Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/67690
標題: Acute anal stretch inhibits NMDA-dependent pelvic-urethra reflex potentiation via spinal GABAergic inhibition in anesthetized rats
作者: Chen, S.L.
Huang, Y.H.
Kao, Y.L.
Chen, G.D.
Cheng, C.L.
Peng, H.Y.
Liao, J.M.
Huang, P.C.
Tsai, S.J.
Lin, T.B.
關鍵字: spinal cord
voiding dysfunction
glutamate
bicuculline
hydroxysaclofen
lower urinary-tract
gaba-b receptors
intrathecal baclofen
descending
facilitation
presynaptic inhibition
clinical-significance
micturition
reflex
sphincter stretch
pontine tegmentum
pudendal nerve
期刊/報告no:: American Journal of Physiology-Renal Physiology, Volume 295, Issue 4, Page(s) F923-F931.
摘要: The impact of acute anal stretch on the pelvic-urethra reflex potentiation was examined in urethane-anesthetized rats by recording the external urethra sphincter electromyogram activity evoked by the pelvic afferent stimulation. Test stimulation (1 stimulation/30 s) evoked a baseline reflex activity with a single action potential that was abolished by gallamine (5 mg/kg iv). On the other hand, the repetitive stimulation (1 stimulation/1 s) induced spinal reflex potentiation (SRP) that was attenuated by intrathecal 6-cyano-7-nitroquinoxaline- 2,4-dione (a glutamatergic alpha-amino-3-hydroxy-5-methyl- 4-isoxazoleproprionat receptor antagonist, 100 mu M, 10 mu l) and D-2-amino-5-phosphonovalerate [a glutamatergic N-methyl-D-aspartate (NMDA) antagonist, 100 mu M, 10 mu l]. Acute anal stretch using a mosquito clamp with a distance of 4 mm exhibited no effect, whereas distances of 8 mm attenuated and 12 mm abolished the repetitive stimulation-induced SRP. Intrathecal NMDA (100 mu M, 10 mu l) reversed the abolition on SRP caused by anal stretch. On the other hand, pretreated bicuculline [gamma-aminobutyric acid (GABA) A receptor antagonist, 100 mu M, 10 mu l] but not hydroxysaclofen (GABA(B) receptor antagonist) counteracted the abolition on the repetitive stimulation-induced SRP caused by the anal stretch. All of the results suggested that anal stretch may be used as an adjunct to assist voiding dysfunction in patients with overactive urethra sphincter and that GABAergic neurotransmission is important in the neural mechanisms underlying external urethra sphincter activity inhibited by anal stretch.
URI: http://hdl.handle.net/11455/67690
ISSN: 1931-857X
文章連結: http://dx.doi.org/10.1152/ajprenal.90254.2008
Appears in Collections:期刊論文

文件中的檔案:

取得全文請前往華藝線上圖書館



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.