Please use this identifier to cite or link to this item:
Expression of Capsid Proteins and Replication Proteins of Waterfowl Circoviruses in Baculovirus System and Application in Serological Survey
|引用:||王重雄、羅竹芳。利用昆蟲細胞及幼蟲生產外源蛋白。中華昆蟲特刊第五號。153-161。1990。 陳建宏。鵝環狀病毒封套蛋白與複製蛋白在大腸桿菌之表現及其在血清學診斷上之應用。國立中興大學碩士論文。2003。 王芃宣。台灣地區鵝環狀病毒與鴨環狀病毒之PCR檢測、基因型分析與宿主特異性研究。國立中興大學碩士論文。2005。 李洸鉑。以大腸桿菌表現水禽環狀病毒外殼蛋白與複製蛋白並應用於血清抗體之調查。國立中興大學碩士論文。2005。 林佳蓉。豬環狀病毒第二型外殼蛋白之表現及應用。國立中興大學碩士論文。2004。 陳秋麟、張伯俊、謝快樂、李敏旭、歐繕嘉、陳建宏、沈瑞鴻。應用聚合酶連鎖反應檢測水禽中之鵝環狀病毒。台灣獸醫誌 30:82-88, 2004。 Acharya A, Gopinathan KP. Indentification of an enhancer-like element in the polyhedron gene upstream region of Bombyx mori nucleopolyhedrovirus. J Gen Virology 82: 2811-19, 2001. Adair BM. Immunopathogenesis of chicken anemia virus infection. Developmental and comparative immunology 24:247-255, 2000. Allan GM, McNeilly F, and Meehan BM. A sequential study of experimental infection of pigs with porcine circovirus and porcine parvovirus: Immunostaining of cryostat sections and virus isolation. Journal of veterinary medicine B Infectious diseases and veterinary public health 47:81-94, 2000. Allan GM, McNeilly F, Meehan BM, Kennedy S, Mackie DP, Ellis JA, Clark EG, Espuna E, Saubi N, Riera P, Botner A, and Charreyre C. Isolation and characterization of circoviruses from pigs with wasting syndromes in Spin, Denmark and Northern Ireland. Veterinary Microbiology 66:115-123, 1999. Allan GM, Meehan BM, Todd D, Kennedy S, McNeilly F, Ellis JA, Clark EG, Harding J, Espuna E, Botner A, and Charreyre C. Novel porcine circoviruses from pig with wasting disease syndromes. The Veterinary record 142:467-468, 1998. Allan GM, Phenix KV, Todd D, and McNulty MS. Some biological and physico-chemical properties of porcine circovirus. Zentralbl Veterinarmed B 41:17-26, 1994. Ball NW, Smyth JA, Weston JH, Borghmans BJ, Palya V, Glavits R, Ivanics E, Dan A, and Todd D. Diagnosis of goose circovirus infection in Hungarian geese samples using polymerase chain reaction and dot blot hybridization tests. Avian pathology 33: 51-58, 2004. Biagini P, Todd D, Bendinelli M, and 8 other authors. Anellovirus. In Virus Taxonomy. Eighth Report 455 of the International Committee on Taxonomy of Viruses, Edited by Fauquet CM, Mayo MA, Maniloff J, Desselberger U, and Ball LA. London: Elseviver/Academic Press pp.335–341, 2004. Bissard GW, Rohrmann GF. Baculovirus diversity and molecular biology. Ann. Rev. Entomol. 35: 127-55, 1990. Bonning BC, Hammock BD. Development of recombinant baculovirus for insect control. Annu. Rev. Entomol. 41: 191-210, 1996. Burgess S. Molecular weights of Lepidopteran baculovirus DNAs: derivation by electron microscopy. J Gen Virol 37: 501-10, 1977. Chang HC, Lin TL, and Wu CC. DNA-mediated vaccination against infectious bursal disease in chickens. Vaccine 20:328-335, 2001. Chen CL, Chang PC, Lee MS, Shien JH, Ou SJ, and Shieh HK. Development of a polymerase chain reaction procedure for detection and differentiation of duck and goose circovirus. Avian disease 50:92-95, 2006. Chen CL, Chang PC, Lee MS, Shien JH, Ou SJ, and Shieh HK. Nucleotide sequences of goose circovirus isolated in Taiwan. Avian pathology 32:165-171, 2003. Cheung AK, and Bolin SR. Kinetics of porcine circovirus type 2 replication. Archives of Virology 147:43-58, 2002. Cheung AK. Identification of the essential and non-essential transcription units for protein synthesis, DNA replication and infectious virus production of porcine circovirus type1. Archives of Virology 149:975-988, 2004. Cheung AK. Transcriptional analysis of porcine circovirus type 2. Virology 305:168-180, 2003. Choi C, and Chae C. In-situ hybridization for the detection of porcine circovirus in pigs with postweaning multisystemic wasting syndrome. Journal of comparative pathology 121:265-270, 1999. Choi KS, Nah JJ, Choi CU, Ko YJ, Sohn HJ, Libeau G, Kang SY, Joo YS. Monoclonal antibody-based competitive ELISA for simultaneous detection of rinderpest virus and peste des petits ruminants virus antibodies. Vet Microbiol. 96:2 1-16, 2003. Ciccarone VC, Polayes D, Luckow VA. Generation of Recombinant Baculovirus DNA in E. coli Using Baculovirus Shuttle Vector. Volume 13, U. Reischt, ed. (Totowa, NJ: Humana Press Inc.), 1997. Claessens JA, Schrier CC, Mockett AP, Jagt EH, and Sondermeijer PJ. Molecular cloning and sequence analysis of the genome of chicken anaemia agent. The Journal of general virology 72:2003-2006, 1991. Clark EG. Post-weaning multisystemic wasting syndrome. Proc Am Assoc Swine Pract 28:499-501, 1997. Crowther RA, Berriman JA, Curran WL, Allan GM, and Todd D. Comparison of the structures of three circoviruses: chicken anemia virus, porcine circovirus type2, and beak feather disease virus. Journal of Virology 77:13036-13041, 2003. De Bore GF, van Roozelaar DJ, Moormann RJ, Jeurissen SH, van den Wijngaard JC, Hilbink F, and Koch G. Interaction between chicken anaemia virus and live Newcastle disease vaccine. Avian pathology 23:263-275, 1994. Dean FB, Nelson JR, Giesler TL, and Lasken RS. Rapid amplification of plasmid and phage DNA using Phi29 DNA polymerase and multiply-primed rolling circle amplification. Genome research 11:1095-1099, 2001. Eills J, Hassard L, Clark E, Harding J, Allan G, Wilson P, Strokappe J, Martin K, McNeilly F, Meehan B, Todd D, and Haines D. Isolation of circovirus from lesions of pigs with postweaning multisystemic wasting syndrome. The Canadian veterinary journal 39:44-51, 1998. Eisenberg SW, van Asten AJ, van Ederen AM, and Dorrestein GM. Detection of circovirus with a polymerase chain reaction in the ostrich (Struthio camelus) on a farm in The Netherlands. Veterinary microbiology 95:27-38, 2003. Engelhard EK, Kam M, L.N.W, Washburm JO, Volkman LE. The insect tracheal system: A conduit for the systemic spread of Autographa californica Mutiple nuclear polyhedrosis virus. Proc. Natl. Acad. Sci. USA. 91: 3224-27, 1994. Gelderblom H, Kling S, Lurz R, Tischer I, and von Bulow V. Morphological characterization of chicken anaemia agent (CAA). Archives of virology 109:115-120, 1989. Ghosh, S, Khalid PM, Banerjee K, Sarin SK, Hasnain SE. Baculovirus as Mammalian Cell Expression Vector for Gene Therapy: An Emerging Strategy Mol Ther. 6 (1): 5-11, 2002. Goldsmith TL. Documentation of passerine circoviral infection. Proc Assoc Avian Vet 16:349, 1995. Gough RE and Drury SE. Circovirus-like particles in the bursae of young racing pigeons. The Veterinary record 138:167, 1996. Grula MA, Buller PL, Weaver RF. Alpha-amanitin resistant viral RNA synthesis in nuclei isolated from nuclear polyhedrosis virus-infected Heliothis zea larvae and Spodoptera frugiperda cells. Virology 38: 916–921, 1981. Gut-Winiarska M, Jacobs L, Kerstens H, Bienkowska-Szewczyk K. A highly specific and sensitive sandwich blocking ELISA based on baculovirus expressed pseudorabies virus glycoprotein B. J Virol Methods. 88(1): 63-71, 2000. Hamel AL, Lin LL, and Nayar GP. Nucleotide sequence of porcine circovirus associated with postweaning multisystemic wasting syndrome in pigs. Journal of virology 72: 5262-5267, 1998. Harrap KA. The structure of nuclear polyhedrosis viruses.I. The inclusion body. Virology 50: 114-23, 1972a. Harrap KA. The structure of nuclear polyhedrosis viruses. II. The virus particle. Virology 50: 124-32, 1972b. Harding JC. Postweaning multisystemic wasting syndrome (PMWS): preliminary epidemiology and clinical presentation. Pro Am Assoc Sw Prac 28th Ann Meet. Quebec Canada 28:503, 1997. Hattermann K, Schmitt C, Soike D, and Mankertz A. Cloning and sequencing of Duck circovirus (DuCV). Archives of virology 148:2471-2480, 2003. Heath L, Martin DP, Warburton L, Perrin M, Horsfield W, Kingsley C, Rybicki EP, and Williamson AL. Evidence of unique genotypes of beak and feather disease virus in southern Africa. Journal of virology 78:9277-9284, 2004. Ignoffo CM. Entomopathogens as insecticides. Environ Lett. 8(1): 23-40, 1975. Islam MR, Johne R, Raue R, Todd D, and Muller H. Sequence analysis of the full-length cloned DNA of a chicken anaemia virus (CAV) strain from Bangladesh: evidence for genetic grouping of CAV strains based on the deduced VP1 amino acid sequences. Journal of veterinary medicine 49:332-337, 2002. Johne R, Fernandez-de-Luco D, Hofle U, and Muller H. Genome of a novel circovirus of starlings, amplified by multiply primed rolling-circle amplification. The Journal of general virology 87:1189-1195, 2006. Kitts PA, Ayres MD, Possee RD. Linearization of baculovirus DNA enhances the recovery of recombinant virus expression vectors. Nucleic Acids Res 18: 5667-72, 1990. Kitts PA. Possee RD. A method for producing recombinant baculovirus expression vectors at high frequency. Biotechniques 14: 810-817, 1993. Kiupel M, Stevenson GW, Mittal SK, Clark EG, and Haines DM. Circovirus-like viral associated disease in weaned pigs in Indiana. Veterinary pathology 5:303-307, 1998. Koch G, van Roozelaar DJ, Verschueren CA, van der Eb AJ, and Noteborn MH. Immunogenic and protective properties of chicken anaemia virus proteins expressed by baculovirus. Vaccine 13:763-770, 1995. Latimer KS, Rakich PM, Niagro FD, Ritchie BW, Steffens WL, Campagnoli RP, Pesti DA, and Lukert PD. An updated review of psittacine beak and feather disease. J Assoc avian Vet 5:211-220, 1991. Leisy DJ, Rohrmann GF. Characterization of the replication of plasmids containing hr sequences in baculovirus-infected Spodoptera frugiperda cells. Virology 196: 722–730, 1993. Liu Q, Tikoo KS, and Babiuk LA. Nuclear localization of the ORF2 protein encoded by porcine circovirus type2. Virology 285:91-99, 2001. Lukert PD, de Boer GF, Dale JL, Keese P, McNulty MS, Randles JW, and Tischer I. The Circoviridae In Virus Taxonomy Classification and Nomenclature of Viruses Sixth Report of the International Committee of Taxonomy of Viruses. pp 166-168, 1995. Luo L, Li Y, Cannon PM, Kim S, Kang CY. Chimeric gag-V3 virus-like particles of human immunodeficiency virus induce virus-neutralizing antibodies. Proc Natl Acad Sci U S A. 89(21):10527-10531, 1992. Luque T. O’Reilly DR. Generation of baculovirus expression vectors. Mol. Biotechnol 13: 153-63, 1999. Majima K, Kobara R, Maeda S. Divergence and evolution of homologous regions of Bombyx mori nuclear polyhedrosis virus. J Virol 67: 7513-21, 1993. Mankertz A, and Hillenbrand B. Replication of porcine circovirus type1 requires two proteins encoded by the viral rep gene. Virology 279:429-438, 2001. Mankertz A, Domingo M, Folch JM, LeCann P, Jestin A, Segales J, Chmielewicz B, Plana-Duran J, and Soike D. Characterisation of PCV-2 isolates from Spain, Germany and France. Virus research 66:65-77, 2000a. Mankertz A, Hattermann K, Ehlers B, and Soike D. Cloning and sequencing of columbid circovirus (CoCV), a new circovirus from pigeons. Archives of virology 145:2469-2479, 2000b. Mankertz A, Mueller B, Steinfeldt T, Schmitt C, and Finsterbusch T. New reporter gene-based replication assay reveals exchangeability of replication factors of Porcine Circovirus type1 and type2. The Journal of general virology 77:9885-9893, 2003. Mankertz A, Persson F, Mankertz J, Blaess G, and Buhk HJ. Mapping and characterization of the origin of DNA replication of porcine circovirus. The Journal of general virology 71: 2562-2566, 1997. Marchal ID, Jarvis L, Cacan R, and Verbert A. Glycoproteins from insect cells: sialylated or not? Biol Chem 382: 151-159, 2001. McFerran JB, McNulty MS, Curran WL. Diagnosis of avian viral diseases by electron microscopy. American Journal of veterinary research 39:505-508, 1978. McKeown NE, Opriessnig T, Thomas P, Guenette DK, Elvinger F, Fenaux M, Halbur PG, and Meng XJ. Effects of porcine circovirus type 2 (PCV2) maternal antibodies on experimental infection of piglets with PCV2. Clinical and diagnostic laboratory immunology 12:1347-1351, 2005. McNulty MS. Chicken anaemia agent: a review. Avian pathology 20:187-203, 1991. Meehan BM, Creelan JL, McNulty MS, and Todd D. Sequence of porcine circovirus DNA: affinities with plant circoviruses. The Journal of general virology 78:221-227, 1997. Miller LK. Baculovirus as gene expression vectors. Annu. Rev Microbiol 42: 177-199, 1988. Min W, Lillehoj HS, Burnside J, Weining KC, Staeheli P, and Zhu JJ. Adjuvant effects of IL-1beta, IL-2, IL-8, IL-15, IFN-alpha, IFN-gamma TGF-beta4 and lymphotactin on DNA vaccination against Eimeria acervulina. Vaccine 20:267-274, 2001. Morozov I, Sirinarumitr T, Sorden SD, Halbur PG, Morgan MK, Yoon KJ, and Paul PS. Detection of a novel strain of porcine circovirus in pigs with postweaning multisystemic wasting syndrome. Journal of clinical microbiology 36:2535-2541, 1998. Mwwhan BM, McNeilly F, Todd D, Kennedy S, Jewhurst VA, Ellis JA, Hassard LE, Clark EG, Haines DM, and Allan GM. Characterization of novel circovirus DNAs associated with wasting syndromes in pigs. The Journal of general virology 79:2171-2179, 1998. Niagro FD, Forsthoefel AN, Lawther RP, Kamalanathan L, Ritchie BW, Latimer KS, and Lukert PD. Beak and feather disease virus and porcine circovirus genomes: intermediates between the geminiviruses and plant circoviruses. Archives of virology 143: 1723-1744, 1998. Niel C, Diniz-Mendes L, and Devalle S. Rolling-circle amplification of Torque teno virus (TTV) complete genomes from human and swine sera and identification of a novel swine TTV genogrup. The Journal of general virology 86:1343-1347, 2005. Nishizawa T, Okamoto H, Nishizawa T, and Ukita M. A novel DNA virus (TTV) associated with elevated transaminase levels in posttransfusion hepatitis of unknown etiology. Biochemical and biophysical research communications 1241:92-97, 1997. Noteborn MH, de Boer GF, van Roozelaar DJ, Karreman C, Kranenburg O, Vos JG, Jeurissen SH, Hoeben RC, Zantema A, Koch G, and et al. Characterization of cloned chicken anemia virus DNA that contains all elements for the infectious replication cycle. The Journal of general virology 65:3131-3139, 1991. Noteborn MH, Verschueren CA, van Ormondt H, and van der Eb AJ. Chicken anemia virus strains with a mutated enhancer/promoter region share reduced virus spread and cytopathogenicity. Gene 223:165-172, 1998. Okamoto H, Nishizawa T, Kato N, Ukita M, Ikeda H, Iizuka H, Miyakawa Y, and Mayumi M. Molecular cloning and characterization hepatitis of unknown etiology. Hepatology research 10:1-16, 1998. O''Reilly DR, Hails RS, Kelly TJ. The impact of host developmental status on baculovirus replication. J Invertebr Pathol Nov 72(3): 269-275, 1998. Pages-Mante A, Saubi N, Artigas C, and Espuna E. Exprimental evaluation of an inactivated vaccine against chicken anaemia virus. Avian Pathol 26:721-729, 1997. Pass DA and Perry RA. The pathology of psittacine beak and feather disease. Australian veterinary journal 61:69-74, 1984. Phenix KV, Weston JH, Ypelaar I, Lavazza A, Smyth JA, Todd D, Wilcox GE, and Raidal SR. Nucleotide sequence analysis of a novel circovirus of canaries and its relationship to other members of the genus Circovirus of the family Circoviridae. The Journal of general virology 82:2805-2809, 2001. Racine S, Khevar A, Gagnon CA, Charbonneau B, and Dea S. Eucaryotic expression of the nucleocapsid protein gene of porcine circovirus type 2 and use of the protein in an indirect immunofluorescence assay for serological diagnosis of postweaning multisystemic wasting syndrome in pigs. Clinical and diagnostic laboratory immunology 11:736-741, 2004. Raidal SR and Cross GM. The haemagglutination spectum of psittacine beak and feather disease virus. Avian pathology 23:621-630, 1994. Raidal SR, and Riddoch PA. A feather disease in Senegal doves (Strptopelia senegalensis) morphologically similar to psittacine beak and feather disease. Avian pathology 26:829-836, 1997. Rector A, Tachezy R, Van Doorslaer K, MacNamara T, Burk RD, Sundberg JP, and Van Ranst M. Isolation and cloning of papillomavirus from a North American porcupine by using multiply-primed rolling circle amplification: the Erethizon dorsatum papillomavirus type 1. Virology 331:449-456, 2005. Ritchie BW, Niagro FD, Lukert PD, Steffens WL, 3rd, and Latimer KS. Characterization of a new virus from cockatoos with psittacine beak and feather disease. Virology 171:83-88, 1989. Rosell C, Segales J, Plana-Duran T, Balasch M, Rodriguez-Arrioguez-Arrioja GM, Kennedy S,Allan GM, McNeilly F, Latimer KS, and Domingo M. Pathological, immunohistochemical and in situ hybridization studies of natural cases of post weaning multisystemic wasting syndrome (PMWS) in pigs. Journal of comparative pathology 120:59-78, 1999. Sakaguchi M, Nakamura H, Sonoda K, Hamada F, and Hirai K. Protection of chickens from Newcastle disease by vaccination with a linear plasmid DNA expressing the F protein of Newcastle disease virus. Vaccine 14:747-752, 1996. Scott AN, Beckett A, Smyth JA, Ball NW, Palya V, Todd D. Serological diagnosis of goose circovirus infections. Avian pathology 35(6):495-499, 2006. Shivaprasad HL, Hill D, Todd D, and Smyth JA. Circovirus infection in a Gouldian finch (Chloebia gouldiae). Avian pathology 33:525-529, 2004. Smith GE, Summers MD, Fraser MJ. Production of human beta interferon in insect cells infected with a baculovirus expresssion vector. Mol Cell Biol 3: 2156-2165, 1983. Smyth J, Soike D, Moffett D, Weston JH, and Todd D. Circovirus-infected geese studied by in situ hybridization. Avian pathology 34:227-232, 2005. Smyth J, Weston J, Moffett DA, and Todd D. Detection of circovirus infection in pigeons by in situ hybridization using cloned DNA probes. Journal of veterinary diagnostic investigation 13:475-482, 2001 . Smyth JA and Carroll BP. Circovirus infection in European racing pigeons. The Veterinary record 136:173-174, 1995. Soike D, Albrecht K, Hattermann K, Schmitt C, and Mankertz A. Novel circovirus in mulard ducks with developmental and feathering disorders. The Veterinary record 154:792-793, 2004. Soike D, Kohler B and Albrecht K. A circovirus-like infection of geese related to a runting syndrome. Avian pathology 28:199-202, 1999. Soike D. Circovirus infections in pigeons. Tierärztliche Praxis 25:52-54, 1997. Soike D. Emerging virus – a challenge to diagnostic Electron Microscopy. Microscopy and microanalysis 9:522, 2003. Steenhuisen W, Jagt HJM, and Schrier CC. The use of a live attenuated CAV vaccine in breeder flocks in the Netherlands. Proceeding of the International Symposium on Infectious Bursal Disease and Infectious Chicken Anaemia, Rauischholzhausen, Germany: University of Giesen. pp. 482-497, 1994. Stevenson GW, Kiupek M, Mittal SK, and Kanitz CL. Ultrastructure of porcine circovirus in persistently infected PK-15 cells. Veterinary pathology 36:368-378, 1999. Stewart ME, Perry R, and Radidal SR. Identification of a novel circovirus in Australian ravens (Corvus coronoides) with feather disease. Avian pathology 35(2):86-92, 2006. Suarez DL, and Schultz-Cherry S. The effect of eukaryotic expression vectors and adjuvants on DNA vaccines in chickens using an avian influenza model. Avian diseases 44:861-868, 2000. Summers M, Anderson D. Granulosis virus deoxyribonucleic acid: a closed double-stranded molecular. J Virol 9: 710-713, 1972. Takahashi K., Iwasa Y., Hijikata M., and Mishiro S. Identification of a new human DNA virus (TTV-like mini virus, TLMV) intermediately related to TT virus and chicken anemia virus. Archives of virology 145:979-993, 2000. Thiem SM, Miller LK. Differential gene expression mediated by late,very late and hybrid baculovirus promoters. Gene 91: 87-94, 1990. Tischer I., Peters D., Rasch R., and Pociuli S. Replication of porcine circovirus: indu ction by glucosamine and cell cycle dependence. Archives of virology 96:39-57. 1987. Tischer I., Rasch R., and Tochtermann G. Characterization of papovavirus-and picornavirus-like partickes in permanent pig kidney cell lines. Zentralblatt für Bakteriologie 226:153-167, 1974. Todd D, Duchatel JP, Weston JH, Ball NW, Borghmans BJ, Moffett DA, and Smyth JA. Evaluation of polymerase chain reaction and dot blot hybridisation tests in the diagnosis of pigeon circovirus infections. Veterinary microbiology 89:1-16, 2002. Todd D, McNulty MS, Adair BM, and Allan GM. Animal circoviruses. Advances in virus research 57:1-70, 2001a. Todd D, Niagro FD, Ritchie BW, Curran W, Allan GA, Lukert PD, Latimer KS, Steffens III WL, and McNulty MS. Comparison of three animal viruses with circular single-stranded DNA genomes. Archives of virology 117:129-135, 1991. Todd D, Weston JH, Soike D, and Smyth JA. Genome sequence determinations and analyses of novel circoviruses from goose and pigeon. Virology 286:354-362, 2001b. Vielitz E, and Voss M. Experiences with a commercial CAV-vaccine. Proceedings of the International Symposium on Infectious Bursal Disease and Infectious Chicken Anaemia, Germany. University of Giesen. pp. 465-481, 1994. Warnes A, Fooks AR, Stephenson JR. Production of measles nucleoprotein in different expression systems and its use as a diagnostic reagent. J Virol Methods. 49(3): 257-68, 1994. Woods LW, Latimer KS, Barr BC, Niagro FD, Campagnoli RP, Nordhausen RW, and Castro AE. Circovirus-like infection in a pigeon. Journal of veterinary diagnostic investigation 5:609-612, 1993. Woods LW, Latimer KS, Niagro FD, Riddell C, Crowley AM, Anderson ML, Daft BM, Moore JD, Campagnoli RP, and Nordhausen RW. A retrospective study of circovirus infection in pigeons: nine cases (1986-1993). Journal of veterinary diagnostic investigation 6:156-164, 1994. Yu-Chen HU. Baculovirus as a highly efficient expression vector in insect and mammalian cells. Acta Pharmacol Sin. 26(4): 405-416, 2005. Yuasa N, Taniguchi T, and Yoshida I. Isolation and some characteristics of an agent inducing anemia in chicks. Avian diseases 23:366-385, 1979.|
|摘要:||水禽環狀病毒為新興的水禽病原，可分為鵝環狀病毒與鴨環狀病毒，病毒感染後易造成免疫抑制，因而增加其它疾病的罹患率。先前本研究室曾使用E. coli表現系統，表現水禽類環狀病毒之複製蛋白(Rep)與外殼蛋白(Cap)，以作為診斷環狀病毒抗體之抗原。結果發現E. coli系統雖可順利表現病毒之Rep蛋白，但卻無法順利表現病毒之Cap蛋白。本研究之目的是以桿狀病毒系統，表現鴨鵝環狀病毒之Rep蛋白與Cap蛋白，並以此二表現蛋白為抗原，進行Western blot分析，以檢測台灣田間水禽環狀病毒之抗體。結果發現桿狀病毒系統可成功表現鴨鵝環狀病毒之全長Rep蛋白與Cap蛋白，進一步以此Cap及Rep蛋白為抗原，進行Western blot分析，以檢測鴨鵝環狀病毒之抗體，結果發現所檢測到之環狀病毒抗體力價皆不高，顯示水禽環狀病毒感染後可能無法產生高力價抗體。這是第一篇以桿狀病毒系統，成功表現出水禽環狀病毒之全長複製蛋白(Rep)與外殼蛋白(Cap)之報告。|
Waterfowl circoviruses are emerging diseases of waterfowls and these viruses could be divided into goose circovirus (GoCV) and duck circoviruses (DuCV). The infection of waterfowl circoviruses might lead to immuno suppression and increase the incidence of other diseases. This laboratory previously used the E. coli expression system to express the replication protein (Rep) and capsid protein (Cap) of GoCV and DuCV but found that the only the Rep protein but not the Cap protein could be expressed. The goal of this study is to use the baculovirus expression system to express the Rep and Cap proteins of GoCV and DuCV and to use these proteins as antigens to detect the antibodies against GoCV and DuCV in geese and ducks. The result shows that Rep and Cap proteins of GoCV and DuCV could all be successfully expressed in baculovirus expression system. Moreover, Western blot assays using these proteins as the antigens showed that the antibody titers against GoCV and DuCV were low in sera collected from geese and ducks in the field. This result suggests that the infection of GoCV and DuCV might produce only a low level of antibody response. This is the first report on the expression of the full-length Rep and Cap proteins of GoCV and DuCV.
|Appears in Collections:||微生物暨公共衛生學研究所|
Show full item record
TAIR Related Article
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.