Biosynthesis Genes for Lipopolysaccharide Somatic Antigens of Pasteurella multocida and Application to Rapid-typing
作者: 蔡雨蓁
Tsai, Yu-Chen
關鍵字: Pasteurella multocida
多殺型巴斯德桿菌
lipopolysaccharide
somatic antigen
biosynthesis gene
typing
脂多醣
體抗原
合成基因
分類
出版社: 微生物暨公共衛生學研究所
引用: 呂榮修, 蔡向榮, 曾俊憲, 林地發, 1994, 台灣禽畜敗血性出血性巴氏桿菌之血清型及生化學特性. 台灣省畜衛所研報 30, 7. Adler, B., Chancellor, R., Homchampa, P., Hunt, M., Ruffolo, C., Strugnell, R., Wapling, D., 1996, Immunity and vaccine development in Pasteurella multocida infections. J Biotechnol 44, 139-144. Blocker, D., Berod, L., Fluhr, J.W., Orth, J., Idzko, M., Aktories, K., Norgauer, J., 2006, Pasteurella multocida toxin (PMT) activates RhoGTPases, induces actin polymerization and inhibits migration of human dendritic cells, but does not influence macropinocytosis. Int Immunol 18, 459-464. Bowersock, T.L., Hooper, T., Pottenger, R., 1992, Use of ELISA to detect toxigenic Pasteurella multocida in atrophic rhinitis in swine. J Vet Diagn Invest 4, 419-422. Boyce, J.D., Adler, B., 2000, The capsule is a virulence determinant in the pathogenesis of Pasteurella multocida M1404 (B:2). Infect Immun 68, 3463-3468. Boyce, J.D., Harper, M., St Michael, F., John, M., Aubry, A., Parnas, H., Logan, S.M., Wilkie, I.W., Ford, M., Cox, A.D., Adler, B., 2009, Identification of novel glycosyltransferases required for assembly of the Pasteurella multocida A:1 lipopolysaccharide and their involvement in virulence. Infect Immun 77, 1532-1542. Carter, G., 1955, Studies on Pasteurella multocida I. A haemagglutination test for the identification of serological types. American Journal of Veterinary Research 16, 4. Carter, G., 1963, Proposed midification of the Serological Classification of Pasteurella multocida. Veterinary Research 75, 1. Carter, G.a.D.A., MCL, 1989, Haemorrhagic Septicaemia. In: Pasteurella and Pasteurellosis. Academic Press Limited, London, 131-160 pp. Carter, G.R., 1961, A new serological type of Pasteurella multocida from Central Africa. Veterinary Research 73, 1. Chanter, N.a.R., JM, 1989, Pasteurellosis in Pigs and the Determinants of Virulence of Toxigenic Pasteurella multocida. In: Pasteurella and Pasteurellosis. Academic Press, London, 161-195 pp. Christensen, J.P., Bisgaard, M., 2000, Fowl cholera. Rev Sci Tech 19, 626-637. Corney, B.G., Diallo, I.S., Wright, L.L., Hewitson, G.R., De Jong, A.J., Burrell, P.C., Duffy, P.F., Stephens, C.P., Rodwell, B.J., Boyle, D.B., Blackall, P.J., 2007, Pasteurella multocida detection by 5'' Taq nuclease assay: a new tool for use in diagnosing fowl cholera. J Microbiol Methods 69, 376-380. Cox, A.D., Zou, W., Gidney, M.A., Lacelle, S., Plested, J.S., Makepeace, K., Wright, J.C., Coull, P.A., Moxon, E.R., Richards, J.C., 2005, Candidacy of LPS-based glycoconjugates to prevent invasive meningococcal disease: developmental chemistry and investigation of immunological responses following immunization of mice and rabbits. Vaccine 23, 5045-5054. Dawkins, H.J., Johnson, R.B., Spencer, T.L., Patten, B.E., 1990, Rapid identification of Pasteurella multocida organisms responsible for haemorrhagic septicaemia using an enzyme-linked immunosorbent assay. Res Vet Sci 49, 261-267. De Alwis, M.C., 1992, Haemorrhagic septicaemia--a general review. Br Vet J 148, 99-112. Dziva, F., Muhairwa, A.P., Bisgaard, M., Christensen, H., 2008, Diagnostic and typing options for investigating diseases associated with Pasteurella multocida. Vet Microbiol 128, 1-22. Foged, N.T., 1988, Quantitation and purification of the Pasteurella multocida toxin by using monoclonal antibodies. Infect Immun 56, 1901-1906. Galdiero, M., Folgore, A., Nuzzo, I., Galdiero, E., 2000, Neutrophil adhesion and transmigration through bovine endothelial cells in vitro by protein H and LPS of Pasteurella multocida. Immunobiology 202, 226-238. Ganfield, D.J., Rebers, P.A., Heddleston, K.L., 1976, Immunogenic and toxic properties of a purified lipopolysaccharide-protein complex from Pasteurella multocida. Infect Immun 14, 990-999. Glorioso, J.C., Jones, G.W., Rush, H.G., Pentler, L.J., Darif, C.A., Coward, J.E., 1982, Adhesion of type A Pasteurella mulocida to rabbit pharyngeal cells and its possible role in rabbit respiratory tract infections. Infect Immun 35, 1103-1109. Hansen, L.M., Hirsh, D.C., 1989, Serum resistance is correlated with encapsulation of avian strains of Pasteurella multocida. Vet Microbiol 21, 177-184. Harper, M., Boyce, J.D., Cox, A.D., St Michael, F., Wilkie, I.W., Blackall, P.J., Adler, B., 2007a, Pasteurella multocida expresses two lipopolysaccharide glycoforms simultaneously, but only a single form is required for virulence: identification of two acceptor-specific heptosyl I transferases. Infect Immun 75, 3885-3893. Harper, M., Cox, A., St Michael, F., Parnas, H., Wilkie, I., Blackall, P.J., Adler, B., Boyce, J.D., 2007b, Decoration of Pasteurella multocida lipopolysaccharide with phosphocholine is important for virulence. J Bacteriol 189, 7384-7391. Harper, M., Cox, A.D., St Michael, F., Wilkie, I.W., Boyce, J.D., Adler, B., 2004, A heptosyltransferase mutant of Pasteurella multocida produces a truncated lipopolysaccharide structure and is attenuated in virulence. Infect Immun 72, 3436-3443. Heddleston K.L., J.E.G., and P.A. Rebers, 1972, Fowl cholera: gel diffusion precipitin test for serotyping Pasteurella multocida from avian species. Avian Diseases 16, 11. Horadagoda, N.U., Hodgson, J.C., Moon, G.M., Wijewardana, T.G., Eckersall, P.D., 2002, Development of a clinical syndrome resembling haemorrhagic septicaemia in the buffalo following intravenous inoculation of Pasteurella multocida serotype B:2 endotoxin and the role of tumour necrosis factor-alpha. Res Vet Sci 72, 194-200. Kamp, E.M., Bokken, G.C., Vermeulen, T.M., de Jong, M.F., Buys, H.E., Reek, F.H., Smits, M.A., 1996, A specific and sensitive PCR assay suitable for large-scale detection of toxigenic Pasteurella multocida in nasal and tonsillar swabs specimens of pigs. J Vet Diagn Invest 8, 304-309. KL Heddleston, J.G., and PA Rebers, 1972, Fowl cholera: gel diffusion precipitin test for serotyping Pasteurella multocida from avian species. Avian Dis 16, 11. Lax, A.J., Chanter, N., 1990, Cloning of the toxin gene from Pasteurella multocida and its role in atrophic rhinitis. J Gen Microbiol 136, 81-87. Liu, D., Lawrence, M.L., Austin, F.W., 2004, Specific PCR identification of Pasteurella multocida based on putative transcriptional regulator genes. J Microbiol Methods 58, 263-267. Logan, S.M., Chen, W., Aubry, A., Gidney, M.A., Lacelle, S., St Michael, F., Kuolee, R., Higgins, M., Neufeld, S., Cox, A.D., 2006, Production of a D-glycero-D-manno-heptosyltransferase mutant of Mannheimia haemolytica displaying a veterinary pathogen specific conserved LPS structure; development and functionality of antibodies to this LPS structure. Vet Microbiol 116, 175-186. Marshall, M.S., Robison, R.A., Jensen, M.M., 1981, Use of an enzyme-linked immunosorbent assay to measure antibody responses in turkeys against Pasteurella multocida. Avian Diseases 25, 964-971. May, B.J., Zhang, Q., Li, L.L., Paustian, M.L., Whittam, T.S., Kapur, V., 2001, Complete genomic sequence of Pasteurella multocida, Pm70. Proc Natl Acad Sci U S A 98, 3460-3465. Mendes, S., Carmichael, K.P., Nunnally, J.C., Glisson, J.R., Cheng, I.H., Harmon, B.G., 1994, Lesions resulting from attempted Shwartzman reaction in turkey poults inoculated with Pasteurella multocida lipopolysaccharide. Avian Diseases 38, 790-796. Miflin, J.K., Blackall, P.J., 2001, Development of a 23S rRNA-based PCR assay for the identification of Pasteurella multocida. Lett Appl Microbiol 33, 216-221. Namioka, S., 1978, Pasteurella multocida—biochemical characteristics and serotypes. Methods in microbiology 10, 20. Namioka, S., and M. Murata, 1961, Serological studies on Pasteurella multocida.Ⅰ.A simplified method for capsule typing of the organism. National Institute of Animal Health, Kodaira, Tokyo, Japan 3, 9. P.J. Quinn, B.K.M., M.E. Carter, W.J. Donnelly, and F.C. Leonard, 2005, Veterinary Microbiology and Microbial Disease. Blackwell Publishing, 137-143 pp. Parker, C.T., Pradel, E., Schnaitman, C.A., 1992, Identification and sequences of the lipopolysaccharide core biosynthetic genes rfaQ, rfaP, and rfaG of Escherichia coli K-12. J Bacteriol 174, 930-934. Raetz, C.R., Whitfield, C., 2002, Lipopolysaccharide endotoxins. Annu Rev Biochem 71, 635-700. Ram, S., Cox, A.D., Wright, J.C., Vogel, U., Getzlaff, S., Boden, R., Li, J., Plested, J.S., Meri, S., Gulati, S., Stein, D.C., Richards, J.C., Moxon, E.R., Rice, P.A., 2003, Neisserial lipooligosaccharide is a target for complement component C4b. Inner core phosphoethanolamine residues define C4b linkage specificity. J Biol Chem 278, 50853-50862. Register, K.B., DeJong, K.D., 2006, Analytical verification of a multiplex PCR for identification of Bordetella bronchiseptica and Pasteurella multocida from swine. Vet Microbiol 117, 201-210. Rhoades, K.a.R., RB, 1989, Fowl Cholera. In: Pasteurella and Pasteurellosis. Academic Press Limited, London, 99-154 pp. Rhoades, K.R., Rimler, R.B., 1987a, Capsular groups of Pasteurella multocida isolated from avian hosts. Avian Diseases 31, 895-898. Rhoades, K.R., Rimler, R.B., 1987b, Effects of Pasteurella multocida endotoxins on turkey poults. Avian Diseases 31, 523-526. Rimler, R.a.R., KR, 1989, Pasteurella multocida. In: Pasteurella and Pasteurellosis. Academic Press Limited, London, 37-73 pp. Rimler, R.B., 1990, Comparisons of Pasteurella multocida lipopolysaccharides by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to determine relationship between group B and E hemorrhagic septicemia strains and serologically related group A strains. J Clin Microbiol 28, 654-659. Ruffolo, C.G., Tennent, J.M., Michalski, W.P., Adler, B., 1997, Identification, purification, and characterization of the type 4 fimbriae of Pasteurella multocida. Infect Immun 65, 339-343. St Michael, F., Harper, M., Parnas, H., John, M., Stupak, J., Vinogradov, E., Adler, B., Boyce, J.D., Cox, A.D., 2009, Structural and genetic basis for the serological differentiation of Pasteurella multocida Heddleston serotypes 2 and 5. J Bacteriol 191, 6950-6959. St Michael, F., Li, J., Cox, A.D., 2005a, Structural analysis of the core oligosaccharide from Pasteurella multocida strain X73. Carbohydr Res 340, 1253-1257. St Michael, F., Li, J., Vinogradov, E., Larocque, S., Harper, M., Cox, A.D., 2005b, Structural analysis of the lipopolysaccharide of Pasteurella multocida strain VP161: identification of both Kdo-P and Kdo-Kdo species in the lipopolysaccharide. Carbohydr Res 340, 59-68. St Michael, F., Vinogradov, E., Li, J., Cox, A.D., 2005c, Structural analysis of the lipopolysaccharide from Pasteurella multocida genome strain Pm70 and identification of the putative lipopolysaccharide glycosyltransferases. Glycobiology 15, 323-333. Swords, W.E., Buscher, B.A., Ver Steeg Ii, K., Preston, A., Nichols, W.A., Weiser, J.N., Gibson, B.W., Apicella, M.A., 2000, Non-typeable Haemophilus influenzae adhere to and invade human bronchial epithelial cells via an interaction of lipooligosaccharide with the PAF receptor. Mol Microbiol 37, 13-27. Swords, W.E., Ketterer, M.R., Shao, J., Campbell, C.A., Weiser, J.N., Apicella, M.A., 2001, Binding of the non-typeable Haemophilus influenzae lipooligosaccharide to the PAF receptor initiates host cell signalling. Cell Microbiol 3, 525-536. Townsend, K.M., Boyce, J.D., Chung, J.Y., Frost, A.J., Adler, B., 2001, Genetic organization of Pasteurella multocida cap Loci and development of a multiplex capsular PCR typing system. J Clin Microbiol 39, 924-929. Townsend, K.M., Frost, A.J., Lee, C.W., Papadimitriou, J.M., Dawkins, H.J., 1998, Development of PCR assays for species- and type-specific identification of Pasteurella multocida isolates. J Clin Microbiol 36, 1096-1100. Valvano, M.A., Marolda, C.L., Bittner, M., Glaskin-Clay, M., Simon, T.L., Klena, J.D., 2000, The rfaE gene from Escherichia coli encodes a bifunctional protein involved in biosynthesis of the lipopolysaccharide core precursor ADP-L-glycero-D-manno-heptose. J Bacteriol 182, 488-497. Weiser, J.N., Pan, N., McGowan, K.L., Musher, D., Martin, A., Richards, J., 1998, Phosphorylcholine on the lipopolysaccharide of Haemophilus influenzae contributes to persistence in the respiratory tract and sensitivity to serum killing mediated by C-reactive protein. J Exp Med 187, 631-640.
摘要: Pasteurella multocida (多殺性巴斯德桿菌)為能引起多種重要動物疾病的病原菌,其脂多醣體抗原 (lipopolysaccharide somatic antigen)是主要的毒力因子之一,傳統上可用瓊膠擴散沉澱反應 (AGP)將P. multocida分為16種不同體抗原血清型,但此方法需製備高免血清且操作時間需1-2天,另一方面,目前在文獻中只有少數幾種P. multocida之體抗原之成份與其生合成基因序列被發表。本研究以PCR技術,成功增幅18株P. multocida標準株之體抗原合成基因序列,定序結果發現,依其序列可將P. multocida之體抗原合成基因分為5種 (G1-G5)不同型別,其中G1為能引起家禽霍亂之體抗原1型之禽源P. multocida (A:1);G2為體抗原2型與5型之菌株;G3為體抗原3型及4型之禽源菌株;G4為能引起豬萎縮性鼻炎之體抗原3型菌株及其他體抗原血清型10、11、12、15和16菌株;G5則為分離自人類之體抗原13型菌株。本研究亦利用PCR增幅與限制酶切割圖譜多型性 (restriction fragment length polymorphism,RFLP)來快速區分P. multocida標準株之體抗原型別,此技術並可應用於田間禽源與豬源P. multocida體抗原型別之快速鑑定,本研究為第一篇利用PCR/RFLP來鑑定P. multocida體抗原型別之報導。
Pasteurella multocida is the causative agent of many diseases in a wide range of domestic animals. Its lipopolysaccharide (LPS) somatic antigen is one of key virulence factors. Conventionally, P. multocida can be divided into 16 serotypes based on LPS somatic antigens by using the agar gel diffusion precipitation test (AGP). However, this method needs preparation of serotype-specific antisera, and takes few days to complete the work. To date, only a few of LPS structures and genome sequences for biosynthesis of LPS in P. multocida have been determined. In this study, we used PCR to amplify the biosynthesis genes of lipopolysaccharide somatic antigen of 18 reference strains of P. multocida. Based on sequencing results of the amplified genes, the biosynthesis genes of LPS of P. multocida could be divided into 5 groups (G1-G5). Strains belong to G1 are serotype A:1 which cause fowl cholera. Stains belong to G2 are LPS serotype 2 and 5. Strains belong to G3 are LPS serotype 3 and 4 that cause fowl cholera. Strains belong to G4 are LPS serotype 3 which causes atrophic rhinitis in pigs and other LPS serotypes such as 10, 11, 12, 15, and 16. Strains belong to G5 are LPS serotype 13 that infects humans. This study also develops a PCR/RFLP method for rapid identification of the LPS serotypes of reference strains of P. multocida quickly. Moreover, this method can be used to identify types of somatic antigen of field strains of P. multocida. This is the first report on development of a PCR/PFLP method for rapid identification of the somatic antigen serotypes of P. multocida.
URI: http://hdl.handle.net/11455/66302
其他識別: U0005-1908201005402200
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-1908201005402200
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