Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/66656
DC FieldValueLanguage
dc.contributor.authorHsu, J.P.en_US
dc.contributor.author宣詩玲zh_TW
dc.contributor.authorHuang, C.en_US
dc.contributor.authorLiao, C.M.en_US
dc.contributor.authorHsuan, S.L.en_US
dc.contributor.authorHung, H.H.en_US
dc.contributor.authorChien, M.S.en_US
dc.contributor.author黃千衿zh_TW
dc.contributor.author簡茂盛zh_TW
dc.date2005zh_TW
dc.date.accessioned2014-06-11T05:46:55Z-
dc.date.available2014-06-11T05:46:55Z-
dc.identifier.issn0140-7775zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/66656-
dc.description.abstractHaemocytes of the giant freshwater prawn, Macrobrachium rosenbergii, were investigated for the induction of apoptosis after phagocytosis of pathogenic yeasts, bacteria and non-pathogenic latex beads in vitro. Isolated haemocytes of M. rosenbergii were cultured at a ratio of 1:50 haemocytes to pathogen with the yeast Debaryomyces hansenii, the bacteria Aeromonas hydrophila or Enterococcus faecium, or with latex beads at 25 degrees C for 2 h, followed by washing to remove free particles. At least 200 haemocytes were counted to determine the phagocytosis rate, and the results showed that haemocytes engulfed latex beads at a higher rate than the aquatic pathogens. By transmission electron microscopy, the yeast- or bacterium-engulfing haemocytes displayed morphological changes characteristic of apoptosis, including formation of cytoplasmic vacuoles, chromatin condensation and fragmentation of nuclei. This pathogen-induced apoptosis was further confirmed by DNA laddering and TUNEL (terminal deoxynucleotidyl transferase-mediated deoxy-UTP nick-end-labelling) assays. Neither haemocytes treated with latex beads nor uninfected haemocytes (control group) showed signs of apoptosis after 48 h in culture.en_US
dc.language.isoen_USzh_TW
dc.relationJournal of Fish Diseasesen_US
dc.relation.ispartofseriesJournal of Fish Diseases, Volume 28, Issue 12, Page(s) 729-735.en_US
dc.relation.urihttp://dx.doi.org/10.1111/j.1365-2761.2005.00681.xen_US
dc.subjectapoptosisen_US
dc.subjecthaemocytesen_US
dc.subjectin vitroen_US
dc.subjectMacrobrachium rosenbergiien_US
dc.subjectpathogensen_US
dc.subjectphagocytosisen_US
dc.subjectpenaeus-monodonen_US
dc.subjectcell-deathen_US
dc.subjectnecrosisen_US
dc.subjectcrustaceansen_US
dc.subjectinfectionen_US
dc.subjectmortalityen_US
dc.subjectimmunityen_US
dc.subjectsystemen_US
dc.subjectvirusen_US
dc.titleEngulfed pathogen-induced apoptosis in haemocytes of giant freshwater prawn, Macrobrachium rosenbergiien_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1111/j.1365-2761.2005.00681.xzh_TW
Appears in Collections:微生物暨公共衛生學研究所
文件中的檔案:

取得全文請前往華藝線上圖書館



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.