Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/66675
標題: Differential diagnosis of orf viruses by a single-step PCR
作者: Chan, K.W.
徐維莉
Hsu, W.L.
Wang, C.Y.
Yang, C.H.
Lin, F.Y.
Chulakasian, S.
Wong, M.L.
王孟亮
王之仰
關鍵字: Orf virus
PCR
Diagnosis
polymerase-chain-reaction
vaccinia virus
interferon resistance
laboratory diagnosis
gene
goats
parapoxviruses
identification
outbreak
homolog
期刊/報告no:: Journal of Virological Methods, Volume 160, Issue 1-2, Page(s) 85-89.
摘要: The complete nucleotide sequence of the A32L gene (named after vaccinia virus, corresponding with open reading frame 108 of the orf virus and encoding an ATPase) of the orf virus was studied using samples of orf virus from infected goats, which were collected from six outbreaks in central Taiwan. DNA sequence analysis of the A32L genes of these and isolates from other countries showed sequence heterogeneity (base pair variation and deletion) in the 3'-terminal regions. This finding led to the development of a polymerase chain reaction (PCR) method for the rapid differential diagnosis of orf virus infections, and the results demonstrated that this was an easy and reliable method for genotyping of orf viruses. (C) 2009 Elsevier B.V. All rights reserved.
URI: http://hdl.handle.net/11455/66675
ISSN: 0166-0934
文章連結: http://dx.doi.org/10.1016/j.jviromet.2009.04.025
Appears in Collections:微生物暨公共衛生學研究所

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