Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/66433
標題: 鴨環狀病毒感染性選殖株之構築與核酸疫苗 之研發
Construction of infectious clones and development of DNA vaccines for duck circovirus
作者: 陳巧津
Chen, Ciao-Jin
關鍵字: duck circovirus
鴨環狀病毒
infectious clones
DNA vaccine
感染性選殖株
核酸疫苗
出版社: 獸醫微生物學研究所
引用: 王芃宣。台灣地區鵝環狀病毒與鴨環狀病毒之PCR檢測、基因型分析與宿主特異性研究。國立中興大學碩士論文。2005。 陳秋麟、張伯俊、謝快樂、李敏旭、歐繕嘉、陳建宏、沈瑞鴻。應用聚合酶連鎖反應檢測水禽中之鵝環狀病毒。台灣獸醫誌 30:82-88, 2004。 Adair BM. Immunopathogenesis of chicken anemia virus infection. Developmental and Comparative Immunology 24:247-255, 2000. Allan GM, and Ellis JA. Porcine circoviruses: a review. Journal of Veterinary Diagnostic Investigation 12:3-14, 2000. Biagini P, Todd D, Bendinelli M et al. Anellovirus. In Virus Taxonomy. Eighth Report 455 of the International Committee on Taxonomy of Viruses, Edited by Fauquet CM, Mayo MA, Maniloff J, Desselberger U, and Ball LA. London: Elseviver/Academic Press pp.335–341, 2004. Boyer JC and Haenni AL. Infectious transcripts and cDNA clones of RNA viruses. Virology 198:415–426, 1994. Chang HC, Lin TL, and Wu CC. DNA-mediated vaccination against infectious bursal disease in chickens. Vaccine 20:328-335, 2001. Chen CL, Chang PC, Lee MS, Shien JH, Ou SJ, and Shieh HK. Development of a polymerase chain reaction procedure for detection and differentiation of duck and goose circovirus. Avian Disease 50:92-95, 2006. Colter JS, Bird HH, Moyer AW, and Brown RA. Infectivity of ribonucleic acid isolated from virus-infected tissues. Virology 4:522-532, 1957. Danen-Van Oorschot AA, Fischer DF, Grimbergen JM, Klein B, Zhuang S, Falkenburg JH. Proceedings of the National Academy of Sciences of the United States of America 94: 5843–5847, 1997. De Bore GF, van Roozelaar DJ, Moormann RJ, Jeurissen SH, van den Wijngaard JC, Hilbink F, and Koch G. Interaction between chicken anaemia virus and live Newcastle disease vaccine. Avian Pathology 23:263-275, 1994. Dean FB, Nelson JR, Giesler TL, and Lasken RS. Rapid amplification of plasmid and phage DNA using Phi29 DNA polymerase and multiply-primed rolling circle amplification. Genome Research 11:1095-1099, 2001. Diderholm, H., Stenkvist B, Ponten J, and Wesslen T. Transformation of bovine cells in vitro after inoculationof Simian Virus 40 or its nucleic acid. Experimental cell research 37: 452-459, 1965. DiMayorca, G. A., Eddy B, Stewart SE, Hunter WS, Friend C, and Bendich A. Isolation of infectious deoxyribonucleic acid from SE polyomainfected tissue cultures. Proceedings of the National Academy of Sciences of the United States of America 45:1805-1807,1959. Fringuelli E, Scott ANJ, Beckett A, McKillen J, Smyth JA, Palya V, Glavits R, Ivanics E, Mankertz A, Franciosini1 MP and Todd D. Diagnosis of duck circovirus infections by conventional and real-time polymerase chain reaction tests. Avian Pathology 34:495-500, 2005. Eisenberg SW, van Asten AJ, van Ederen AM, and Dorrestein GM. Detection of circovirus with a polymerase chain reaction in the ostrich (Struthio camelus) on a farm in The Netherlands. Veterinary Microbiology 95:27-38, 2003. Gelderblom H, Kling S, Lurz R, Tischer I, and von Bulow V. Morphological characterization of chicken anaemia agent (CAA). Archives of Virology 109:115-120, 1989. Gerber, P. An infectious deoxyribonucleic acid derived from vacuolating virus (SV40). Virology 16:96-98,1962. Gierer A., and Schramm G, Naturforsch Z. Infectivity of ribonucleic acid from tobacco mosaic virus. Nature 177:702-703, 1956. Goldsmith TL. Documentation of passerine circoviral infection. Proc Assoc Avian Vet 16:349, 1995. Gorski F. DNA replication: the rolling circle model; critical remarks. Bulletin de l''Académie polonaise des sciences. Série des sciences biologiques 23:785-792, 1975. Gough RE and Drury SE. Circovirus-like particles in the bursae of young racing pigeons. The Veterinary record 138:167, 1996. Hamel AL, Lin LL, and Nayar GP. Nucleotide sequence of porcine circovirus associated with postweaning multisystemic wasting syndrome in pigs. Journal of Virology 72:5262-5267, 1998. Hanahan D, Lane D, Lipsich L, Wigler M and Botchan M. Characteristics of an SV40-plasmid recombinant and its movement into and out of the genome of a murine cell. Cell 21:127-139, 1980. Hattermann K, Schmitt C, Soike D, and Mankertz A. Cloning and sequencing of Duck circovirus (DuCV). Archives of Virology 148:2471-2480, 2003. Huppert J, and Sanders FK. An infective ribonucleic acid component from tumour cells infected with encephalomyocarditis virus. Nature 182:515-517, 1958. Islam MR, Johne R, Raue R, Todd D, and Muller H. Sequence analysis of the full-length cloned DNA of a chicken anaemia virus (CAV) strain from Bangladesh: evidence for genetic grouping of CAV strains based on the deduced VP1 amino acid sequences. Journal of Veterinary Medicine 49:332-337, 2002. Koch G, van Roozelaar DJ, Verschueren CA, van der Eb AJ, and Noteborn MH. Immunogenic and protective properties of chicken anaemia virus proteins expressed by baculovirus. Vaccine 13:763-770, 1995. Mankertz A, Domingo M, Folch JM, LeCann P, Jestin A, Segales J, Chmielewicz B, Plana-Duran J, and Soike D. Characterisation of PCV-2 isolates from Spain, Germany and France. Virus Research 66:65-77, 2000a. Mankertz A, Hattermann K, Ehlers B, and Soike D. Cloning and sequencing of columbid circovirus (CoCV), a new circovirus from pigeons. Archives of Virology 145:2469-2479, 2000b. Mark A. Israel, Hardy W. Chan, Sara L. Hourihan, Wallace P. Rowe, and Malcolm A. Martin. Biological activity of Polyoma virus viral DNA in mice and hamster. Journal of Virology 29:990-996, 1979. Mankertz A, Domingo M, Folch JM, LeCann P, Jestin A, Segales J, Chmielewicz B, Plana-Duran J, and Soike D. Characterisation of PCV-2 isolates from Spain, Germany and France. Virus Research 66:65-77, 2000a. Mankertz A, Persson F, Mankertz J, Blaess G, and Buhk HJ. Mapping and characterization of the origin of DNA replication of porcine circovirus. Journal of Virology 71:2562-2566, 1997. McFerran JB, McNulty MS, Curran WL. Diagnosis of avian viral diseases by electron microscopy. American Journal of Veterinary Research 39:505-508, 1978. Fenaux M, Halbur PG, Haqshenas G, Royer R, Thomas P, Nawagitgul P, Gill M, Toth TE, and Meng XJ. Cloned genomic DNA of type 2 porcine circovirus is infectious when injected directly into the liver and lymph nodes of pigs: characterization of clinical disease, virus distribution, and pathologic lesions. Journal of Virology 76:541–55, 2002. Fenaux M, Opriessnig T, Halbur PG, and Meng XJ. Immunogenicity and pathogenicity of chimeric infectious DNA clones of pathogenic porcine circovirus type 2 (PCV2) and nonpathogenic PCV1 in weanling pigs. Journal of Virology 77:11232–11243, 2003. Fenaux M, Opriessnig T, Halbu PG r, and Meng XJ. A Chimeric Porcine circovirus (PCV) with the immunogenic capsid gene of the pathogenic PCV type 2 (PCV2) cloned into the genomic backbone of the nonpathogenic PCV1 induces protective immunity against PCV2 infection in pigs. Journal of Virology 78:6297-6303, 2004. Min W, Lillehoj HS, Burnside J, Weining KC, Staeheli P, and Zhu JJ. Adjuvant effects of IL-1beta, IL-2, IL-8, IL-15, IFN-alpha, IFN-gamma TGF-beta4 and lymphotactin on DNA vaccination against Eimeria acervulina. Vaccine 20:267-274, 2001. Roca M, Balasc M h, Segale´s J, Calsamiglia M, Viaplana E, Urniza A, Hattermann K, Mankertz A, Plana-Dura´n J and Domingo M. In vitro and in vivo characterization of an infectious clone of a European strain of porcine circovirus type 2. Journal of General Virology 85:1259–1266, 2004. Niagro FD, Forsthoefel AN, Lawther RP, Kamalanathan L, Ritchie BW, Latimer KS, and Lukert PD. Beak and feather disease virus and porcine circovirus genomes: intermediates between the geminiviruses and plant circoviruses. Archives of Virology 143:1723-1744, 1998. Nishizawa T, Okamoto H, Nishizawa T, and Ukita M. A novel DNA virus (TTV) associated with elevated transaminase levels in posttransfusion hepatitis of unknown etiology. Biochemical and biophysical research communications 1241:92-97, 1997. Noteborn MH, Verschueren CA, van Ormondt H, and van der Eb AJ. Chicken anemia virus strains with a mutated enhancer/promoter region share reduced virus spread and cytopathogenicity. Gene 223:165-172, 1998. Ladekjaer-Mikkelsen AS, Nielsen J, Stadejek T, Storgaard T, Krakowka S, Ellis J, McNeilly F, Allan G, and Botner A. Reproduction of postweaning multisystemic wasting syndrome (PMWS) in immunostimulated and non-immunostimulated 3-week-old piglets experimentally infected with porcine circovirus type 2 (PCV2). Veterinary Microbiology 89:97-114, 2002. Pass DA and Perry RA. The pathology of psittacine beak and feather disease. Australian Veterinary Journal 61:69-74, 1984. Pages-Mante A, Saubi N, Artigas C, and Espuna E. Exprimental evaluation of an inactivated vaccine against chicken anaemia virus. Avian Pathology 26:721-729, 1997. Raidal SR, and Riddoch PA. A feather disease in Senegal doves (Strptopelia senegalensis) morphologically similar to psittacine beak and feather disease. Avian pathology 26:829-836, 1997. Ritchie BW, Niagro FD, Lukert PD, Steffens WL, 3rd, and Latimer KS. Characterization of a new virus from cockatoos with psittacine beak and feather disease. Virology 171:83-88, 1989. Girones R, Cote PJ, Hornbuckle W, Tennant BC, Gerin JL, Purcell RH, and miller RH. Complete nucleotide sequence of a molecular clone of woodchuck hepatitis virus that is infectious in the natural host. Proceedings of the National Academy of Sciences of the United States of America 86:1846-1849, 1989. Sakaguchi M, Nakamura H, Sonoda K, Hamada F, and Hirai K. Protection of chickens from Newcastle disease by vaccination with a linear plasmid DNA expressing the F protein of Newcastle disease virus. Vaccine 14:747-752, 1996. Shivaprasad HL, Hill D, Todd D, and Smyth JA. Circovirus infection in a Gouldian finch (Chloebia gouldiae). Avian pathology 33:525-529, 2004. Steenhuisen W, Jagt HJM, and Schrier CC. The use of a live attenuated CAV vaccine in breeder flocks in the Netherlands. Proceeding of the International Symposium on Infectious Bursal Disease and Infectious Chicken Anaemia, Rauischholzhausen, Germany: University of Giesen. pp. 482-497, 1994. Stewart ME, Perry R, and Radidal SR. Identification of a novel circovirus in Australian ravens (Corvus coronoides) with feather disease. Avian pathology 35(2):86-92, 2006. Soike D. Circovirus infections in pigeons. Tierärztliche Praxis 25:52-54, 1997. Soike D, Kohler B and Albrecht K. A circovirus-like infection of geese related to a runting syndrome. Avian Pathology 28:199-202, 1999. Soike D, Albrecht K, Hattermann K, Schmitt C, and Mankertz A. Novel circovirus in mulard ducks with developmental and feathering disorders. The Veterinary record 154:792-793, 2004. Suarez DL, and Schultz-Cherry S. The effect of eukaryotic expression vectors and adjuvants on DNA vaccines in chickens using an avian influenza model. Avian Diseases 44:861-868, 2000. . Smyth JA and Carroll BP. Circovirus infection in European racing pigeons. The Veterinary record 136:173-174, 1995. Tabin CJ, Bradley SM, Bargmann CI, Weinberg RA, Papageorge AG, Scolnick EM, Dhar R, Lowy DR. and Change EH. Nature 300, 143-149, 1982. Takahashi K., Iwasa Y., Hijikata M., and Mishiro S. Identification of a new human DNA virus (TTV-like mini virus, TLMV) intermediately related to TT virus and chicken anemia virus. Archives of Virology 145:979-993, 2000. Thomas W. Dubensky, Bruce A. Campbell, and Luis P. Villarrel. Direct transfection of viral and plamid DNA into the liver or spleen of mice. Proceedings of the National Academy of Sciences of the United States of America 81:7529-7533, 1984. Tischer I, Gelderblom H, Vettermann W, and Koch MA. A very small porcine virus with circular single-stranded DNA. Nature 295:64-66, 1982. Todd D, Niagro FD, Ritchie BW, Curran W, Allan GA, Lukert PD, Latimer KS, Steffens III WL, and McNulty MS. Comparison of three animal viruses with circular single-stranded DNA genomes. Archives of Virology 117:129-135, 1991. Vielitz E, and Voss M. Experiences with a commercial CAV-vaccine. Proceedings of the International Symposium on Infectious Bursal Disease and Infectious Chicken Anaemia, Germany. University of Giesen. pp. 465-481, 1994. Will H, Cattaneo R, Koch HG, Darai G and Schallern H. Nature 299:740-742,1982. Woods LW, Latimer KS, Barr BC, Niagro FD, Campagnoli RP, Nordhausen RW, and Castro AE. Circovirus-like infection in a pigeon. Journal of Veterinary Diagnostic Investigation 5:609-612, 1993. Woods LW, Latimer KS, Niagro FD, Riddell C, Crowley AM, Anderson ML, Daft BM, Moore JD, Campagnoli RP, and Nordhausen RW. A retrospective study of circovirus infection in pigeons: nine cases (1986-1993). Journal of Veterinary Diagnostic Investigation 6:156-164, 1994.
摘要: 鴨環狀病毒(Duck Circovirus,DuCV)為新興的水禽感染性病原體,主要侵犯鴨隻淋巴器官而引起免疫抑制,進而增加其它病原體感染機率。本研究有兩個目標,一為構築DuCV感染性選殖株(Infectious clone),並藉以研究DuCV基因表現之調控機制,二為研發DuCV核酸疫苗,以協助DuCV疫情之控制。在感染性選殖株之構築方面,本研究以PCR增幅DuCV之全長DNA,再進行接合反應,以形成DuCV之感染性DNA。構築方法是依據DuCV病毒株之序列所設計,經定點突變含有Eco RI切位,可與野生型區分。在動物實驗方面,每隻正番鴨採肌肉注射施打1.4 μg劑量之感染性DNA,攻毒後犧牲鴨隻取胸腺、華氏囊抽取病毒DNA並分析其序列,證實所構築之感染性DNA可成功感染鴨隻,並於其體內複製。DNA疫苗之製備則是將全長外殼蛋白(Capsid protein)基因選殖到載體pcDNA3.1/ TOPO,並以肌肉注射施打400 μg劑量,追加一次劑量後以DuCV野外株進行攻毒之後與對照組比較,定量PCR分析發現有施打DNA疫苗的鴨隻中,其華氏囊中之病毒量明顯較未施打疫苗的對照組少,顯示DNA疫苗亦有效。此外本研究也將全長之DuCV Capsid protein基因選殖到表現載體pET32a,再轉型到E. coli BL21 codon plus RIPL 中,大量表現重組蛋白,再以這些重組蛋白為抗原,利用western blot技術,偵測攻毒後不同週數鴨隻中血清抗體力價的變化。
Duck circovirus (DuCV) infection is an emerging disease of waterfowls. This disease invades the lymphoid organ of ducks and subsequently leads to immune suppression and increased incidence of other infections. The goal of this study is divided into two parts. The first is to construct infectious DNA of DuCV for investigating mechanisms involved in regulation of gene expression of DuCV, whereas the second is to develop DNA vaccine for the control of DuCV. Toward the first goal, we used PCR to amplify the full-length genome of DuCV. After ligation, the amplified DNA could serves as infectious DNA of DuCV. The infectious DNA of DuCV amplified by PCR contained an Eco RI cutting site and thus could be discriminated from wild-type viruses. For animal experiments, each Muscovy duck was injected intramuscularly with 1.4 μg of infectious DNA. After the injection, the DuCV DNA could be detected in the thymus and bursa of Fabricius of ducks. This result suggests that the infectious DNA could replicate in ducks. The DNA vaccine was constructed by cloning the gene encoding the capsid protein of DuCV into the vector pcDNA3.1/TOPO. Each Muscovy duck was injected with 400 μg of DNA and after a booster immunization, the ducks were challenged with a field strain of DuCV. The result of quantitative PCR tests showed that the virus titers in vaccinated ducks was lower than those in the control ducks. This result indicates that the DNA vaccine is effective. This study also clones the gene encoding the capsid protein of DuCV into the expression vector pET32a. After transformation into E. coli strain BL21 codon plus RIPL, the recombinant capsid protein was highly expressed. This recombinant was used as the antigen for Western blot analyses to determine the antibody tires against DuCV in ducks challenged with the virus.
URI: http://hdl.handle.net/11455/66433
其他識別: U0005-2008200801180100
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-2008200801180100
Appears in Collections:微生物暨公共衛生學研究所

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