Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/66656
標題: Engulfed pathogen-induced apoptosis in haemocytes of giant freshwater prawn, Macrobrachium rosenbergii
作者: Hsu, J.P.
宣詩玲
Huang, C.
Liao, C.M.
Hsuan, S.L.
Hung, H.H.
Chien, M.S.
黃千衿
簡茂盛
關鍵字: apoptosis
haemocytes
in vitro
Macrobrachium rosenbergii
pathogens
phagocytosis
penaeus-monodon
cell-death
necrosis
crustaceans
infection
mortality
immunity
system
virus
期刊/報告no:: Journal of Fish Diseases, Volume 28, Issue 12, Page(s) 729-735.
摘要: Haemocytes of the giant freshwater prawn, Macrobrachium rosenbergii, were investigated for the induction of apoptosis after phagocytosis of pathogenic yeasts, bacteria and non-pathogenic latex beads in vitro. Isolated haemocytes of M. rosenbergii were cultured at a ratio of 1:50 haemocytes to pathogen with the yeast Debaryomyces hansenii, the bacteria Aeromonas hydrophila or Enterococcus faecium, or with latex beads at 25 degrees C for 2 h, followed by washing to remove free particles. At least 200 haemocytes were counted to determine the phagocytosis rate, and the results showed that haemocytes engulfed latex beads at a higher rate than the aquatic pathogens. By transmission electron microscopy, the yeast- or bacterium-engulfing haemocytes displayed morphological changes characteristic of apoptosis, including formation of cytoplasmic vacuoles, chromatin condensation and fragmentation of nuclei. This pathogen-induced apoptosis was further confirmed by DNA laddering and TUNEL (terminal deoxynucleotidyl transferase-mediated deoxy-UTP nick-end-labelling) assays. Neither haemocytes treated with latex beads nor uninfected haemocytes (control group) showed signs of apoptosis after 48 h in culture.
URI: http://hdl.handle.net/11455/66656
ISSN: 0140-7775
文章連結: http://dx.doi.org/10.1111/j.1365-2761.2005.00681.x
Appears in Collections:微生物暨公共衛生學研究所

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