Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/67691
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dc.contributor.authorChang, C.H.en_US
dc.contributor.authorPeng, H.Y.en_US
dc.contributor.authorWu, H.C.en_US
dc.contributor.authorLai, C.Y.en_US
dc.contributor.authorHsieh, M.C.en_US
dc.contributor.authorLin, T.B.en_US
dc.date2011zh_TW
dc.date.accessioned2014-06-11T05:55:31Z-
dc.date.available2014-06-11T05:55:31Z-
dc.identifier.issn1931-857Xzh_TW
dc.identifier.urihttp://hdl.handle.net/11455/67691-
dc.description.abstractChang CH, Peng HY, Wu HC, Lai CY, Hsieh MC, Lin TB. Cyclophosphamide induces NR2B phosphorylation-dependent facilitation on spinal reflex potentiation. Am J Physiol Renal Physiol 300: F692-F699, 2011. First published November 24, 2010; doi:10.1152/ajprenal.00531.2010.-It is well-established that cyclophosphamide (CYP) can sensitize the pelvic afferent nerve arising from the urinary bladder and therefore induce suprapubic pain. To test the possibility that CYP might mediate the development of visceral hypereflexia/hyperalgesia by facilitating spinal activity-dependent neural plasticity, we compared the pelvic-urethra reflex activity and spinal N-methyl-D-aspartate receptor NR2B subunit (NR2B) phosphorylation in rats treated with vehicle solution and CYP. Compared with vehicle solution, when accompanied by upregulation of phosphorylated NR2B expression in the lumbosacral (L6-S2) dorsal horn, CYP increased the evoked spikes in spinal reflex potentiation induced by repetitive stimulation (1 stimulation/1 s). Moreover, intraperitoneal pretreatments with N(G)-nitro-L-arginine methyl ester and roscovitine, nitric oxide synthase and cyclin-dependent protein kinase 5 (Cdk5) antagonists, respectively, overwrote CYP-enhanced reflex potentiation and NR2B phosphorylation. When compared with the untreated group, the treatment with small-interfering RNA of NR2B, which decreased the expression of NR2B expression, abolished CYP-dependent reflex facilitation and spinal NR2B phosphorylation. These results suggested that CYP might facilitate spinal reflex potentiation mediated by N-methyl-D-aspartate receptors and participate in the development of visceral hypereflexia/hyperalgesia through nitric oxide-and Cdk5-dependent NR2B phosphorylation at the lumbosacral dorsal horn.en_US
dc.language.isoen_USzh_TW
dc.relationAmerican Journal of Physiology-Renal Physiologyen_US
dc.relation.ispartofseriesAmerican Journal of Physiology-Renal Physiology, Volume 300, Issue 3, Page(s) F692-F699.en_US
dc.relation.urihttp://dx.doi.org/10.1152/ajprenal.00531.2010en_US
dc.titleCyclophosphamide induces NR2B phosphorylation-dependent facilitation on spinal reflex potentiationen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1152/ajprenal.00531.2010zh_TW
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