Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/66475
DC FieldValueLanguage
dc.contributor徐維莉zh_TW
dc.contributorWei-Li Hsuen_US
dc.contributor.author歐俊麟zh_TW
dc.contributor.authorOu, Jun-Linen_US
dc.contributor.other微生物暨公共衛生學研究所zh_TW
dc.date2013en_US
dc.date.accessioned2014-06-09T09:31:59Z-
dc.date.available2014-06-09T09:31:59Z-
dc.identifierU0005-2106201317052700en_US
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dc.identifier.urihttp://hdl.handle.net/11455/66475-
dc.description.abstractCurcumin (薑黃素)常用來作為食品染色劑和香料。越來越多的證據表示,薑黃素具有抗癌、抗發炎、抗菌的特性,在醫學研究上特別受到關注。近期實驗室研究證明薑黃素可抑制感染A型流感病毒(IAV),其機制之一是透過干擾病毒的Hamagglutintin (HA)活性。本研究試圖透過比較分析薑黃素與其功能結構類似物,包含Tetrahydrocurcumin (THC)、Monoacetylcurcumin (MAC)和 Petasiphenol (Pet) 對於IAV複製之作用,以探究薑黃素的抗流感病毒之關鍵結構。在病毒感染不同時期加入藥物處理,結果顯示所有的類似物都能抑制IAV,其中MAC與薑黃素抑制IAV子代病毒產生能力相當接近,THC和Pet抑制能力比薑黃素差很多。此外,比較處理藥物後對於IAV感染力之影響,得知THC和Pet在抑制流感病毒斑形成能力的影響較弱; curcumin與MAC的抑制50 %病毒斑形成之濃度( EC50) 分別為0.17 μM及0.2 μM,Pet則為14.65 μM。但是除了薑黃素,所有測試的類似物均不具有抑制流感病毒HA蛋白質的活性。在結構上薑黃素與MAC在中央碳鍊具有兩個雙鍵(構成enone官能基),而Pet帶有一個,THC則是不具有enone官能基。實驗證明中央碳鏈上的兩個雙鍵可能是curcumin抗流感的關鍵結構。Enone group為Michael acceptor electrophile (MAE),會與胺基酸之S-H group交互作用,進而與蛋白質以共價鍵方式結合。 因此推測curcumin極有可能藉由Enone group與病毒蛋白結合,干擾病毒表面蛋白的功能和降低病毒的感染力。综上所述, 測試之薑黃素類似物中,薑黃素的穩定代謝物THC仍具有抑制IAV的能力,而MAC抗IAV能力不亞於薑黃素,具有開發為新型抗流感藥物之潛力。比較分析薑黃素結構類似物對於IAV之作用,發現薑黃素中央碳鍊的兩個雙鍵為抑制IAV之關鍵結構。zh_TW
dc.description.abstractCurcumin is a commonly used colouring agent and spice in food. Accumulated evidence indicates that curcumin is associated with a great variety of pharmacological activities, including an antimicrobial effect. Previously, we reported curcumin inhibits the infection of type A influenza virus (IAV) and proved one of the mechanisms is through interfering the viral HA activity. To investigate the structure contributing to its anti-IAV activity, structural and functional analogues of Curcumin (Cur), such as Tetrahydrocurcumin (THC), Monoacetylcurcumin (MAC) and Petasiphenol (Pet) were comparatively analyzed in the current study. The result of time-of-drug addition tests revealed that all the analogues analyzed were able to inhibit IAV production in cell cultures; MAC has a similar strength to curcumin, whereas Pet and THC inhibit IAV to a much less extent than curcumin. Comparative analysis of curcumin analogues described herein demonstrated that Thc and Pet have much less effect on suppression of plaque formation ability; the EC50 of pet are 14.65 μM, whereas curcumin and MAC is 0.17 and 0.2 μM, respectively. Surprisingly, none of the analogues harbors HA inhibition effect. Considering that as with curcumin, the structure of MAC contains two double bonds in the central seven-carbon chain, whereas Pet and THC has one or none, respectively, it indicates the presence of double bond is crucial for the anti-IAV activity. The unsaturated carbonyl group acts as the acceptor of Michael addition reaction that involves in the intermolecular conjugation by formation of covalent Michael adducts with certain proteins. It is very possible that via Michael addition reaction, curcumin conjugated with viral protein that in turns alters or interferes with the function of viral surface proteins and inactivate virus infectivity. In conclusion, THC, one of the stable curcumin metabolites exhibits anti-IAV activity and MAC appears to be an effective agent for inhibition of IAV infection. Moreover, comparative analysis of curcuminoids indicated that the two double bonds in the central seven-carbon chain contribute to the curcumin -mediated anti-IAV activity.en_US
dc.description.tableofcontents目次 第一章 文獻探討 3 第一節 流行性感冒病毒簡介 3 第二節 流行性感冒病毒特性 4 第三節 流感病毒結構 4 第四節 流感病毒生活史 (圖二) 8 第五節 流感病毒預防接種與抗流感藥物的治療 9 第六節 Curcumin (薑黃素)與其結構類似物 11 ㄧ、Curcumin簡介 12 二、Curcumin生物特性 12 三、Curcumin抗癌能力 13 四、curcumin抗病毒能力研究 14 五、Curcumin與其結構類似物 15 第二章 材料與方法 19 第一節 實驗材料與基本步驟 19 ㄧ、細胞 19 二、病毒株: 19 第二節 重要實驗設計與步驟 20 一、病毒斑分析(plaque assay) 20 二、病毒斑減低分析(plaque reduction assay) 21 三、血球凝集實驗 (Hemagglutination test ; HA test) 21 四、血球凝集抑制實驗 (Hemagglutination inhibition test ; HI test) 22 五、病毒TCID50測試 22 六、ARV病毒抑制測試 22 七、以time-of-drug addition方式測試藥物對PR8病毒感染影響 22 八、免疫螢光分析法 (Immunofluorescence assay ; IFA) 23 九、西方墨點法 (Western Blot analysis) 23 十、Glutathion (GSH)競爭curcumin與病毒蛋白質結合測試 24 第三章 結果 25 第一節 比較curcumin對於流感病毒 (strain PR8;以下簡稱PR8)與Avian reovirus的效力 25 第二節 Curcumin結構類似物的抗流感病毒能力 25 第三節 探討curcumin結構類似物對病毒感染不同時期影響 26 第四節 探討curcumin結構類似物對病毒蛋白質生成之影響 27 第五節 探討curcumin類似物是否可以抑制流感病毒HA 的活性 28 第六節 Curcumin (及類似物)是否藉由Michael addition conjugation影響到病毒感染 28 第四章 討論 30 參考文獻 34 附錄 57zh_TW
dc.language.isozh_TWen_US
dc.publisher微生物暨公共衛生學研究所zh_TW
dc.relation.urihttp://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-2106201317052700en_US
dc.subject流感病毒zh_TW
dc.subjectInfluenza virusen_US
dc.subject薑黃素zh_TW
dc.subject類似物zh_TW
dc.subject病毒班分析zh_TW
dc.subject病毒班減低分析zh_TW
dc.subject紅血球凝集素抑制zh_TW
dc.subjectα,β-不飽和醛酮zh_TW
dc.subjectcurcuminen_US
dc.subjectanaloguesen_US
dc.subjectplaque assayen_US
dc.subjectplaque reduction assayen_US
dc.subjectInhibition of hemagglutinationen_US
dc.subjectenoneen_US
dc.title比較分析薑黃素及其類似物的抗流感病毒活性zh_TW
dc.titleComparative analysis of curcumin analogues on anti-influenza virus activityen_US
dc.typeThesis and Dissertationzh_TW
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