Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/66470
標題: 大腸桿菌表現之VP243次單位疫苗對雞傳染性華氏囊病之免疫反應及保護效力之評估
Immune responses and protection efficacy of subunit vaccines containing E. coli expressed VP243 in chickens against infectious bursal disease
作者: 劉旭哲
Liu, Hsu-Che
關鍵字: 大腸桿菌
infectious bursal disease
雞傳染性華氏囊病
次單位疫苗
VP243
subunit vaccine
出版社: 微生物暨公共衛生學研究所
引用: 1. 呂榮修,1995,雞傳染華氏囊病。中華民國養雞協會,中華民國,65-74。 2. 李龍湖,2000,雞傳染性華氏囊病及其疫苗使用計畫。畜牧半月刊,64,22-24。 3. 陳政延,2011,反向遺傳技術伴隨野外強毒株感染加速傳染性華氏囊病毒之細胞馴化。 4. 蔡向榮,呂榮修,1993,台灣雞傳染性華氏囊病大流行之疫情分析。中華獸醫誌,19,249-258。 5. Arntzen, C.J., 1998, Pharmaceutical foodstuffs--oral immunization with transgenic plants. Nat Med 4, 502-503. 6. Azad, A.A., Barrett, S.A., Fahey, K.J., 1985, The characterization and molecular cloning of the double-stranded RNA genome of an Australian strain of infectious bursal disease virus. Virology 143, 35-44. 7. Azad, A.A., Jagadish, M.N., Brown, M.A., Hudson, P.J., 1987, Deletion mapping and expression in Escherichia coli of the large genomic segment of a birnavirus. Virology 161, 145-152. 8. Azad, A.A., McKern, N.M., Macreadie, I.G., Failla, P., Heine, H.G., Chapman, A., Ward, C.W., Fahey, K.J., 1991, Physicochemical and immunological characterization of recombinant host-protective antigen (VP2) of infectious bursal disease virus. Vaccine 9, 715-722. 9. Bayliss, C.D., Spies, U., Shaw, K., Peters, R.W., Papageorgiou, A., Muller, H., Boursnell, M.E., 1990, A comparison of the sequences of segment A of four infectious bursal disease virus strains and identification of a variable region in VP2. J Gen Virol 71 ( Pt 6), 1303-1312. 10. Becht, H., Muller, H., Muller, H.K., 1988, Comparative studies on structural and antigenic properties of two serotypes of infectious bursal disease virus. J Gen Virol 69 ( Pt 3), 631-640. 11. Ben Abdeljelil, N., Khabouchi, N., Mardassi, H., 2008, Efficient rescue of infectious bursal disease virus using a simplified RNA polymerase II-based reverse genetics strategy. Arch Virol 153, 1131-1137. 12. Berg, T.P., Gonze, M., Meulemans, G., 1991, Acute infectious bursal disease in poultry: Isolation and characterisation of a highly virulent strain. Avian Pathol 20, 133-143. 13. Beug, H., Muller, H., Grieser, S., Doederlein, G., Graf, T., 1981, Hematopoietic cells transformed in vitro by REVT avian reticuloendotheliosis virus express characteristics of very immature lymphoid cells. Virology 115, 295-309. 14. Bhanot, V., Balamurugan, V., Bhanuprakash, V., Venkatesan, G., Sen, A., Yadav, V., Yogisharadhya, R., Singh, R.K., 2009, Expression of P32 protein of goatpox virus in Pichia pastoris and its potential use as a diagnostic antigen in ELISA. J Virol Methods 162, 251-257. 15. Birghan, C., Mundt, E., Gorbalenya, A.E., 2000, A non-canonical lon proteinase lacking the ATPase domain employs the ser-Lys catalytic dyad to exercise broad control over the life cycle of a double-stranded RNA virus. EMBO J 19, 114-123. 16. Bloch, H., 1993, Edward Jenner (1749-1823). The history and effects of smallpox, inoculation, and vaccination. Am J Dis Child 147, 772-774. 17. Bottcher, B., Kiselev, N.A., Stel''Mashchuk, V.Y., Perevozchikova, N.A., Borisov, A.V., Crowther, R.A., 1997, Three-dimensional structure of infectious bursal disease virus determined by electron cryomicroscopy. J Virol 71, 325-330. 18. Brown, F., 1986, The classification and nomenclature of viruses: summary of results of the meeting so the International Committee on Taxonomy of Viruses in Sendai. Intervirology 25, 141-143. 19. Brown, M.D., Green, P., Skinner, M.A., 1994, VP2 sequences of recent European ''very virulent'' isolates of infectious bursal disease virus are closely related to each other but are distinct from those of ''classical'' strains. J Gen Virol 75 ( Pt 3), 675-680. 20. Caston, J.R., Martinez-Torrecuadrada, J.L., Maraver, A., Lombardo, E., Rodriguez, J.F., Casal, J.I., Carrascosa, J.L., 2001, C terminus of infectious bursal disease virus major capsid protein VP2 is involved in definition of the T number for capsid assembly. J Virol 75, 10815-10828. 21. Cavallaro, A.S., Mahony, D., Commins, M., Mahony, T.J., Mitter, N., 2011, Endotoxin-free purification for the isolation of bovine viral diarrhoea virus E2 protein from insoluble inclusion body aggregates. Microb Cell Fact 10, 57. 22. Chen, C.S., Suen, S.Y., Lai, S.Y., Chang, G.R., Lu, T.C., Lee, M.S., Wang, M.Y., 2005, Purification of capsid-like particles of infectious bursal disease virus (IBDV) VP2 expressed in E. coli with a metal-ion affinity membrane system. J Virol Methods 130, 51-58. 23. Chettle, N.J., Eddy, R.K., Wyeth, P.J., 1985, The isolation of infectious bursal disease virus from turkeys in England. Br Vet J 141, 141-145. 24. Chevalier, C., Lepault, J., Da Costa, B., Delmas, B., 2004, The last C-terminal residue of VP3, glutamic acid 257, controls capsid assembly of infectious bursal disease virus. J Virol 78, 3296-3303. 25. Chevalier, C., Lepault, J., Erk, I., Da Costa, B., Delmas, B., 2002, The maturation process of pVP2 requires assembly of infectious bursal disease virus capsids. J Virol 76, 2384-2392. 26. Cosgrove, A.S., 1962, An Apparently New Disease of Chickens: Avian Nephrosis. Avian Dis 6, 385-389. 27. Coulibaly, F., Chevalier, C., Gutsche, I., Pous, J., Navaza, J., Bressanelli, S., Delmas, B., Rey, F.A., 2005, The birnavirus crystal structure reveals structural relationships among icosahedral viruses. Cell 120, 761-772. 28. Crick, F.H., 1958, On protein synthesis. Symp Soc Exp Biol 12, 138-163. 29. Cummings, T.S., Broussard, C. T., Page, R. K., Thayer, S. G. & Lukert, P. D., 1986, nfectious bursal disease virus in turkeys. Veterinary Bulletin 56, 757-762. 30. Dietrich, J.B., Lorber, B., Kern, D., 1991, Expression of mammalian tyrosine aminotransferase in Saccharomyces cerevisiae and Escherichia coli. Purification to homogeneity and characterization of the enzyme overproduced in the bacteria. Eur J Biochem 201, 399-407. 31. Dobos, P., Hill, B.J., Hallett, R., Kells, D.T., Becht, H., Teninges, D., 1979, Biophysical and biochemical characterization of five animal viruses with bisegmented double-stranded RNA genomes. J Virol 32, 593-605. 32. Doong, S.R., Chen, Y.H., Lai, S.Y., Lee, C.C., Lin, Y.C., Wang, M.Y., 2007, Strong and heterogeneous adsorption of infectious bursal disease VP2 subviral particle with immobilized metal ions dependent on two surface histidine residues. Anal Chem 79, 7654-7661. 33. Edgar, S.A., 1966, Infectious bursal disease (Gumboro-disease) prevention and control. Annual Poultry Health and Management Short Course, Clemson, South Carolina 10, 93-98. 34. Edgar, S.A., Cho, Y., 1976, The epizootiology of infectious bursal disease and prevention of it by immunization. Dev Biol Stand 33, 349-356. 35. Ellis, R.W., 1999, New technologies for making vaccines. Vaccine 17, 1596-1604. 36. Fahey, K.J., Erny, K., Crooks, J., 1989, A conformational immunogen on VP-2 of infectious bursal disease virus that induces virus-neutralizing antibodies that passively protect chickens. J Gen Virol 70 ( Pt 6), 1473-1481. 37. Freund, J., Casalas-Ariet, J., Genghof, D. S., 1940, The Synergistic Effect of Paraffin-Oil Combined with Heat-Killed Tubercle-Bacilli. J. Immun. 38, 67-79. 38. Helenius, A., Aebi, M., 2001, Intracellular functions of N-linked glycans. Science 291, 2364-2369. 39. Hernandez, M., Villegas, P., Hernandez, D., Banda, A., Maya, L., Romero, V., Tomas, G., Perez, R., 2010, Sequence variability and evolution of the terminal overlapping VP5 gene of the infectious bursal disease virus. Virus Genes 41, 59-66. 40. Hitchner, S.B., 1963, Poultry Pathology Newsletter. 41. Hudson, P.J., McKern, N.M., Power, B.E., Azad, A.A., 1986, Genomic structure of the large RNA segment of infectious bursal disease virus. Nucleic Acids Res 14, 5001-5012. 42. Hunter, R.L., 2002, Overview of vaccine adjuvants: present and future. Vaccine 20 Suppl 3, S7-12. 43. Hurley, J.C., 1995, Endotoxemia: methods of detection and clinical correlates. Clin Microbiol Rev 8, 268-292. 44. Irigoyen, N., Garriga, D., Navarro, A., Verdaguer, N., Rodriguez, J.F., Caston, J.R., 2009, Autoproteolytic activity derived from the infectious bursal disease virus capsid protein. J Biol Chem 284, 8064-8072. 45. Islam, M.R., Zierenberg, K., Muller, H., 2001, The genome segment B encoding the RNA-dependent RNA polymerase protein VP1 of very virulent infectious bursal disease virus (IBDV) is phylogenetically distinct from that of all other IBDV strains. Arch Virol 146, 2481-2492. 46. Jackwood, D.J., Saif, Y.M., Moorhead, P.D., 1985, Immunogenicity and antigenicity of infectious bursal disease virus serotypes I and II in chickens. Avian Dis 29, 1184-1194. 47. Kibenge, F.S., Qian, B., Cleghorn, J.R., Martin, C.K., 1997, Infectious bursal disease virus polyprotein processing does not involve cellular proteases. Arch Virol 142, 2401-2419. 48. Kost, T.A., Condreay, J.P., 1999, Recombinant baculoviruses as expression vectors for insect and mammalian cells. Curr Opin Biotechnol 10, 428-433. 49. Koths, K., 1995, Recombinant proteins for medical use: the attractions and challenges. Curr Opin Biotechnol 6, 681-687. 50. Krieg, A.M., 2000, The role of CpG motifs in innate immunity. Curr Opin Immunol 12, 35-43. 51. Kristensen, K., Gyhrs, A., Lausen, B., Barington, T., Heilmann, C., 1996, Antibody response to Haemophilus influenzae type b capsular polysaccharide conjugated to tetanus toxoid in preterm infants. Pediatr Infect Dis J 15, 525-529. 52. Kuroda, S., Miyazaki, T., Otaka, S., Fujisawa, Y., 1993, Saccharomyces cerevisiae can release hepatitis B virus surface antigen (HBsAg) particles into the medium by its secretory apparatus. Appl Microbiol Biotechnol 40, 333-340. 53. Lagging, L.M., Meyer, K., Hoft, D., Houghton, M., Belshe, R.B., Ray, R., 1995, Immune responses to plasmid DNA encoding the hepatitis C virus core protein. J Virol 69, 5859-5863. 54. Lee, C.C., Ko, T.P., Chou, C.C., Yoshimura, M., Doong, S.R., Wang, M.Y., Wang, A.H., 2006a, Crystal structure of infectious bursal disease virus VP2 subviral particle at 2.6A resolution: implications in virion assembly and immunogenicity. J Struct Biol 155, 74-86. 55. Lee, M.S., Doong, S.R., Lai, S.Y., Ho, J.Y., Wang, M.Y., 2006b, Processing of infectious bursal disease virus (IBDV) polyprotein and self-assembly of IBDV-like particles in Hi-5 cells. Biotechnol Prog 22, 763-769. 56. Lejal, N., Da Costa, B., Huet, J.C., Delmas, B., 2000, Role of Ser-652 and Lys-692 in the protease activity of infectious bursal disease virus VP4 and identification of its substrate cleavage sites. J Gen Virol 81, 983-992. 57. Li, T.Y., Jin, N.Y., Wang, H.W., Guo, Z.R., Fang, H.H., An, R.G., Yin, Z., 2000, [Expression and characterization of HIV-1 Gag p17-p24 protein]. Sheng Wu Gong Cheng Xue Bao 16, 65-68. 58. Liu, W., Jiang, H., Zhou, J., Yang, X., Tang, Y., Fang, D., Jiang, L., 2010, Recombinant dengue virus-like particles from Pichia pastoris: efficient production and immunological properties. Virus Genes 40, 53-59. 59. Lombardo, E., Maraver, A., Caston, J.R., Rivera, J., Fernandez-Arias, A., Serrano, A., Carrascosa, J.L., Rodriguez, J.F., 1999, VP1, the putative RNA-dependent RNA polymerase of infectious bursal disease virus, forms complexes with the capsid protein VP3, leading to efficient encapsidation into virus-like particles. J Virol 73, 6973-6983. 60. Lombardo, E., Maraver, A., Espinosa, I., Fernandez-Arias, A., Rodriguez, J.F., 2000, VP5, the nonstructural polypeptide of infectious bursal disease virus, accumulates within the host plasma membrane and induces cell lysis. Virology 277, 345-357. 61. Lowrie, D.B., Silva, C.L., Colston, M.J., Ragno, S., Tascon, R.E., 1997, Protection against tuberculosis by a plasmid DNA vaccine. Vaccine 15, 834-838. 62. Lukert, P.D., Saif, Y.M., 2003, Infectious bursal disease. , Vol 11Lowa State University Press Ames. 63. Luque, D., Saugar, I., Rodriguez, J.F., Verdaguer, N., Garriga, D., Martin, C.S., Velazquez-Muriel, J.A., Trus, B.L., Carrascosa, J.L., Caston, J.R., 2007, Infectious bursal disease virus capsid assembly and maturation by structural rearrangements of a transient molecular switch. J Virol 81, 6869-6878. 64. Maraver, A., Clemente, R., Rodriguez, J.F., Lombardo, E., 2003a, Identification and molecular characterization of the RNA polymerase-binding motif of infectious bursal disease virus inner capsid protein VP3. J Virol 77, 2459-2468. 65. Maraver, A., Ona, A., Abaitua, F., Gonzalez, D., Clemente, R., Ruiz-Diaz, J.A., Caston, J.R., Pazos, F., Rodriguez, J.F., 2003b, The oligomerization domain of VP3, the scaffolding protein of infectious bursal disease virus, plays a critical role in capsid assembly. J Virol 77, 6438-6449. 66. Marcotte, N., Goosen, M.F.A., 1989, Delayed release of water-soluble macromolecules from polylactide pellets. Journal of Controlled Release 9, 75-85. 67. Martinez-Torrecuadrada, J.L., Caston, J.R., Castro, M., Carrascosa, J.L., Rodriguez, J.F., Casal, J.I., 2000, Different architectures in the assembly of infectious bursal disease virus capsid proteins expressed in insect cells. Virology 278, 322-331. 68. Martinez-Torrecuadrada, J.L., Saubi, N., Pages-Mante, A., Caston, J.R., Espuna, E., Casal, J.I., 2003, Structure-dependent efficacy of infectious bursal disease virus (IBDV) recombinant vaccines. Vaccine 21, 3342-3350. 69. McFerran, J.B., McNulty, M.S., McKillop, E.R., Connor, T.J., McCracken, R.M., Collins, D.S., Allan, G.M., 1980, Isolation and serological studies with infectious bursal disease viruses from fowl, turkeys and ducks: demonstration of a second serotype. Avian Pathol 9, 395-404. 70. McNulty, M.S., Allan, G.M., McFerran, J.B., 1979, Isolation of infectious bursal disease virus from turkeys. Avian Pathol 8, 205-212. 71. Miller, J.H., Ippen, K., Scaife, J.G., Beckwith, J.R., 1968, The promoter-operator region of the lac operon of Escherichia coli. J Mol Biol 38, 413-420. 72. Miyanohara, A., Imamura, T., Araki, M., Sugawara, K., Ohtomo, N., Matsubara, K., 1986, Expression of hepatitis B virus core antigen gene in Saccharomyces cerevisiae: synthesis of two polypeptides translated from different initiation codons. J Virol 59, 176-180. 73. Morton, C.L., Potter, P.M., 2000, Comparison of Escherichia coli, Saccharomyces cerevisiae, Pichia pastoris, Spodoptera frugiperda, and COS7 cells for recombinant gene expression. Application to a rabbit liver carboxylesterase. Mol Biotechnol 16, 193-202. 74. Muller, H., 1986, Replication of infectious bursal disease virus in lymphoid cells. Arch Virol 87, 191-203. 75. Muller, H., Becht, H., 1982, Biosynthesis of virus-specific proteins in cells infected with infectious bursal disease virus and their significance as structural elements for infectious virus and incomplete particles. J Virol 44, 384-392. 76. Muller, H., Lange, H., Becht, H., 1986, Formation, characterization and interfering capacity of a small plaque mutant and of incomplete virus particles of infectious bursal disease virus. Virus Res 4, 297-309. 77. Muller, H., Nitschke, R., 1987, The two segments of the infectious bursal disease virus genome are circularized by a 90,000-Da protein. Virology 159, 174-177. 78. Muller, H., Scholtissek, C., Becht, H., 1979, The genome of infectious bursal disease virus consists of two segments of double-stranded RNA. J Virol 31, 584-589. 79. Mundt, E., Beyer, J., Muller, H., 1995, Identification of a novel viral protein in infectious bursal disease virus-infected cells. J Gen Virol 76 ( Pt 2), 437-443. 80. Murray, R., Cohen, P., Hardegree, M.C., 1972, Mineral oil adjuvants: biological and chemical studies. Ann Allergy 30, 146-151. 81. Nick, H., Cursiefen, D., Becht, H., 1976, Structural and growth characteristics of infectious bursal disease virus. J Virol 18, 227-234. 82. Omar, A.R., Kim, C.L., Bejo, M.H., Ideris, A., 2006, Efficacy of VP2 protein expressed in E. coli for protection against highly virulent infectious bursal disease virus. J Vet Sci 7, 241-247. 83. Oppling, V., Muller, H., Becht, H., 1991, Heterogeneity of the antigenic site responsible for the induction of neutralizing antibodies in infectious bursal disease virus. Arch Virol 119, 211-223. 84. Patarroyo, M.E., Romero, P., Torres, M.L., Clavijo, P., Moreno, A., Martinez, A., Rodriguez, R., Guzman, F., Cabezas, E., 1987, Induction of protective immunity against experimental infection with malaria using synthetic peptides. Nature 328, 629-632. 85. Pedro-Viejo, A.B., 2008, [The international adoption waiting period: waiting experience and coping strategies]. Psicothema 20, 551-556. 86. Peters, M.A., Lin, T.L., Wu, C.C., 2004, Infectious bursal disease virus polyprotein expression arrests growth and mitogenic stimulation of B lymphocytes. Arch Virol 149, 2413-2426. 87. Pitcovski, J., Gutter, B., Gallili, G., Goldway, M., Perelman, B., Gross, G., Krispel, S., Barbakov, M., Michael, A., 2003, Development and large-scale use of recombinant VP2 vaccine for the prevention of infectious bursal disease of chickens. Vaccine 21, 4736-4743. 88. Possee, R.D., 1997, Baculoviruses as expression vectors. Current Opinion in Biotechnology 8, 569-572. 89. Raposo, G., Nijman, H.W., Stoorvogel, W., Liejendekker, R., Harding, C.V., Melief, C.J., Geuze, H.J., 1996, B lymphocytes secrete antigen-presenting vesicles. J Exp Med 183, 1161-1172. 90. Remond, M., Da Costa, B., Riffault, S., Parida, S., Breard, E., Lebreton, F., Zientara, S., Delmas, B., 2009, Infectious bursal disease subviral particles displaying the foot-and-mouth disease virus major antigenic site. Vaccine 27, 93-98. 91. Riedel, S., 2005, Edward Jenner and the history of smallpox and vaccination. Proc (Bayl Univ Med Cent) 18, 21-25. 92. Rong, J., Cheng, T., Liu, X., Jiang, T., Gu, H., Zou, G., 2005, Development of recombinant VP2 vaccine for the prevention of infectious bursal disease of chickens. Vaccine 23, 4844-4851. 93. Sanchez, A.B., Rodriguez, J.F., 1999, Proteolytic processing in infectious bursal disease virus: identification of the polyprotein cleavage sites by site-directed mutagenesis. Virology 262, 190-199. 94. Schnitzler, D., Bernstein, F., Muller, H., Becht, H., 1993, The genetic basis for the antigenicity of the VP2 protein of the infectious bursal disease virus. J Gen Virol 74 ( Pt 8), 1563-1571. 95. Shwed, P.S., Dobos, P., Cameron, L.A., Vakharia, V.N., Duncan, R., 2002, Birnavirus VP1 proteins form a distinct subgroup of RNA-dependent RNA polymerases lacking a GDD motif. Virology 296, 241-250. 96. Smith, G.E., Summers, M.D., Fraser, M.J., 1983, Production of human beta interferon in insect cells infected with a baculovirus expression vector. Mol Cell Biol 3, 2156-2165. 97. Snyder, D.B., 1990, Changes in the field status of infectious bursal disease virus. Avian Pathol 19, 419-423. 98. Snyder, D.B., Lana, D.P., Savage, P.K., Yancey, F.S., Mengel, S.A., Marquardt, W.W., 1988, Differentiation of infectious bursal disease viruses directly from infected tissues with neutralizing monoclonal antibodies: evidence of a major antigenic shift in recent field isolates. Avian Dis 32, 535-539. 99. Spies, U., Muller, H., Becht, H., 1987, Properties of RNA polymerase activity associated with infectious bursal disease virus and characterization of its reaction products. Virus Res 8, 127-140. 100. Tacken, M.G., Peeters, B.P., Thomas, A.A., Rottier, P.J., Boot, H.J., 2002, Infectious bursal disease virus capsid protein VP3 interacts both with VP1, the RNA-dependent RNA polymerase, and with viral double-stranded RNA. J Virol 76, 11301-11311. 101. Tacken, M.G., Van Den Beuken, P.A., Peeters, B.P., Thomas, A.A., Rottier, P.J., Boot, H.J., 2003, Homotypic interactions of the infectious bursal disease virus proteins VP3, pVP2, VP4, and VP5: mapping of the interacting domains. Virology 312, 306-319. 102. Tacket, C.O., Mason, H.S., Losonsky, G., Clements, J.D., Levine, M.M., Arntzen, C.J., 1998, Immunogenicity in humans of a recombinant bacterial antigen delivered in a transgenic potato. Nat Med 4, 607-609. 103. Temin, H.M., Baltimore, D., 1972, RNA-directed DNA synthesis and RNA tumor viruses. Adv Virus Res 17, 129-186. 104. Tokunaga, T., Yamamoto, H., Shimada, S., Abe, H., Fukuda, T., Fujisawa, Y., Furutani, Y., Yano, O., Kataoka, T., Sudo, T., et al., 1984, Antitumor activity of deoxyribonucleic acid fraction from Mycobacterium bovis BCG. I. Isolation, physicochemical characterization, and antitumor activity. J Natl Cancer Inst 72, 955-962. 105. Tokunaga, T., Yano, O., Kuramoto, E., Kimura, Y., Yamamoto, T., Kataoka, T., Yamamoto, S., 1992, Synthetic oligonucleotides with particular base sequences from the cDNA encoding proteins of Mycobacterium bovis BCG induce interferons and activate natural killer cells. Microbiol Immunol 36, 55-66. 106. van den Berg, T.P., Eterradossi, N., Toquin, D., Meulemans, G., 2000, Infectious bursal disease (Gumboro disease). Rev Sci Tech 19, 509-543. 107. Wolff, J.A., Williams, P., Acsadi, G., Jiao, S., Jani, A., Chong, W., 1991, Conditions affecting direct gene transfer into rodent muscle in vivo. Biotechniques 11, 474-485. 108. Xu, X.G., Tong, D.W., Wang, Z.S., Zhang, Q., Li, Z.C., Zhang, K., Li, W., Liu, H.J., 2011, Baculovirus virions displaying infectious bursal disease virus VP2 protein protect chickens against infectious bursal disease virus infection. Avian Dis 55, 223-229. 109. Yamaguchi, T., Ogawa, M., Miyoshi, M., Inoshima, Y., Fukushi, H., Hirai, K., 1997, Sequence and phylogenetic analyses of highly virulent infectious bursal disease virus. Arch Virol 142, 1441-1458. 110. Yao, K., Goodwin, M.A., Vakharia, V.N., 1998, Generation of a mutant infectious bursal disease virus that does not cause bursal lesions. J Virol 72, 2647-2654. 111. Yip, C.W., Yeung, Y.S., Ma, C.M., Lam, P.Y., Hon, C.C., Zeng, F., Leung, F.C., 2007, Demonstration of receptor binding properties of VP2 of very virulent strain infectious bursal disease virus on Vero cells. Virus Res 123, 50-56.
摘要: 雞傳染性華氏囊病 (Infectious Bursal Disease; IBD) 係由雞傳染性華氏囊病病毒 (Infectious Bursal Disease Virus; IBDV) 所引起之高度傳染性免疫抑制疾病。該疾病多好發於三至六週齡之年幼雞隻,目前台灣對於雞傳染性華氏囊病的控制方法普遍於母雞產蛋前利用接種不活化疫苗或活減毒疫誘導雛雞之移行抗體,再以疫苗接種來控制,然而,活減毒疫苗本身具有病毒毒力回復的問題且仍會造成華氏囊之輕微損傷。隨著科技的進步,次單位疫苗成為疫苗發展上一個重要的突破,不僅比起活減毒疫苗更安全,且在製備上更為容易。本次試驗之目的為評估以大腸桿菌表現之IBDV的VP243蛋白於雞隻體內對雞傳染性華氏囊病之免疫反應及保護效力。首先將構築好之重組質體pTri-VP243轉形至大腸桿菌Rosetta中進行蛋白表現,大腸桿菌經由IPTG誘導蛋白表現後可以得到可溶及不可溶之VP243蛋白,再以酵素連結免疫吸附試驗 (enzyme-linked immunosorbent assay; ELISA)及西方墨點法 (western blotting) 進行蛋白分析確認,並於穿透式電子顯微鏡 (transmission electron microscope; TEM) 下觀察到VP243形成之類病毒顆粒 (virus-like particle; VLP) 。本次試驗總共進行四次動物實驗,以無特定抗原 (specific pathogen free; SPF) 的雞隻進行免疫。分別測試未經純化之可溶或不可溶VP243蛋白、經蔗糖密度離心純化之VP243蛋白以及與不同佐劑混合後之VP243蛋白免疫雞隻後產生之免疫反應及保護效力。雞隻於第一週及第三週齡時進行蛋白免疫,於每週進行採血分離血清,並於第四週以口腔餵食進行4×102 EID50 / 200 μl之強毒株IBDV (very virulent IBDV) 的攻毒,攻毒後四天進行犧牲及解剖。分離之血清以酵素連結免疫吸附試驗 (ELISA) 進行抗IBDV之抗體力價測定,而解剖後採其華氏囊,並依據華氏囊損傷程度進行保護力之判斷。結果顯示,無論是可溶或不可溶之VP243蛋白皆可以於雞隻體內誘導顯著的抗體揚升;VP243之可溶蛋白經過蔗糖初步純化或混合其他佐劑進行免疫之組別,經過攻毒後四天的保護力皆不甚理想,而雞隻免疫以佛氏佐劑混合之VP243可溶蛋白的組別有較佳之保護力,且其華氏囊損傷程度最為輕微。因此,實驗結果證明大腸桿菌表現之VP243確實可以形成類病毒顆粒,且該次單位疫苗確實可以有效的於雞隻產生免疫反應,並提供良好之保護力對抗強毒株IBDV之感染。
The avian infectious bursal disease virus (IBDV) causes infectious bursal disease (IBD) in young chickens and is an important pathogen in poultry industry worldwide. The IBD is commonly controlled by vaccination with attenuated and / or inactivated vaccines in Taiwan. Researches have shown that the VP243 polyprotein which was expressed in eukaryotic cell could form a subviral particle and could induce protective immune responses. The objective of this study is to evaluate the immune responses and protection efficacy of chickens vaccinated by subunit vaccines containing E. coli expressed VP243 protein against very virulent IBDV (vvIBDV) challenge. The pTri-VP243 plasmids were constructed by cloning vp243 gene into pTriEx-3 vector and transformed into E. coli for protein expression. The soluble and insoluble VP243 proteins in the E. coli lysate were collected by centrifuging after IPTG induction. Both soluble and insoluble proteins were confirmed and analyzed by Western blotting, and the Virus-like particle (VLP) was confirmed by transmission electron microscope (TEM). Four animal trials were conducted and specific pathogen free (SPF) chickens were used in this objective. Chickens were intramuscularly vaccinated twice at one and three weeks of age with various types of antigens such as soluble VP243, insoluble VP243, soluble VP243 purified by 30 % sucrose or soluble VP243 mixed with different adjuvants. The chickens of vaccinated and challenge control groups were challenged with 4×102 EID50 / 200 μl of vvIBDV orally at one week after the last vaccination and euthanatized at 5 days after challenge. Blood was collected from each chicken weekly and the anti-IBDV antibody titers were measured by ELISA. At the termination day, a bursal gross lesion score was given for each chicken according to the severity of bursa damage. Prior to challenge, the groups vaccinated with various vaccines containing VP243 proteins have significantly higher anti-IBDV ELISA titers than that in the control groups (p < 0.05). The chickens vaccinated with VP243 soluble proteins which mixed with Freund''s Adjuvant showed well protection against vvIBDV with the similar bursal gross lesion scores as that in negative control groups (p > 0.05). The results demonstrated that the VP243 protein was successfully expressed by E. coli and provided protection of chickens against vvIBDV.
URI: http://hdl.handle.net/11455/66470
其他識別: U0005-2308201209055800
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-2308201209055800
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